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Validated All-in-One™ qPCR Primer for CD34(NM_001773.2) Search again
Product ID:
HQP022813
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
-
Gene Description:
CD34 molecule
Target Gene Accession:
NM_001773.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM].
Gene References into function
- CD34 positive blood cell counts predict leukapheresis cd34 graft content for stem cell transplantation.
- These findings demonstrate that cells with variable repopulation potential comprise the human CD34(+) population and that short- and long-term potential of human precursors can be evaluated in the mouse model
- CD34 is a surface receptor molecule that is modulated by receptor-mediated endocytosis.
- Differential regulation of the human and murine CD34 genes in hematopoietic stem cells
- Immature CD34+CD19- progenitor/stem cells in TEL/AML1-positive acute lymphoblastic leukemia are genetically and functionally normal.
- CD34 antigen has 3 epitopes differeing in sensitivity to enzymatic cleavage. These are expressed differently in different subtypes of acute myeloid leukemia and correlate with prognosis for disease-free survival.
- Expression in uterine leiomyoma
- The human CD34 locus from a human PAC library was characterized & expressed in stable cell lines & transgenic mice. A 160-kb fragment (110 kb of 5' & 26 kb of 3' flanking regions) directs hCD34 expression in the bone marrow of transgenic mice.
- CD34 expression is reversible on human hematopoietic stem/progenitor cells
- Regions between 10 to 18 kb upstream and/or 17 to 26 kb downstream of the human CD34 gene contain critical elements for human CD34 expression in vivo.
- Expression in acute promyelocytic leukemia is a unique clinical feature associated with leukocytosis and atypical morphology with hypogranular pattern and is associated with a poor clinical outcome
- CD34-positive stromal cells may play a supportive role in primary lung adenocarcinomas
- Solitary sclerotic fibroma is a fibrotic lesion with cells positive for CD34 and O13.
- Wild-type fms-related tyrosine kinase 3-overexpressing CD34+ cells exposed to high levels of its physiologic ligand did not produce early cobblestone areas
- Cord blood cells contining cd34 antigens are can be expanded from placenta-derived mesenchymal progenitor cells.
- CD34 antigen may play a role in hepatocarcinogenesis in patients with HBV-associated chronic liver diseases
- Measurement of CD34 expression by LIN negative cells in expansion culture underestimates the total content of hematopoietic cells.
- constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells may be involved in the regulation of trafficking the human hematopoietic progenitor/stem cells to and in the bone marrow microenvironment
- CD34 may have a role in apoptosis and drug resistance in acute myeloid leukemia and myelodysplastic syndromes
- The CD34 Antigen Expression was quantified using computerised image analysis.
- resistance to CD95-mediated apoptosis in CD34+ progenitor cells might be controlled by caspase-8L expression in CD34+ progenitor cells
- These results suggest that a co-culture of CD34+ cells with the MSCs might not simply deliver the proliferation signals but also stemness and survival signals, and overlap the action of epigenetic regulators.
- mobilized CD34+ cells do not reside in G0, but in early G1 phase, and did not cross the restriction point into late G1 phase
- patients with myelophthisis have increased circulating CD34+ cells
- potential favourable effect of circulating CD34(+) cells on left ventricular function.
- Findings suggest that CD34-positive mesenchymal cells are an integral component of elastofibroma, which represents a clonal fibrous proliferation.
- The expression of beta-catenin, p63 and CD34 in the course of androgenetic alopecia is reported.
- myeloma cells have plasticity expressed by their ability to reprogram, dedifferentiate, and acquire autonomous survival properties, as shown by the loss of CD34 expression in cocultured cells
- expression of CD34 is of potential diagnostic value in the distinction between benign and malignant tumors in some difficult cases.
- Results suggest that the interaction of the cytoplasmic tail of CD34 with the shallow proline-rich motif-binding groove of Crk-L is essential for the function of CD34 in stem cell development.
- CD34 immunostaining provides some insights into the histogenesis of invasive and noninvasive implants in serous borderline tumors of the ovary.
- new CD34 cell subpopulation, which is apparently a precursor classical endothelial progenitor cells more potent with respect too homing and vasscular repair.
- Expression of HIF-1alpha mRNA was closely correlated with the expression of survivin, PCNA, and CD34 proteins in pancreatic cancer.
- bone marrow stem cells contribute to the growing tumor vasculature in Ewing's sarcoma and that vascular endothelial growth factor-165 is critical for the migration of CD34+ cells from the bone marrow into the tumor
- The novel CD34+-derived DC represent attractive candidates for use in adoptive T-cell immunotherapy.
- Results demonstrate that the optimum temperature for maintaining the viability of CD34+ cells, during overnight storage and transport of freshly harvested hematopoietic Progenitor Cells, is 2-8 degrees C.
- Positivity of CD117 and CD34 is the most valuable factor in diagnosing gastrointestinal stromal tumor (GIST).
- should be valuable to study virus-induced pathology and to rapidly evaluate new approaches aiming to prevent or treat HIV infection
- The interactions of platelets and CD34+ progenitor cells in the generation of foam cells and endothelial cells are reported.
