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Validated All-in-One™ qPCR Primer for PARG(NM_003631.2) Search again
Product ID:
HQP021129
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
PARG99
Gene Description:
poly(ADP-ribose) glycohydrolase
Target Gene Accession:
NM_003631.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. [provided by RefSeq].
Gene References into function
- Subcellular localization of human poly(ADP-ribose) glycohydrolase in mammalian cells.
- PARG is expressed in alternatiuve splice variants yielding isoforms that localize to different cell compartments.
- PARG in modulating chromatin structure, participares in transcription, DNA repair and apoptosis [review].
- PARG is resident in FMRP (Fragile-X mental retardation protein)-associated messenger ribonucleoparticles complexes.
- results suggest that PARG may play an autonomous role in the cellular response to oxidative DNA damage
- hPARG55, that can be expressed through alternative translation initiation from hPARG60 transcripts is strictly targeted to the mitochondria.
- PARG is identified as a novel and critical component of single-strand break repair that accelerates this process in concert with PARP-1.
- PPAR gamma ligands inhibit growth and induce apoptosis of liver cancer cell