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Validated All-in-One™ qPCR Primer for NR1D1(NM_021724.4) Search again
Product ID:
HQP022925
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EAR1, REVERBA, REVERBalpha, THRA1, THRAL, ear-1, hRev
Gene Description:
nuclear receptor subfamily 1 group D member 1
Target Gene Accession:
NM_021724.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- novel physiological role for members of the Rev-erb family of nuclear receptors in the regulation of genes involved in triglyceride metabolism and the pathogenesis of atherosclerosis
- ROR(alpha) and Reverb(alpha) are expressed with a similar tissue distribution and are both induced during the differentiation of rat L6 myoblastic cells
- hRev-erbalpha plays a key role in hRORalpha1 action
- Data show that Rev-erb alpha and beta contain a functional nuclear location signal in the DNA-binding domain, and suggest that they control their intracellular localization via a mechanism different from that of other nuclear receptors.
- Rev-Erbalpha as a target gene of PPARgamma in adipose tissue and demonstrate a role for this nuclear receptor as a promoter of adipocyte differentiation.
- Overexpression of EAR1 upregulated expression of IL6 and COX2, and increased transactivation by NFKB and nuclear translocation of p65 in A7r5 VSMCs. The expression of EAR1 was upregulated by RORalpha1 but that upregulation was attenuated by EAR1 itself
- the N-CoR/HDAC3 complex has a role in regulating the expression of genes involved in circadian rhythm by functioning as corepressor for Rev-erbalpha
- findings show that GSK3beta phosphorylates and stabilizes Rev-erbalpha, a negative component of the circadian clock; control of Rev-erbalpha protein stability is a critical component of the peripheral clock and a biological target of lithium therapy
- Rev-ErbAalpha is highly expressed in osteoarthritis articular chondrocytes and that its expression is modulated by known cartilage catabolic and anabolic stimuli
- findings show that rev-erbalpha serves as a heme sensor that coordinates the cellular clock, glucose homeostasis, and energy metabolism
- heme regulation of REV-ERBs may link the control of metabolism and the mammalian clock
- Rev-erbalpha protein levels must rise and then fall for adipocyte differentiation to occur. Stable expression of Rev-erb protein prevents induction of PPARgamma2.
- These data identify Rev-erbalpha as a new LXR target gene, inhibiting LXR-induction of TLR-4 in a negative transcriptional feedback loop, but not cholesterol homeostasis gene expression.