- trisomy 8 cells undergo incomplete apoptosis
- a lack of integrin engagement leads to the induction of cellular markers associated with myeloid differentiation
- Interaction between CD34+ cells may coordinate cell function and participate in the control of hematopoietic stem/progenitor cell division kinetics.
- In conclusion, we report herein a new two-step culture method that leads to high yields of mature DC without any need of CD34+ HSC enrichment.
- The differences between the gene expression profiles of CD34(+) and CD133(+) cells indicate the more primitive nature of CD133(+) cells suggesting that CD34(+) and CD133(+) cells may have different roles in hematopoietic regeneration.
- No siatistically significant value in assocation with survival or event-free survival in head and neck squamous cell carcinoma.
- CD34 immunoreactivity closely parallel both the different morphologic steps of melanocytic tumor progression and the presence of histological parameters related to the aggressive behaviour.
- Results indicate that CD43 could be a potential candidate for differentiating chronic myeloid leukemia and acute promyelocytic leukemia cells.
- most likely outcome of a single nuclear traversal of a single alpha particle in CD34+ cells is a single chromosome aberration per damaged cell
- cord blood-derived CD34+ cells have a role in multiorgan dysfunction during experimental heatstroke
- CD34 can be a good marker for the enrichment of progenitor populations of liver cells.
- Loss of CD34+ fibrocytes is not a consistent feature of invasive lobular carcinomas of the breast. A preserved CD34+ stromal cell population does not exclude malignancy.
- VEGF appeared more common as compared to CD 34 in early liver fibrosis.
- Length of apheresis adapted to the richness in the peripheral blood CD34+ cells allows for optimizing the organization of centers with an improvement in patient comfort and economical savings.
- CD34 was determined in a variety of cutaneous neoplasms.
- SARS-infected lung cells express the stem/progenitor cell markers CD34 and Oct-4.
- Circulating CD34+ cell number was found to significantly correlate with plasma brain natriuretic protein level, a marker of left ventricular dysfunction.
- VEGF, HIF-1alpha and CD34 expressions were more common in gastric tumors without serosal invasion
- higher levels found in the placentas of pre-eclamptic compared with normotensive women
- CD34(+) cells significantly increased in peripheral blood after exposure to moderate altitude in healthy individuals.
- analysis of CD34 antigen distribution in nonactivated cord blood cells
- CD13 was expressed in 73% of acute myeloid leukemia patients, CD15 was expressed in 43% of patients, CD33 was expressed in 64% of patients, and CD34 was expressed in 66% of patients.
- common transducer molecule of interleukin-6 family cytokines gp130 was expressed on stem (CD34+) cells in all the cases
- Increase in CD34-positive cells may be functionally correlated with the increase in ANP production in idiopathic dilated cardiomyopathies.
- Human umbilical cord-derived CD34(+) cells can be used as a prophylactic agent for experimental heatstroke.
- Due to the potentially better safety profiles, these chimeric antibodies might become alternatives to mouse anti-CD34 antibodies routinely used for clinical application.
- a maximal bout of exercise induces a significant shift in CD34+ cells toward CD34+/KDR+ cells
- Human cord blood CD34(+) cells cocultured with murine cardiomyocytes formed cells that exhibited excitation-contraction coupling features similar to those of cardiomyocytes.
- increased level of CD34(+) cells associated with ischemic stress is correlated with neovascularization at human ischemic brain
- Targeting human CD34+ hematopoietic stem cells with anti-CD45 x anti-myosin light-chain bispecific antibody preserves cardiac function in myocardial infarction.
- Describe reproducible flow cytometric endothelial progenitor cell determination in human peripheral blood as CD34+/CD144+/CD3- lymphocyte sub-population.
- STAT5 downregulation in CD34+ cells promotes megakaryocytic development, whereas activation of STAT5 drives erythropoiesis
- abnormal methylation of the CXCR4 promoter is a mechanism contributing to constitutive migration of CD34(+) cells in primary myelofibrosis
- Suggest that mobilized CD34+ cells may be a biomarker candidate for the efficacy of combined maximal tolerance dose and continuous infusional chemotherapy and G-CSF surge in gastric cancer.
- Coculture with human stromal cells induced expression of CD34 and CXCR4 on CB Lin(-)CD34(-) cells.
- Immunostaining for calretinin and CD34 is helpful in the diagnosis of endometrial polyp and hyperplasia.
- Increased CD34 expression is associated with pediatric T-cell acute lymphoblastic leukemia.
- levels of circulating CD34(+) cells have prognostic value for neural function, consistent with their potential role in maintaining cerebral circulation
- The normal dermis and benign melanocytic nevi showed numerous CD34(+) fibrocytes, whereas malignant melanomas were devoid of this cell type.
- CD34 was positively correlated with infiltration and lymph node status in gastric carcinoma.
- humanized mice showed low levels of human engraftment in the intestinal tract and seem to be resistant to rectal transmission of HIV
- Bortezomib up-regulated TRAIL receptor expression on quiescent CD34(+) chronic myeloid leukemia cells