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Validated All-in-One™ qPCR Primer for MYOCD(NM_153604.3) Search again
Product ID:
HQP022608
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
MGBL, MYCD
Gene Description:
myocardin
Target Gene Accession:
NM_153604.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Myocardin is a smooth and cardiac muscle-specific transcriptional coactivator of serum response factor. When expressed ectopically in nonmuscle cells, myocardin can induce smooth muscle differentiation by its association with serum response factor (SRF; MIM 600589).[supplied by OMIM].
Gene References into function
- Myocardin is a critical serum response factor cofactor regulating smooth muscle cell differentiation; forced expression of myocardin activated expression of SM22 alpha, smooth muscle alpha-actin, and calponin-h1 genes in mouse ES cells
- HERP1 and myocardin expression was localized to smooth muscle cells in the neointima in human coronary atherosclerotic lesions.
- myocardin-MYOCD overexpression in small cerebral arteries appears to initiate independently of amyloid beta-peptide a pathogenic pathway mediating arterial hypercontractility
- Myocardin is frequently repressed during malignant transformation, contributing to a differentiation defect
- the SRC3-myocardin interaction is a site of convergence for nuclear hormone receptor-mediated and VSMC-specific gene regulation
- Data show that fibroblasts from human postmyocardial infarction scars acquire properties of cardiomyocytes after transduction with a recombinant myocardin gene.
- Forced expression of the myocardin (MyoC) gene in human ventricular scar fibroblasts leads to MyoC-dependent activation of genes that encode connexins, strongly enforcing intercellular electrical coupling.
- data suggest that functional natural myocardin promoter variation might be a genetic factor contributing to inter-individual differences in the development of cardiac hypertrophy
- Myocd is sufficient for the establishment of a SMC-like contractile phenotype.
- Foxo3a could negatively regulate myocardin expression levels through up-regulating catalase and the consequent reduction of ROS levels
- A rare human sequence variant reveals myocardin autoinhibition
- We suggest that SRF and MYOCD function as a transcriptional switch, controlling Abeta cerebrovascular clearance and progression of AD.