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Validated All-in-One™ qPCR Primer for KL(NM_004795.3) Search again
Product ID:
HQP022615
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HFTC3, KLA
Gene Description:
klotho
Target Gene Accession:
NM_004795.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq].
Gene References into function
- Association of human aging with a functional variant of klotho.
- Klotho gene polymorphism is associated with bone density and spondylosis of the lumbar spine in Japanese postmenopausal women.
- Klotho polymorphisms & bone density in white and the Japanese postmenopausal women found 11 polymorphisms;3 were common in both populations. Klotho may be involved in the pathophysiology of bone loss with aging in humans.
- Expression of klotho RNA was greatly reduced in kidneys of all chronic renal failure patients. Dietary P(i) restriction induced klotho expression, which enhances beneficial effect of P(i) restriction in patients with CRF and/or on haemodialysis. Review.
- Cross-sectional and prospective studies confirm a genetic model in which the KL-VS allele confers a heterozygous advantage in conjunction with a marked homozygous disadvantage for HDL-C levels, systolic blood pressure, stroke, and longevity.
- several single nucleotide polymorphisms in bone morphogenic protein 6, annexin A2, and klotho were associated with sickle cell osteonecrosis
- The Klotho gene has been identified as one of the genes that may regulate cirulating osteocalcin levels.
- Our study suggests that Klotho acts as a humoral factor to reduce H(2)O(2)-induced apoptosis and cellular senescence in vascular cells.
- No evidence that the single nucleotide polymorphism F352V that defines the KL-VS variant of KLOTHO is involved in the genetic susceptibility to type 2 diabetes in a large case-control and family-based study.
- the F352V Klotho polymorphism is associated with bone mineral density in postmenopausal women, suggesting that Klotho gene variants influence skeletal aging
- KLOTHO G395A polymorphism was associated with blood pressure and KLOTHO C1818T polymorphism was associated with glucose metabolism
- the KLOTHO gene is a candidate gene of atherosclerosis in humans
- Klotho normally regulates cellular senescence by repressing the p53/cyclin-dependent kinase inhibitor 1A pathway
- common mechanism of KLOTHO down-regulation, but executed at various times in life, may underlie both physiological and disease-related T cell aging
- allelic variants of Klotho constitute one of the genetic factors influencing bone mineral density in male adults.
- one variant in KLOTHO gene is associated with the susceptibility of hand osteoarthritis and appears to act through osteophyte formation rather than cartilage damage
- key factor regulating mineral and vitamin D metabolism
- Klotho/betaKlotho have evolved as a compensatory mechanism for the poor ability of heparin/heparan sulfate to promote binding of fibroblast growth factor 19, -21, and -23 to their cognate receptors
- failure to find any significant association between leukocyte telomere length and 10 single nucleotide polymorphisms in two ageing-related candidate genes, TGFB1 and KLOTHO
- believe to be the first evidence that loss-of-function mutations in human KL impair FGF23 bioactivity, underscoring the essential role of KL in FGF23-mediated phosphate and vitamin D homeostasis in humans
- a functional FGF19 receptor may consist of FGF receptor (FGFR) and heparan sulfate complexed with either alphaKlotho or betaKlotho
- elevated alpha-Klotho level mimics aspects of the normal response to hyperphosphatemia and implicate alpha-Klotho in the selective regulation of phosphate levels and in the regulation of parathyroid mass and function
- An association was observed between SNPs G395A and C2998T of the KLOTHO gene and knee osteoarthritis.
- a noncanonical PPAR-responsive element within the 5'-flanking region of the human klotho gene
- might play pivotal roles in minerametabolism as regulators that integrate calcium and phosphate homeostasis. (review)
- This review summarizes recent progress in understanding of Klotho function in the regulation of tissue-specific metabolic activity of the endocrine fibroblast growth factors--REVIEW
- These data provide new insights into the physiological roles of FGF-23 and Klotho.
- Type I membrane klotho expression is decreased and inversely correlated to serum calcium in primary hyperparathyroidism.
- review of expression and functions of klotho and the upregulation of klotho by PPARG
- klotho is a potential tumor suppressor and an inhibitor of the IGF-1 pathway and activator of the FGF pathway in human breast cancer