|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for CD38(NM_001775.3) Search again
Product ID:
HQP022870
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ADPRC 1, ADPRC1, cADPR1
Gene Description:
CD38 molecule
Target Gene Accession:
NM_001775.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling. [provided by RefSeq].
Gene References into function
- Low CD38 expression parallels a lower propensity to apoptosis. In vivo CD38 crosslinking by its ligand may play a pivotal role in B-CLL apoptosis, and chlorambucil affects CD38 levels.
- CD38 plays a role in the progression of B-chronic lymphocytic leukemia and can be used as a prognostic marker
- expression level does not change in B-cell chronic lymphocytic leukemia
- CD38 expression and the consequential accumulation of cADPR play a causal role in mediating cellular differentiation
- CD38 receptor-mediated signals operate directly suggesting a Fcgamma receptorial surface molecule independent activation pathway. The key element for the receptor mediated signaling is represented by surface density of CD38 on resting monocytes.
- CD38 is not merely a marker in B-CLL, but plays a receptor role with pathogenetic potential ruling the proliferation of the malignant clone.
- results suggest that CD38 is superior to myeloid-associated markers in predicting the prognosis of patients with B-CLL
- CD38 enhances thymocyte death by interacting with CD31 expressed by accessory cells and may play a role in passive immunity.
- CD38 operates in two functionally distinct microdomains of the plasma membrane
- findings indicate a novel negative feedback mechanism between RyR1 channel activity and CD38 abundance acts in cADPr signal transduction in lymphoma b-cells
- CD38 overexpression is associated with B-cell chronic lymphocytic leukemia
- Expression of CD38 on CD8+ T cells is associated with poor prognostic outcome in hiv infected individuals with detectable plasma viremia
- cADPR, in concert with IP(3), operates in airway gland acinar cells to mobilize Ca(2+), resulting in Cl(-) secretion.
- concentrative influx of extracellular cADPR across CNT3 and cs-csg NT could substitute in the absence of CD38 in eliciting cADPR-dependent [Ca(2+)](i) increases in granulocyte-differentiated HL-60 cells, as well as in other CD38(-) cells
- CD38 is stimulated by sequential activation of IL8 receptor, IP(3)-mediated Ca(2+) rise, and cGMP/protein kinase G and plays an essential role in IL8-induced migration of LAK cells
- CD38, a negative prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL), plays a role in neoplastic B-cell growth and survival.
- In the current study, ZAP-70 and CD38 expressions were examined in 252 patients with B-CLL. Surface CD38 expression on more than 30% (CD38(+)) of B-CLL cells were associated with an unfavorable clinical course.
- The level of CD38 expression on different T-cell subsets is differentially upregulated in drug-naive HIV-infected patients.
- leukocyte antigen-DR, CD38, and CD9 share a common pathway of tyrosine kinase activation in human monocytes
- CD38 expression is augmented in ex vivo CD3+, CD4+, CD8+, and CD25+ systemic lupus erythematosus T cells, which correlates with its increased insolubility in Brij 98 detergent, and its translocation into lipid rafts.
- The crystal structure of the soluble extracellular domain of human CD38 to 1.9 A resolution was determined.
- localization of CD38 in lipid rafts and its multiple interactions with signaling receptors rule innate and adaptive immune responses by tuning DC migration, survival, and Th1-polarization ability
- Retinoic acid-induced CD38 expression in HL-60 myeloblastic leukemia cells regulates cell differentiation or viability depending on expression levels
- anti-CD38 autoantibodies are more prevalent in long-standing than in new-onset type 1 diabetes, and more prevalent in type 2 than in type 1 diabetes. (review)
- Results showed a simple method of quantitation of CD38 expression to be sufficient to identify patients with an unfavourable prognosis in B cell chronic lymphocytic leukaemia.
- TNF-alpha-induced CD38 expression involves NF-kappaB expression and its activation and dexamethasone inhibits CD38 expression through NF-kappaB-dependent and -independent mechanisms.
- TRPM2 is a potential molecular target for cADPR, which regulates Ca(2+) entry into pancreatic beta-cells at body temperature depending on the production of cADPR-related molecules, thereby regulating insulin secretion.
- B-prolymphocytic leukemia appears biologically heterogeneous regarding IgVH mutations, ZAP-70 and CD38 expression, showing a pattern distinct from that of other lymphoproliferative disorders
- Combined analysis of CD38 in B-chronic lymphocytic leukemia and T cells is superior in predicting outcome of male B-CLL patients.
- results show that, in lymphoma cells, propensity to apoptosis was significantly linked to CD38 expression and that, remarkably, such response was independent of the nature of the trigger used
- These results suggested that the N-linked glycosylation of CD38 plays a crucial role in the structure stability by preventing the formation inter-molecular cross-links.
- active sites of CD38 and ADP-ribosyl cyclase have roles in nicotinic acid adenine dinucleotide phosphate (NAADP) synthesis and hydrolysis activities
- IRTA4/FcRH2 expression as detected by flow cytometry was significantly lower in the poor prognosis subgroup as compared to ZAP-70-CD38- B-chronic lymphocytic leukemia cells
- We found a low incidence of CD38+ CLL patients, and CD38 expression predicted significantly a more advanced stage of the disease, shorter lymphocyte doubling time and shorter therapy- and progression-free time.
