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Validated All-in-One™ qPCR Primer for CD1D(NM_001766.3) Search again
Product ID:
HQP022129
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD1A, R3, R3G1
Gene Description:
CD1d molecule
Target Gene Accession:
NM_001766.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. [provided by RefSeq].
Gene References into function
- CD1d presents lipid and glycolipid antigens to T cells
- Data show that CD1d associates in the ER with both calnexin and calreticulin and with the thiol oxidoreductase ERp57 in a manner dependent on glucose trimming of its N-linked glycans.
- CD1d ligand at the human maternal-fetal interface
- B cell chronic lymphocytic leukemia cells significantly down-regulated transcripts from CD1c and CD1d genes, permitting cells to evade the immune response
- the CD1d alpha1-alpha2 domains of both rhesus monkeys and humans are highly homologous (95.6%)
- CD1d is expressed functionally on IECs with a polarity of presentation (basal > apical) predicting a role in presentation of mucosal glycolipid antigens to local CD1d-restricted T cells.
- a novel autocrine pathway of CD1d regulation by Hsp110.
- CD1d proteins sre strong binders of small hydrophobic probes such as 1-anilinonaphthalene-8-sulfonic acid and 4,4'-dianilino-1,1'-naphthyl-5,5'-disulfonic acid.
- saposins mobilize monomeric lipids from lysosomal membranes and facilitate their association with CD1d
- Investigation of the 5' upstream region of human CD1D reveals multiple transcription initiation sites and TATA boxless dual promoters located within 700 base pairs 5' upstream of the coding region.
- Transgenic overexpression of CD1d within pancreatic islets of nonobese diabetic (NOD) mice protects them from autoimmune diabetes through activation of NKT cells and improvement of IL-4 secretion at the site of autoimmunity (i.e., peripheral lymph nodes)
- CD1d can inhibit NK cell-mediated cytolysis; the putative inhibitory receptor does not recognize CD1d molecules loaded with alpha-GalCer
- Data show that phosphatidylinositol mannoside represents a mycobacterial antigen recognized by T cells in the context of CD1d.
- Hepatic inflammatory cells & biliary cells near portal tract fibrotic areas of HCV-infected donors specifically up-regulate CD1d. CD1d presentation of liver Ag may be beneficial in acute viral clearance, but in chronic infection could add to liver injury
- The ability of CD1d-unrestricted natural killer T cells to recruit innate immune system cells might play a role in cancer cell eradication and contribute to inflammatory diseases.
- TGFbeta produced by keratinocytes contribute to selectively downregulate CD1d expression on intraepidermal-resident Langerhans cells
- CD1d-dimer staining revealed human natural killer T cells reactivity toward Sphingomonas glycosphingolipids.
- ability of Nef to alter the cell surface expression of human CD1d. In cells co-expressing CD1d and Nef, a reduction in the cell surface level of CD1d was observed.
- analysis of the crystal structure of human CD1d in complex with synthetic alpha-galactosylceramide at a resolution of 3.0 A
- CD1d ligation alone, in the absence of iNKT, could rapidly (within 24 h) stimulate production of bioactive IL-12p70 by CD1d+ human peripheral blood monocytes as well as immature dendritic cells
- CD1d continues to play a role in late-stage NKT cell development and, in particular, during the functionally significant acquisition of NK1.1 that is indicative of NKT cell maturity
- CD1d has a role in intrahepatic T-cell recognition in hepatocytes
- Time-course studies of CD1d gene expression indicated that keratinocytes slowly increased gene expression with CaCl(2)-induced terminal differentiation
- These results highlight the variation in Ag recognition among CD1d-restricted Receptors, Antigen, T-Cell (TCRs) and suggest that TCR alpha-chain elements contribute to alpha-linked glycosphingolipid specificity [CD1d]
- CD1d-restricted gamma delta T cells specific for phospholipids can represent a key mucosal regulatory subset for the control of early host reactivity against tree pollens.
- This review highlights the role of the CD1d antigen processing pathway and the immunopotentiating effects of the ligands that can be presented by CD1d to natural killer (NK)T cells or other CD1d-restricted T cells during cancer and infections.
- Schwann cells activated iNKT cells in a CD1d-dependent manner in the presence of alpha-galactosylceramide
- CD1d has a role in cytolysis of lymphoblastic lymphoma cells
- One of the N-linked glycans (at position asparagine-42) exists mainly in a form that is sensitive to endoglycosidase H. Deletion of Asn-42 affects stability of the CD1d heavy chain beta 2-microglobulin heterodimer.
- CD4 potentiates human iNKT cell activation by engaging CD1d molecules. These results indicate that the CD4 coreceptors may contribute to the fine tuning of iNKT cells reactivity.
- saposin B may facilitate lipid binding to CD1d molecules throughout the endocytic pathway
- Cotrafficking with major histocompatibility class II molecules and the invariant chain (Ii) selectively enhances CD1d-mediated presentation of exogenous antigens.
- CD1d-restricted NKT cells have roles in the intestine and in inflammatory bowel diseases [review]
- The structure provides a basis for the interaction between the highly conserved NKT TCR alpha-chain and the CD1d-antigen complex
- These results indicate that CD4 can contribute to natural killer cell activation independently of the presence of a CD4-ligand on antigen presenting cells and suggest that it preferentially modulates cytokine and proliferative responses.
- Used a lentiviral system to generate stable cell lines producing beta2m-CD1d single chain protein which was used to form CD1d tetramer.
- Data indicate that viral danger signals trigger NKT cell activation by enhancing CD1d de novo synthesis through increasing the abundance of CD1D mRNA in human myeloid dendritic cells.
- monocyte-derived DCs cultured in an immunoglobulin-rich milieu expressed CD1d but not CD1a, CD1b, and CD1c, whereas DCs cultured in the presence of low levels of immunoglobulins had an opposite CD1 profile
- An alanine scanning mutagenesis approach was undertook to define the energetic basis of this interaction between the natural killer cell T cell receptors and CD1d.
- PKCzeta is an important transduction molecule downstream of TNF-alpha signaling and is associated with increased expression of CD1d that may enhance CD1d-natural killer T cell interactions in psoriasis lesions.
- Trophoblast differentiation is characterized by TGF-beta1-mediated decreases in trophoblast cell CD1d expression.
- CD1D expression and ligand induced cytokine production in mucosal epithelial cells from lower reproductive tract is tissue specific and CD1d-mediated cytokine production in penile urethral cells was abrogated by C. trachomatis infection.
- a distinct population of human CD1d-restricted T cells specific for inflammation-associated lysolipids suggest a novel mechanism for inflammation mediated immune regulation in human cancer
- Protection from experimental autoimmune encephalomyelitis afforded by the enrichment of natural killer T cells is independent of extrathymic CD1d transgene expression.
- Although the allogeneic activation of iNKT cells is invariant TCR-CD1d interaction-dependent, glycosphingolipid (GSL) profiling suggests it does not involve the recognition of disparate CD1d/GSL complexes
- CD1d overlaps with Ig-like transcript 4 (ILT4) both at the cell surface and in the cytoplasm; its interaction with ILT4 provides insights into the regulation of natural killer T cell-mediated immunity.