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Validated All-in-One™ qPCR Primer for AOC3(NM_003734.3) Search again
Product ID:
HQP021378
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HPAO, SSAO, VAP-1, VAP1
Gene Description:
amine oxidase copper containing 3
Target Gene Accession:
NM_003734.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor.
Gene References into function
- VAP-1 is expressed on hepatic sinusoidal endothelial cells in vitro and supports adhesion and transmigration of lymphocytes across these cells under physiological shear stress.
- Data show that increases in vascular adhesion protein-1 (VAP-1) due to absolute or relative insulin deficiency may be directly involved in the pathogenesis of diabetic angiopathy.
- normal mice and human lungs revealed vascular adhesion protein-1 expression in the endothelium of large and mid-sized pulmonary vessels
- may convert endogenous amines, like methylamine, to vasoactive metabolites.
- the oxidase activity of VAP-1 controls PMN exit from the blood during the relatively poorly understood transmigration step
- Semicarbazide-sensitive amine oxidase is a source of oxidative stress in diseased human skeletal muscle; it contributes to oxidative stress-induced damage in various inflammatory and other myopathies.
- VAP-1 protein expression is significantly decreased in intratumoral vessels compared with peritumoral ones; this difference was independent of the skin melanoma thickness
- Plasma semicarbazide-sensitive amine oxidase (SSAO) is elevated in patients with type 1 and type 2 diabetes and has been implicated in the pathophysiology of diabetic late complications.
- results suggest that vascular adhesion protein-1 (VAP-1) levels are correlated with fasting glucose and insulin in morbidly obese subjects, and after surgery VAP-1 levels were associated with changes in visceral adiposity and diastolic blood pressure
- The dimer structure reveals intriguing features that may have fundamental roles in the adhesive and enzymatic functions of hVAP-1.
- This study indicates the important role of VAP-1 in the pathogenesis of chronic inflammatory cutaneous disorders, including AE.
- A role for AOC3-mediated deamination in pulmonary inflammation was shown.
- Tetragonal crystals were obtained and a data set extending to 2.5 A was collected. The crystals are merohedrally twinned and the estimate of the twinning fraction was complicated by pseudo-symmetry of the crystals.
- VAP-1 may contribute critically to the oxidase activities in utero, and prove important for lymphocyte trafficking during human ontogeny.
- We propose that VAP-1 might participate in controlling leukocyte entry into inflamed brain.
- Expression of VAP-1, Stabilin-1, L-SIGN can be used to identify sinusoidal endothelium and to permit discrimination from vascular and lymphatic endothelial cells.
- We propose that as well as directly promoting adhesion via interactions with the as yet unknown ligand, binding of enzyme substrate to VAP-1 can indirectly promote inflammatory cell recruitment via upregulation of adhesion molecules and chemokines.
- Taken together, these results allow us to postulate that SSAO may contribute to the vascular damage associated to AD.
- VAP-1 was located solely in the membrane fraction of the vascular smooth muscle cell. However it was also shown to be released into the cell-culture medium.
- semicarbazide-sensitive amine oxidase expression during in vitro differentiation of human preadipocytes and in adipose and stroma-vascular fractions of human fat depots
- The elevated serum SSAO activity measured through the detection of the enzyme-generated hydrogen peroxide in HD patients might indicate its contribution to the accelerated atherosclerotic disease observed in uremia.
- Data suggest atherosclerotic inflammation may be a trigger for sclerosis in calcified stenotic aortic valves through upregulation TGF-beta, VAP-1, MIG and Eotaxin3, which is only partially inhibited by previous statin therapy.
- VAP-1 may aggravate ischaemic vascular changes and thhe subsequent release of VAP-1 into circulation could be further examined as a potential marker of early ischaemic vasculopathy.
- Serum levels of VAP-1 are associated with the severity of kidney damage or stages of kidney disease.
- VAP-1 regulates the inflammatory response in ischemia-reperfusion injury and suggest that blockade of VAP-1 may have therapeutic value.