- somatic mutation status and CD38 retained prognostic significance on multivariate analysis whereas ZAP-70 did not
- These results indicate that cADPR is an intracellular messenger of Ca(2+) signalling, suggesting that CD38 can contribute to mAChR-cADPR signalling.
- Review. CD38 senses extracellular NAD release in infection/inflammation & produces cADPR. This regulates calcium signaling & chemotaxis in monocytes, neutrophils and dendritic cells.
- results reveal that CD38 has a key role in neuropeptide release, thereby critically regulating maternal and social behaviours, and may be an element in neurodevelopmental disorders
- biological rationale for the poor prognosis of CD38(+) for chronic lymphocytic anemia patients.
- regulation of CD38 expression through p38 and JNK MAPKs involves NF-kappaB and AP-1 activation, and ERK and and p38 MAPKs also regulate expression posttranscriptionally through message stability
- lipid microdomain disruption and silencing of CD19 directly impacts on CD38's ability to mediate Ca(2+) fluxes, while leaving its surface expression unchanged
- Borrelia garinii blocks the up-regulation of CCR7 and CD38, important molecules in DC migration to lymph nodes, thus affecting further immune responses in Lyme borreliosis infection.
- Prognostic significance of combined analysis of ZAP-70 and CD38 in chronic lymphocytic leukemia was performed.
- analysis of catalysis-associated conformational changes revealed by human CD38 complexed with a non-hydrolyzable substrate analog
- CD38 expression labels an activated subset within chronic lymphocytic leukemia clones enriched in proliferating B cells.
- CD38 at the interchromatin region whether linked to nuclear scaffold or stored in nuclear structures as micronuclei and Cajal bodies co-localizing with coilin, suggests its involvement in nuclear processes.
- Erythrocyte NAD glycohydrolase and ADP-ribosyl cyclase activities were significantly increased in cancer, in parallel to enhancement of CD38 expression and in correlation with CEA values and anemia
- Results suggest that Zap-70 expression may be a better indicator of the mutational status of IgVH and prognosis of chronic lymphocytic leukemia than CD38 expression.
- CD38 and ZAP-70 are expressed in lymph node leukemic cells in both good and poor prognosis patients
- staining for TCL1, CD38, and CD44 are useful ancillary tests to identify B-cell tumors
- These results indicate a critical role for CD38 in TNFalpha-induced enhancement of [Ca(2+)](i) in human airway smooth muscle cells.
- a new role for CD38 in modulating antigen-mediated T-cell responses during IS formation
- association with B-cell chronic lymphocytic leukemia progression in western and asian enthnic populations
- Regardless of CD38 expression level, CD38 and ZAP-70 expressions were significantly associated in B-CLL
- CD38 expression was increased by isonicotinic acid and all-trans-retinoic acid
- Proliferating tumor cells in lymph nodes express CD38, which is in turn associated with an increased number of CD31(+) vascular endothelial cells
- functional role of NF-kappaB, AP-1 and glucocorticoid response elements in the cd38 promoter in the transcriptional regulation of CD38
- CD38 is a dependable negative prognostic marker for chronic lymphocytic leukemia;In conjunction with chemokines and their receptors, CD38 influences cell migratory responses; (Review)
- highest G allele frequency was observed in the 16 patients of the cohort that developed Richter syndrome
- Data suggest that detection of CD2 or CD13 expression in chronic lymphocytic leukemia (CLL) suggests familial CLL, and that CD38 expression does not carry the negative prognosis observed in sporadic CLL.
- Glucocorticoid regulation of CD38 expression in human airway smooth muscle cells: role of dual specificity phosphatase 1
- There was a significant correlation between the percentage of leukemic cells positive for ZAP-70 and the percentage of CD38+CD19+ cells (R=0.629; p=0.000001).
- data do not support an important contribution of CD38 triggering by CD31 to the proliferative and antiapoptotic state of the leukemic clone
- CD38(+) is a stable parameter for the identification of chronic lymphocytic leukemia patients with a more aggressive disease
- CD38-cyclic adenosine diphosphate-ribose (cADPR)-mediated increase in astrocyte intracellular divalent calcium ions plays an important role in potentiating neurotoxicity in HIV-1-associated neurocognitive disorder.
- CD38 was a good predictor of OS in Chinese patients with CLL.
- positive ZAP-70 and CD38 status were associated with an unfavorable clinical course in chronic lymphocytic leukemia patients
- the presence of nuclear CD38 by immunofluorescence and confocal microscopy using a panel of hematopoietic cell lines that exhibit different levels of CD38 plasma membrane expression.
- human CD38, an NAD-utilizing enzyme, is capable of catalyzing the cleavage reactions through both covalent and noncovalent intermediates, depending on the substrate used
- Simultaneous analysis of ZAP-70, CD38 and IGHV mutations in CLL provides more discriminatory prediction of treatment-free interval than any factor alone
- that CD38 undergoes dramatic structural changes at the receptor region and at the enzymatic region in the presence of calcium.