|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for KAT2B(NM_003884.4) Search again
Product ID:
HQP021621
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CAF, P/CAF, PCAF
Gene Description:
lysine acetyltransferase 2B
Target Gene Accession:
NM_003884.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq].
Gene References into function
- P/CAF HAT activity and induction of ap97 are involved in calcium-dependent keratinocyte differentiation
- Transcriptional synergy between Tat and PCAF is dependent on the binding of acetylated Tat to the PCAF bromodomain
- acetylated IRF7 displayed impaired DNA binding capability and that over-expression of PCAF led to decreased IRF7 activity
- Data suggest replication stimulation occurs through recruitment of large T antigen to the origin and acetylation by PCAF or GCN5.
- PCAF binding to HIV-1 tat is regulated by the differential acetylation of tat
- HPV 6, 16 and 18 E7 proteins interact with the pCAF acetyltransferase. HPV 16 E7 interacts with the acetyltransferase domain of pCAF, and reduces its acetyltransferase activity in vitro.
- Mechanisms of P/CAF auto-acetylation.
- ER81 is acetylated by two coactivators/acetyltransferases, p300 and p300- and CBP-associated factor (P/CAF) . Whereas p300 acetylates two lysine residues (K33 and K116) within the ER81 N-terminal transactivation domain, P/CAF targets only K116.
- results demonstrate for the first time a novel role for human proliferating cell nuclear antigen(PCNA)in transcriptional repression and in modulating chromatin modification with reciprocal modulation of p300 acetyltransferase and PCNA by each other
- PCAF stimulates p73-mediated transactivation
- acetyltransferase p300 and the receptor tyrosine kinase HER2/Neu activate protein ER81
- acetylation of AML1 through p300 is a critical manner of posttranslational modification and identify a novel mechanism for regulating the function of AML1
- the stability of PCAF is regulated by MDM2 by its ubiquitination and degradation
- P/CAF and p300 have roles in acetylation-induced stabilization of E2F1
- PCAF expression can be induced by wild-type p53
- stabilization of NF-E4 by acetylation is PCAF-dependent; acetylation of Lys(43) also reduces the interaction between NF-E4 and HDAC1, potentially maximizing the activating ability of the factor
- kinetic occupancy of p300 at mitogen-induced genes in activated T cells reveals promoters that share common patterns of inducible expression, p300 recruitment, dependence on selective p300 domains, and sensitivity to histone deacetylase inhibitors.
- p300 is involved in the transcriptional regulation of IL-12 p40, and IL-12 p40 is one of the target genes of p300
- GCN5 acetylates c-myc oncoprotein and regulates its function by altering its rate of degradation.
- The HIV Tat protein can be endocytosed by cells and interacts with PCAF, inducing neuronal apoptosis.
- transforming growth factor beta type II receptor promoter activity and acetylation of Sp1 by recruitment of PCAF/p300 to a Sp1.NF-Y complex are induced by Trichostatin A
- CBP and PCAF coactivators mediate ERK1 gene expression at both the transcriptional and post-transcriptional level
- the SREBP-1c.BETA2.E47 complex is in a DNA looping structure which is required for efficient recruitment of CREB-binding protein/p300
- A genetically engineered PCAF protein exhibits catalytic parameters within 3-fold of those of the wild-type PCAF catalytic domain, suggesting that the loop mutation was not deleterious for HAT activity.
- p300 acetylates of three specific lysines (K264, K266, K273) in the carboxy-terminus of IN, a region that is required for DNA binding.
- DEK interacts with histones and exerts a potent inhibitory effect on both p300 and PCAF-mediated histone acetyltransferase activity and transcription.
- p300 acts in early G1 to prevent RB hyperphosphorylation and delay premature S-phase entry
- Overexpression of p300/Creb binding protein-associated factor increases both the promoter reporter expression and endogenous mRNA level of erythroid-specific 5-aminolevulinate synthase (ALAS2).
