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Validated All-in-One™ qPCR Primer for AXIN2(NM_004655.2) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
Gene References into function
- Activation of AXIN2 expression by beta-catenin-T cell factor
- Mutations in AXIN2 cause familial tooth agenesis and predispose to colorectal cancer
- axin2 expression is regulated by its alternative 5'UTR mRNA
- E2F1 induces stabilisation of axin2 mRNAs
- epigenetic silencing of AXIN2 is specifically associated with carcinogenesis in colorectal carcinoma with microsatellite instability
- Results show Low frequency of AXIN2 mutations in patients with multiple polyposis.
- The identification of a beta-catenin-T-cell factor-regulated Axin2-GSK3beta-Snail1 axis provides new mechanistic insights into cancer-associated epithelial-mesenchymal transition programmes.
- AXIN1, AXIN2 and TCF7L2 may have roles in development of colorectal carcinomas [review]
- Findings indicate that mutations of AXIN2 can lead to an oncogenic activation of the Wnt pathway in medulloblastomas.
- Mutations in AXIN1 and AXIN2 may contribute to gastric carcinogenesis.
- a high level of p53 downregulates the beta-catenin expression, but this effect is attenuated by non-functional AXIN2 or betaTrCP in lung cancer.
- Germline AXIN2 mutation is associated with melanoma
- Five snps in AXIN2 were associated with increased breast cancer risk. AXIN2 rs4791171 was significantly associated with risk in premenopausal women.
- Frameshift mutations of Wnt pathway genes AXIN2 and TCF4 in gastric carcinomas with high microsatellite instability are reported.
- The rs8081536 allelic variation in AXIN2 gene does not contribute to the susceptibility of HSCR in the patients. AXIN2 rs2240308 and rs9913621 allelic variation might be related to HSCR.
- a gene that when mutated increases susceptibility to colon cancer also is associated with cleft lip and palate