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Validated All-in-One™ qPCR Primer for TNKS(NM_003747.2) Search again
Product ID:
HQP021405
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ARTD5, PARP-5a, PARP5A, PARPL, TIN1, TINF1, TNKS1, pART5
Gene Description:
tankyrase
Target Gene Accession:
NM_003747.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- The telomeric poly(ADP-ribose) polymerase, tankyrase 1, contains multiple binding sites for telomeric repeat binding factor 1 (TRF1) and a novel acceptor, 182-kDa tankyrase-binding protein (TAB182).
- Identification of a binding motif shared by IRAP TAB182, and human TRF1 but not mouse TRF1
- Results describe the expression and enzymatic characterization of full-length tankyrase 1 and a fragment, termed T-PARP, spanning the poly(ADP-ribose) polymerase domain.
- Tankyrase 1 interacts with Mcl-1 proteins and inhibits their regulation of apoptosis
- co-immunoprecipitation of endogenous proteins in 293T cells confirms physiological relevance of TNKS as interaction partner of FBP17
- Results provide evidence that specific ankyrin repeat cluster-TRF1 interactions play roles in the essential catalytic function of tankyrase 1.
- telomeres may require a unique tankyrase 1-dependent mechanism for sister chromatid resolution before anaphase
- TIN2 protected TRF1 from poly(ADP-ribosyl)ation by tankyrase 1 without affecting tankyrase 1 automodification.
- TNKS1 bound to Epstein-Barr virus origin of plasmid replication in coimmunoprecipitation experiments with transfected cell lysates.
- The role of TNKS in the poly(ADP-ribosyl)ation of the mitotic spindle apparatus is reported.
- tankyrase-1 appears to protect cells by preventing genotoxins from activating PARP 1-mediated reactions such as PARP 1 automodification and NAD(+) consumption
- Cell-cell contact makes tankyrase associate with the lateral membrane. PARsylating activity causes translocation to cytosol, ubiquitination & proteasomal degradation. Recruitment to this site suggests a role in polarized protein targeting in epithelium.
- tankyrase1 is a poly(ADP-ribose) polymerase with roles in telomere length control by the TRF1 component of the shelterin complex
- TNKL and TNKS are aberrantly expressed in colon tumors and contain multiple epitopes that induce humoral and cellular immune responses in cancer patients
- The structure of the catalytic poly(ADP-ribose) polymerase (PARP) domain of human tankyrase 1 is reported.
- This paper suggests a novel candidate gene for Cornelia de Lange syndrome - tankyrase 1.