Validated All-in-One™ qPCR Primer for KDM4A(NM_014663.2) Search again

Buy	Catalog#	Description	Note	Price (US$)
	HQP023073	All-in-One™ qPCR Primer for NM_014663.2 (200 reactions)	KDM4A	inquire
	HQP006940	All-in-One™ qPCR Primer for NM_002046.6 (200 reactions)	Reference Gene GAPDH	inquire
	HQP006940	All-in-One™ qPCR Primer for NM_002046.7 (200 reactions)	Reference Gene GAPDH	inquire
	HQP015171	All-in-One™ qPCR Primer for NM_004048.3 (200 reactions)	Reference Gene B2M	inquire
	HQP015171	All-in-One™ qPCR Primer for NM_004048.4 (200 reactions)	Reference Gene B2M	inquire
	HQP016381	All-in-One™ qPCR Primer for NM_001101.5 (200 reactions)	Reference Gene ACTB	inquire

Validated result:

A	B
Figure: All-in-One™ qPCR Primer Validated result to KDM4A. Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve.

Summary

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq].

Gene References into function