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Validated All-in-One™ qPCR Primer for TRPA1(NM_007332.2) Search again
Product ID:
HQP021818
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ANKTM1, FEPS, FEPS1, p120
Gene Description:
transient receptor potential cation channel subfamily A member 1
Target Gene Accession:
NM_007332.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq].
Gene References into function
- cellular and molecular target for the pungent action of mustard oils; support an emerging role for TRP channel ANKTM1 as an ionotropic cannabinoid receptor
- TRPA1 is a novel substrate for the de-ubiquitinating activity of CYLD, and this de-ubiquitination has the net effect of increasing the cellular pool of TRPA1 proteins.
- Thiol reactive compounds of diverse structure activate TRPA1 in a manner that relies on covalent modification of cysteine residues within the cytoplasmic N terminus of the channel.
- TRPA1 is well conserved across the animal kingdom, with likely orthologs from human to nematode, which suggest an ancestral role for this channel, probably in sensation--{REVIEW}
- TRPA1 is activated by the insecticide and natural toxin allyl isothiocyanate which is known as insecticide.
- trypsin or tryptase released in response to tissue inflammation might trigger the sensation of pain by TRPA1 activation
- TRPA1 in the bladder epithelium might be involved in the bladder sensory transduction and the induction process of overactive bladder by BOO.
- While TRPA1 localisation is more widespread than TRPV1, it represents a promising novel drug target for the treatment of chronic pain and hypersensitivity.
- Our findings reveal a molecular basis of species-specific channel gating and provide novel insights into how TRPA1 respond to stimuli.
- The results of this study support the hypothesis that 4-ONE is a relevant endogenous activator of vagal C-fibres via an interaction with TRPA1, and at less relevant concentrations, it may activate nerves via TRPV1.
- TRPA1 is required for sensitivity of cultured dorsal root ganglion neurons to 15-deoxy-Delta12,14-prostaglandin J2 (15d-PGJ2).
- TRPA1 channels are targeted by an array of inflammatory mediators to elicit inflammatory pain in the nervous system
- TRPA1 is potentiated and inactivated by permeating calcium ions
- TRPA1 plays an important role in the mechanisms responsible for mechanical hypersensitivity observed in inflammatory and neuropathic pain models
- Caffeine does not activate human TRPA1; instead, it suppresses its activity.
- New studies have revealed an essential role for TRPA1, a sensory neuronal TRP ion channel, in airway chemosensation and inflammation--{REVIEW}
- Pore dilation occurs in TRPA1, but not in TRPM8 channels.