- p72 RNA helicase may not only be involved in the p53-Mdm2 regulatory loop, but also profoundly impact on the transcriptome through various CBP/p300 and P/CAF interacting proteins.
- PCAF, in addition to its acetyltransferase activity, possesses an intrinsic ubiquitination activity that is critical for controlling Hdm2 expression levels, and thus p53 functions.
- WRN is a novel cellular cofactor for HIV-1 replication, and the WRN helicase participates in the recruitment of PCAF/P-TEFb-containing transcription complexes
- Both PCAF and BRG1 are also involved in the activation of the myogenin gene in rhabdomyosarcoma
- In vivo co-immunoprecipitation assays showed that p73gamma interacted preferentially with PCAF.
- a large multiprotein complex, which includes Fra-1, p300, P/CAF, junD, junB, and Sp1 acts at the AP1-5 site to produce a synergistic increase in hINV gene expression
- PCAF-dependent acetylation of lysine 380 abrogates repressor function of Fli1 with respect to collagen expression; TGFb-dependent acetylation of Fli1 may represent the principal mechanism for TGFb-induced dissociation of Fli1 from the collagen promoter
- KLF4 might function as an activator or repressor of transcription depending on whether it interacts with co-activators such as p300 and CREB-binding protein or co-repressors such as HDAC3.
- a novel regulatory role of p38 during neuroinflammation where this MAP kinase controls acetylation of NF-kappaB p65 by regulating acetyltransferase activity of coactivator p300.
- histone acetyltransferase P/CAF is a promiscuous histone propionyltransferase
- P/CAF regulates CDK9 function by specifically acetylating the catalytic core of the enzyme especially a Lys needed for ATP coordination & the phosphotransfer reaction.
- Three-dimensional solution structures of the bromodomains of p300/CBP-associated factor (PCAF) bound to peptides derived from histone acetylation sites.
- Phosphorylation deficient c-jun was less able to recruit PCAF to AP-1 sites.
- Recent data indicate that alterations in the expression and/or activity of androgen receptor coactivator proteins occur during PC progression that can foster androgen depletion independent activation of the androgen receptor.
- PCAF regulates the balance between cell-cycle arrest and apoptosis in hypoxia by modulating the activity and protein stability of both p53 and HIF-1alpha.
- An interaction between the human T cell leukemia virus type 1 basic leucine zipper factor (HBZ) and the KIX domain of p300/CBP contributes to the down-regulation of tax-dependent viral transcription by HBZ.
- Here it is shown that Zac1, together with the coactivators p300 and PCAF, recruit to the p21(Cip1) promoter during the differentiation of embryonic stem cells into neurons.
- Small increments in p300 are necessary and sufficient to drive myocardial hypertrophy, possibly through acetylation of MEF2 and upstream of signals promoting phosphorylation or nuclear export of histone deacetylases
- The histone acetyltransferase (HAT) PCAF associates with actin and hnRNP U.
- STAT3 NH(2)-terminal domain plays an important role in the interleukin-6 signaling pathway by interacting with the p300 bromodomain, thereby stabilizing enhanceosome assembly.
- A critical and selective role of p/CAF in PTF1-dependent gene activation during acinar differentiation.
- ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein
- bexarotene increased the occupancy of the identified enhancer element in IGFBP-6 gene by RXRalpha, RARbeta, cJun, cFos, and p300
- P/CAF deacetylation by HDAC3 and in a minor degree by HDAC1, HDAC2, or HDAC4 leads to cytoplasmic accumulation of P/CAF.
- These results indicate that p/CAF is a novel HOXA10 target gene, and HOXA10 promotes human endometrial development, at least in part, through the regulation of p/CAF gene.
- The phosphorylation-acetylation cascade triggered by PKC delta represents the primary mechanism whereby TGF-beta regulates the transcriptional activity of Fli1 in the context of the collagen promoter.