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Validated All-in-One™ qPCR Primer for BHLHE40(NM_003670.2) Search again
Product ID:
HQP021270
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BHLHB2, Clast5, DEC1, HLHB2, SHARP-2, SHARP2, STRA13, Stra14
Gene Description:
basic helix-loop-helix family member e40
Target Gene Accession:
NM_003670.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. The encoded protein is believed to be involved in the control of cell differentiation.
Gene References into function
- hypoxia-induced gene in pancreatic cancer cell lines
- DEC1-mediated anti-apoptosis is achieved by blocking apoptotic pathways initiated via the mitochondria. The results functionally distinguish DEC1 from other bHLH proteins and directly link this factor to oncogenesis.
- DEC1 and DEC2 may play a crucial role in the adaptation to hypoxia
- DEC1 is the first transcription factor that can promote both chondrogenic differentiation and terminal differentiation
- Dec1 and Dec2 are regulators of the mammalian molecular clock, and form a fifth clock-gene family.
- DEC1-mediated repression on the expression of DEC2 provides an important mechanism that these transcription factors regulate the cellular function of members within the same class
- Findings suggest that the basic region of DEC1 participates in the transcriptional regulation through a protein-protein interaction with BMAL1 and DNA binding to the E-box.
- These findings suggest that Dec1 modulates osteogenic differentiation of mesenchymal stem cells by inducing the expression of several, but not all, bone-related genes.
- link between HIF-1 and STAT1 reveals a previously unknown role of STRA13 in hypoxia and carcinogenesis
- A multi-locus interaction between rs6442925 in the 5' upstream of BHLHB2, rs1534891 in CSNK1E, and rs534654 near the 3' end of the CLOCK gene, however, is significantly associated with bipolar disorder