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Validated All-in-One™ qPCR Primer for SPHK1(NM_182965.2) Search again
Product ID:
HQP021659
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
SPHK
Gene Description:
sphingosine kinase 1
Target Gene Accession:
NM_182965.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Summary
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions.
Gene References into function
- These findings show a role for SphK in the signal transduction by TRAF2 specifically leading to activation of NF-kappa B and antiapoptosis.
- synthesize sphingosine 1-phosphate,a lipid mediator in intracellular signaling system
- results indicate that SPHK1-interacting protein(SKIP) is a novel protein likely to play a regulatory role in the modulation of SPHK1 activity
- activation and translocation to cell membrane dependent on protein kinase C
- characterization of the nucleotide-binding site of human sphingosine kinase 1 through a combination of site-directed mutagenesis and affinity labeling with the ATP analogue, FSBA
- role for sphingosine kinase in the regulation of neutrophil priming
- Resting tone and myogenic responses of isolated hamster resistance arteries increased with forced expression of human Sphk1 in smooth muscle cells
- Activation of this enzyme in mediated by ERK1/2 phoshporylation
- results provide the first evidence of the active nuclear export of SPHK1 and suggest it is mediated by a CRM1-dependent pathway
- Data show that respiratory syncytial virus stimulates sphingosine kinase activity in lung epithelial cells, leading to activation of antiapoptotic sphingosine 1-phosphate and subsequently activation of Akt and extracellular signal-related kinase.
- SK1 is down-regulated by genotoxic stress, and basal SK1 function may be necessary for the maintenance of tumor cell growth
- hereditary neuralgic amyotrophy is not caused by point mutations of sphingosine kinase 1
- SphK1 mediates TNF-alpha-induced MCP-1 gene expression through a p38 MAPK-dependent pathway and may participate in oscillatory flow-mediated proinflammatory signaling pathway in the vasculature.
- PI3K and ERK/MAPK mediated the activation of sphingosine kinase and would be involved in the HGF-induced migration of endothelial cells.
- SPHK1 not only plays a key role in the generation and release of proinflammatory mediators triggered by anaphylatoxins from human macrophages but is also involved in the process of immune cell motility.
- SPHK plays an important role in the immune-inflammatory pathologies triggered by anaphylatoxins in human neutrophils
- Comparison of base sequence of SPHK1/Sphk1 in human, mouse, and rat.
- role for SK in the regulation of vascular phenomena that occur under conditions of stress
- SK1 has marked effects on cell function, with plasma membrane-associated SK having a potent inhibitory effect on the G1-S phase transition [SK1]
- SK1 down-regulation by TNF is dependent on the lysosomal pathway of apoptosis and specifically on cathepsin B, which functions as an SK1 protease in cells
- model in which M.tuberc. inhibits both the activation and phagosomal translocation of sphingosine kinase 1 to block the localized Ca2+ transients required for phagosome maturation
- IL-1beta and TNF-alpha induced an early and sustained increase in SPHK activity in INS-1 cells and isolated islets.
- Findings demonstrate a role of SPHK1 activation in proinflammatory signalling and of SPHK1 basal activity in survival of A549 lung carcinoma cells.
- results suggest that SphK1 may be critical for growth, metastasis and chemoresistance of human breast cancers
- the specific plasma membrane localization and activation of SK1 is mediated largely by specific lipid-protein interactions
- For the first time endogenous SK1 is implicated as an important survival enzyme in MCF-7 cells. The biological consequences of knocking down the enzyme are linked to its biochemical role as a regulator of sphingolipid metabolism.
- These findings strongly suggest that high expression and up-regulation of SPHK1 and S1P receptors protect PC3 cells from the apoptosis induced by CPT.
- These findings uncover a new functional role for Sphingosine kinase 1 (SK1), which can control survival/death balance by targeting sphingolipid de novo biosynthesis.
