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Validated All-in-One™ qPCR Primer for SLC22A8(NM_004254.3) Search again
Product ID:
HQP022635
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
OAT3
Gene Description:
solute carrier family 22 member 8
Target Gene Accession:
NM_004254.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. [provided by RefSeq].
Gene References into function
- elucidation of the molecular mechanism for renal tetracycline transport by human organic anion transporters (hOATs) using proximal tubular cells stably expressing hOATs
- polymorphisms in the OAT3 gene did not appear to be associated with changes in renal and tubular secretory clearance of pravastatin
- OAT3 plays an important role for anionic drug secretion in patients with renal diseases; expression levels of drug transporters such as OAT3 may be related to the alteration of renal drug secretion.
- data suggest that genetic variation in OAT3 may contribute to variation in the disposition of drugs
- The clustering of OAT genes in the genome raises the possibility that nucleotide polymorphisms in SLC22A6 could also effect SLC22A8 expression, and vice versa.
- These results indicate that the tissue-specific expression of hOAT3 might be regulated by the concerted effect of genetic (HNF1alpha and HNF1beta) and epigenetic (DNA methylation) factors.
- Both hOAT1 and hOAT3 are responsible for the basolateral uptake of edaravone sulfate in the kidney.
- urinary excretion of topotecan hydroxyl acid is accounted for by transport via OAT3, as well as glomerular filtration, in both rats and humans
- Inhibition of renal uptake (via OAT3) and efflux processes (via MRP2 and MRP4) explains the possible sites of drug-drug interaction for methotrexate with probenecid and some NSAIDs, including their glucuronides.
- hOAT3 contributes to the renal uptake of rosuvastatin
- The five regulatory single nucleotide polymorphism(SNP)s of OAT3 identified in nephrectomized patients are unlikely to influence OAT3 mRNA expression or promoter activity.