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Validated All-in-One™ qPCR Primer for LATS1(NM_004690.3) Search again
Product ID:
HQP022099
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
WARTS, wts
Gene Description:
large tumor suppressor kinase 1
Target Gene Accession:
NM_004690.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis.
Gene References into function
- regulation of function on the mitosis apparatus by Cdc/cyclin B
- molecular alterations found in soft tissue sarcoma
- kpm negatively regulates cell growth by inducing G(2)/M arrest and apoptotic cell death through its kinase activity
- WARTS plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G(1) tetraploidy checkpoint
- LATS1 is a novel cytoskeleton regulator that affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1.
- Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2
- Hypermethylation of the promoter region of LATS1 likely plays an important role in the down-regulation of LATS1 mRNA level in breast cancers, and breast cancers with a decreased expression of LATS1 mRNA level have a biologically aggressive phenotype.
- Activation of Omi / HtrA2-mediated cell death is a potential mechanism for the tumor suppressive activity of WARTS.
- Moderate overexpression of human LATS1 in cells exposed to microtubule poisons facilitated mitotic exit, and this activity required MOB1A.
- LATS1 and LATS2 mRNA was down-regulated in astrocytoma by hypermethylation of the promoter
- LATS1 inactivates YAP oncogenic function by suppressing its transcription regulation of cellular genes via sequestration of YAP in the cytoplasm after phosphorylation of YAP.
- Expression profiling of genes induced by ectopic expression of YAP or by knockdown of LATS1 reveals a subset of the potential Drosophila Hippo pathway targets implicated in epithelial-to-mesenchymal transition.
- Overexpression of YAP2 in cells promoted apoptosis, whereas the Mst2/Lats1-induced phosphorylation of YAP partially rescued the cells from apoptotic death.