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Validated All-in-One™ qPCR Primer for CD163(NM_004244.5) Search again
Product ID:
HQP022548
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
M130, MM130, SCARI1
Gene Description:
CD163 molecule
Target Gene Accession:
NM_004244.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- soluble CD163 inhibits phorbol ester-induced lymphocyte proliferation
- haptoglobin-dependent HbSR/CD163 scavenging system for hemoglobin clearance prevents toxic effects of hemoglobin in plasma and kidney
- has at least two distinct functions: the clearance of hemoglobin in its cell-bound form and participation in anti-inflammation as a soluble factor, exhibiting cytokine-like functions
- a metalloproteinase is responsible for LPS-mediated shedding of CD163
- CD163 mediates an anti-inflammatory pathway involving interleukin-10 release and heme oxygenase-1 synthesis.
- sCD163 may be a valuable laboratory parameter in monitoring diseases such as Gaucher.
- Increased numbers of CD163+ macrophages in SpA synovium and local production of sCD163 are associated with global inflammation as well as impairment of T cell activation, suggesting a dual role for CD163+ macrophages in Spondylarthropathy synovitis.
- SRCR domain 3 of CD163 is an exposed domain and a critical determinant for the calcium-sensitive coupling of haptoglobin.hemoglobin complexes
- sCD163-NMMHCA complexes were present in activated T lymphocytes after incubation with shed sCD163
- Serum levels of soluble CD163 were highly increased in reactive hemophagocytic syndrome, suggesting a macrophage-specific marker.
- in HIV encephalitis, expression of CD163 was far more widespread in grey matter ramified microglia than was expression of HLA-DR
- CD163 specifically reveals perivascular macrophages(PVM) in the normal human CNS. In MS lesions, CD163 staining reveals expression on foamy macrophages and microglia, besides an upregulation of the amount of PVM stained.
- results identify CD163 as a scavenger receptor for native Hb and small-molecular-weight Hb-based blood substitutes after Hp depletion
- These data suggest that hemoglobin may mediate a stimulatory effect on erythropoiesis through the activation of CD163 on hematopoietic progenitor cells.
- symptomatic plaques show a more pronounced induction of CD163 and HO-1 in response to plaque hemorrhages.
- only the beta-chain of haptoglobin is involved in CD163 receptor recognition
- The increased free-hemoblobin, haptoglobin, and sCD163 in chronic renal failure suggested 8-isoprostane-mediated suppression of Hb catabolism through CD163 receptor shedding
- CD163-mediated Hb-Hp uptake by peripheral blood monocytes constitutes an Hb-Hp clearance pathway, which acts at the site of intravascular hemolysis to reduce Hb-Hp circulation time and toxicity
- Individuals with diabetes mellitus and a haptoglobin 2.2 genotype demonstrate lower CD163 scavenger receptor levels and an impaired hemoglobin clearance capacity, with increased incidence of myocardial infarction.
- CD163 either acts as a TWEAK scavenger in pathological conditions or serves as an alternate receptor for TWEAK in cells lacking Fn14/TweakR.
- Activated macrophages are involved in ALF resulting in a 10-fold increase in sCD163. A high level (>26mg/l) of sCD163 was significantly correlated with fatal outcome and might be used with other parameters to determine prognosis.
- CD163 and HCP-1 constitute a linked pathway for Hb catabolism and heme-iron recycling in human macrophages.
- DC-sign+ CD163+ macrophages expressing hyaluronan receptor LYVE-1 are located within chorion villi of the placenta.
- High-level expression of CD163 is associated with leiomyosarcomas
- Data show that in cardiac surgical patients the expression of scavenger molecule CD163 on monocytes is significantly higher in "on-pump" patients than that of "off-pump" patients.
- studied gene expression profile of brain lesions of a patient with Neuromyelitis optica by using DNA microarray; found marked up-regulation of interferon gamma-inducible protein 30 (IFI30), CD163, and secreted phosphoprotein 1 (SPP1, osteopontin)
- Helpful for evaluation of a monocytic component in acute myeloid leukemias.
- Casein kinase II activity is increased by the binding of haptoglobin 1-1-hemoglobin to CD163.
- Data show that CD64 indexes for neutrophils and monocytes, and CD163 index for neutrophils can all be used for discrimination of SIRS and sepsis in critically ill neonates and children.
- during bacterial infection, CD163 on resident tissue macrophages acts as an innate immune sensor and inducer of local inflammation