- Data show that protein kinase C-alpha activation in endothelial cells leads to upregulation of sphingosine kinase-1 expression and activity, which is critically involved in the mechanism of endothelial cell migration.
- Data shows that sphingosine kinase 1 (SPHK1) is the isoform activated by TNFalpha. It plays an essential role on intracellular Ca2+ signals, degranulation, cytokine production, activation of NFkappaB, and a proinflammatory response triggered by TNFalpha.
- results suggest that export of Sphk-1a isoform occurs under physiological conditions and may contribute to the establishment of the vascular S1P gradient.
- SK1 is cleaved by cathepsin B in a sequential manner after basic amino acids, and the initial cleavages at the two identified sites, histidine 122 and arginine 199, occur independently of each other.
- SphK-dependent Akt activation plays a significant role in TNF-alpha-induced cyclin D expression and cell proliferation
- IGFBP-3 induces angiogenesis through IGF-I- and SphK1-dependent mechanisms
- the PI3K/AKT/mTOR signaling pathway is involved in regulation of SphK1, with AKT2 playing a key role in PDGF-induced SphK1 expression
- the localization and activity of SK1 are coordinately regulated with actin dynamics during macrophage activation
- BCR/ABL induces SPK1 expression and increases its cellular activity, leading to upregulation of Mcl-1 in CML cells.
- These data suggested that SPHK1 activation by TGF-beta1 leads to Rho-associated myofibroblasts differentiation mediated by transactivated S1P receptors in the lung fibrogenic process.
- the SKase pathway, through the generation of S1P, is critically involved in mediating globular adiponectin-induced endothelial cell activation
- High expression of sphingosine kinase 1 is associated with estrogen receptor negative breast breast cancer.
- These data suggest that differential formation of sphingosine-1-phosphate by SphK1 and SphK2 has distinct and important actions in human mast cells.
- Activation of sphingosine kinase-1 mediates induction of endothelial cell proliferation and angiogenesis by epoxyeicosatrienoic acids.
- Ang-1-induced inhibition of leakiness is mediated through activation of SK-1, defining a new signaling pathway in the Ang-1 regulation of permeability.
- The hypoxia-stimulated SK-1 upregulation was functionally coupled to increased migration since the selective depletion of SK-1, but not of SK-2, by siRNAs abolished the migratory response.
- a novel mechanism of regulation of both SK1 and SK2 that is mediated by their interaction with eEF1A.
- apoptotic cells may up-regulate SphK1 to produce and secrete sphingosine-1-phosphate that serves as a "come-and-get-me" signal for scavenger cells to engulf them in order to prevent necrosis
- SphK1 is inolved in regulating imatinib-induced apoptosis and SphK1 is a downstream effector of the Bcr-Abl/Ras/ERK pathway inhibited by imatinib but upstream regulator of Bcl-2 family members
- Sphingosine kinases and sphingosine-1-phosphate are critical for transforming growth factor beta-induced ERK1 and ERK2 activation and promotion of migration and invasion of esophageal cancer cells
- Datas suggest that filamin A links sphingosine kinase 1 and sphingosine-1-phosphate receptor 1 to locally influence the dynamics of actin cytoskeletal structures at lamellipodia to promote cell movement.
- SphK1/sphingosine-1-phosphate pathway contributes to colon carcinogenesis
- the crucial role of activation of the SPHK1S1PS1P1 signaling pathway in response to inflammatory mediators in endothelial cells in regulating endothelial barrier homeostasis.
- PP2A appears to function as an endogenous regulator of SK1 phosphorylation
- Sphingosine kinase 1 is a new modulator of hypoxia inducible factor 1alpha during hypoxia in human cancer cells.
- SPHK1 in astrocytoma cell lines was elevated at both mRNA and protein levels, and the SPHK1 mRNA and protein were significantly up-regulated by up to 6.8- and 40-fold, respectively, in primary astrocytomas
- Anti-inflammatory effects of sphingosine kinase modulation in inflammatory arthritis