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Validated All-in-One™ qPCR Primer for ABCG1(NM_016818.2) Search again
Product ID:
HQP022988
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ABC8, WHITE1
Gene Description:
ATP binding cassette subfamily G member 1
Target Gene Accession:
NM_016818.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq].
Gene References into function
- ABCG1 is not required for apolipoprotein A-1-mediated endothelial cholesterol efflux from vascular endothelial cells.
- Analysis of the effect of the Su(Hw) insulator on the expression of the miniwhite gene in Drosophila melanogaster.
- ABCG1 redistributes cholesterol to cell-surface domains where it becomes accessible for removal by HDL, demonstrating its direct role of ABCG1 in cellular cholesterol transport
- characterization of two LXR response elements in first & second introns of the ABCG1 gene & demonstrate their function in cultured macrophage & liver cells; studies clarify mechanism of upregulation of the ABCG1 gene by oxysterols in macrophages & liver
- Abcg1 is a key gene involved in both cholesterol efflux to HDL and in tissue lipid homeostasis
- synergistic relationship between ABCA1 and ABCG1 in peripheral tissues, where ABCA1 lipidates any lipid-poor/free apoA-I to generate nascent or pre-beta-HDL
- ABCG1 was localized to the plasma membrane in HEK293 cells and mediate the efflux of cholesterol and choline phospholipids.
- provides the first evidence that unsaturated fatty acids suppress ABCG1 gene expression by a mechanism which involves the binding of LXR/RXR to the promoters
- Macrophage ABCG1 deficiency does lead to heavy lipid accumulation in macrophages of the lung, and also a moderately significant effect on atherosclerotic lesion development was observed.
- The splice variant ABCG1(666) may be the most prominent form of functional ABCG1 expressed in the human.
- ABCG1 and ABCG4 act in concert with ABCA1 to maximize the removal of excess cholesterol from cells and to generate cholesterol-rich lipoprotein particles
- NPC1 protein function is non-essential for the trafficking and removal of cellular cholesterol by ApoAI if the down-stream defects in ABCA1 and ABCG1 regulation in NPC disease cells are corrected using an LXR agonist
- Two genes of the cholesterol efflux system (ABCA1 and ABCG1) were down-regulated in HCAECs exposed to uraemic plasma.
- ABCG1 plays a significant role in the regulation of neuronal cholesterol efflux to apolipoprotein E discs.
- These results indicate that positional and geometrical isomers of CLAs have specific effects on gene expression of human macrophages and that t9,t11-CLA activates ABCG1 by a SREBP-1c-dependent mechanism.
- In intermediate phenotypes of suicidal behavior,ABCG1 might act on suicidal behavior through these traits.
- Results show that ABCG1 mRNA expression increased in response to dexamethasone in primary rat hepatocytes however, the effect was absent or inhibitory in human HepG2 cells and THP-1 macrophages due to low glucocorticoid receptor levels.
- altered cholesterol metabolism and amyloid precursor protein trafficking mediated by ABCG1 may contribute to the accelerated onset of Alzheimer's disease neuropathology in Down syndrome
- ABCG1 protects against 7beta-HC-induced cell death, an important role in prevention of neurodegenerative and cardiovascular disease.
- These results suggest that the ABCG1-mediated efflux of cholesterol and sphingomyelin (SM) is dependent on the cellular SM level and distribution of cholesterol in the plasma membrane.
- ABCG1 deficiency in pulmonary alveolar proteinosis alveolar macrophages is attributable to the absence of a GM-CSF-mediated PPARgamma pathway.
- No significant gender effect in the capacity of serum from nondyslipidemic subjects to mediate free cholesterol efflux via the ABCG1 transporter pathway was detected
- ABCG1 expression and cholesterol efflux are reduced in patients with type 2 diabetes mellitus
- Overexpression of human ABCG1 does not affect atherosclerosis in fat-fed ApoE-deficient mice.
- Results suggest a causative relationship between ABCG1 function and apoptotic cell death in macrophages and in other cell types.
- The reduction in ABCG1 is associated with impairment in cholesterol efflux and may contribute to accelerated foam cell formation in diabetic patients.