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Validated All-in-One™ qPCR Primer for EEA1(NM_003566.3) Search again
Product ID:
HQP020493
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
MST105, MSTP105, ZFYVE2
Gene Description:
early endosome antigen 1
Target Gene Accession:
NM_003566.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- activation of p38 MAPK caused a decrease in EEA1 colocalization with phagosomes, arresting maturation into the phagolysosome
- macropinosomes do not mature to late endosomes or fuse with lysosomes. EEA1 continuously mediates homotypic fusion as long as the macropinosomes persist.
- These results suggest that EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
- Results describe the geometry of the micelle penetration of the early endosome antigen 1 FYVE domain.
- PIKfyve is distributed in microdomains that are distinct from those occupied by EEA1 and Hrs
- Impairment of internalization of specific glutamate receptors and their subsequent accumulation in the synapse may account for the neurological deficits observed in some patients developing EEA1 autoantibodies.
- results suggest that the local production of PtdIns(3)P implicates the fusion of macropinosomes via EEA1 as well as conventional early endosomes