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Validated All-in-One™ qPCR Primer for ENTPD1(NM_001776.5) Search again
Product ID:
HQP022883
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ATPDase, CD39, NTPDase-1, SPG64
Gene Description:
ectonucleoside triphosphate diphosphohydrolase 1
Target Gene Accession:
NM_001776.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- NTPDase/E-ATPDase activity was demonstrated on cryosections of human pancreas.Significantly diminished activity of NTPDase1 in the tissues surrounding the ducts was detected
- Thrombin-induced deactivation of CD39 in endothelial cells is reversed by HMG-CoA reductase inhibitors and preservation of ATP and ADP metabolism.
- Roles of Asp54 and Asp213 in Ca2+ utilization by soluble enzymes
- Depolarization causes the endothelial production of superoxide, which inhibits the activity of endothelial NTPDase-1 and enhances platelet aggregation.
- Correlation was observed between ATP hydrolysis and triglycerides in patients with chronic heart disease, suggesting a relationship between ATP diphosphohydrolase and thrombogenesis.
- hCD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism
- capacity of NTPDase1 to hydrolyze both nucleoside triphosphates and diphosphates.
- The NTPDase1/CD39 is the dominant ecto-nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type-2 purinergic (P2) G-protein coupled receptors (GPCRs).
- After exercise, all subjects showed a significant reduction of CD39 expression in platelet and an increase of CD39 expression in B lymphocytes.
- there is a functional link between the localization of CD39 in cholesterol-rich domains of the membrane and its role in thromboregulation
- leukocyte NTPDase1 provides means of dephosphorylating ATP which enables ATP-induced platelet aggregation via conversion to ADP, but also converts ADP to AMP and adenosine.
- Changes in the expression of NTPDase1 and caveolins seem to be independent of human cardiovascular disease
- CD39 associations with RanBPM have the potential to regulate NTPDase catalytic activity. This intermolecular interaction may have important implications for the regulation of extracellular nucleotide-mediated signalling.
- Distinct roles for CD39 and P2-purinergic signaling in both tissue remodeling and fibrogenesis with respect to human pancreatic diseases.
- Composition of the active site of wild-type CD39 appears optimized for ADPase function in the context of the transmembrane domains.
- Patients with the remitting/relapsing form of multiple sclerosis have strikingly reduced numbers of CD39(+) Treg cells in the blood
- Data show that host-derived CD39 is acquired by both laboratory-adapted and clinical variants of HIV-1 produced in cellular reservoirs of the virus.
- the effect of overexpressed CD39/NTPDase-1 in injured aorta
- Prolonged exposure to endogenous ATP related to decreased NTPDase1/CD39 activity leads to P2-purinoceptor desensitization in impotent men
- E-NTPDase1 plays an important role in regulating neutrophil chemotaxis by facilitating the hydrolysis of extracellular ATP
- a novel Sp1-dependent regulatory pathway for CD39 indicate the likelihood that CD39 is central to protective responses to hypoxia/ischemia
- stable oxidants present in diluted aqueous cigarette smoke extract (aCSE) are responsible for platelet NTPDase inhibition induced by aCSE.