|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for NCOA3(NM_006534.3) Search again
Product ID:
HQP020040
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ACTR, AIB-1, AIB1, CAGH16, CTG26, KAT13B, RAC3, SRC-3, SRC3, TNRC14, TNRC16, TRAM-1, bHLHe42, pCIP
Gene Description:
nuclear receptor coactivator 3
Target Gene Accession:
NM_006534.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
Gene References into function
- CAG/CAA repeat length in AIB1/SRC-3 gene may be associated with prosate cancer risk in Chinese men
- the effect of STAT5b-RARalpha on the activity of myeloid transcription factors including STAT3, and STAT5 as well as its molecular interactions with the nuclear receptor corepressor, SMRT, and nuclear receptor coactivator, TRAM-1.
- Results show that IKK-mediated phosphorylation of SRC-3 is a regulated event that plays an important role and also substantiate the role of SRC-3 in multiple signaling pathways.
- SRC-3 has a role in the vasoprotective effects of estrogen and in cell migration, proliferation and cancers [review]
- likely prognostic indicator for patients with breast tumors
- AIB1 nuclear expression correlated with estrogen receptor alpha status in breast cancer; patients with AIB1 nuclear expression tended to be successfully treated by hormonal therapy
- Polyglutamine repeat length in the AIB1 gene modifies breast cancer susceptibility in BRCA1 carriers
- The polymorphic polyglutamine tract of the AIB1 gene is somatically unstable in breast cancer tissue and cell lines.
- AIB1-Delta3 causes a significant increase in the efficacy of 17beta-estradiol at both estrogen receptor-alpha (ER-alpha) and ER-beta in ovarian, breast and endometrial cancer cell lines
- HER2/Neu and ACTR may synergize to orchestrate mammary tumorigenesis through the dysregulation of the transcription factor ER81 and its target genes
- results reveal an essential role of ACTR in control of breast cancer cell proliferation and implicate the ACTR-E2F1 pathway as a novel mechanism in antiestrogen resistance
- AIB1 plays a dual role in regulating ERalpha activity, one in recruiting transcription factors including other coactivators involved in gene activation and the other in regulating ERalpha protein degradation mediated by the ubiquitin-proteosome machinery
- AIB1 has a major role in the multistage progression of pancreatic cancer
- AIB1 is required for IGF-I-induced proliferation, signaling, cell survival, and gene expression in human breast cancer cells, independent of its role in estrogen receptor signaling
- the N-terminally deleted isoform of AIB1 can play a role in breast cancer development and/or progression
- The ability of SRC family coactivators to regulate the expression of one of these genes, PARD6B/Par6, was confirmed by using cells individually depleted of SRC-1.
- Single Nucleotide Polymorphisms in NCOA3 is associated with breast cancer risk
- There is no significant effect of AIB1 genetic variation on breast cancer risk in BRC1 or BRCA2 mutation carriers.
- SRC-3 phosphorylation occurs only when either activated estrogen receptor alpha (ERalpha) or activated ERbeta is present.
- SRC-3 coactivator protein-protein complex formation is modulated by Pin1.
- Since AIB1 appears to modulate effect of endogenous hormones via the estrogen receptor, and smoking affects circulating hormone levels, results support evidence that steroid hormones play important role in breast carcinogenesis in BRCA1 mutation carriers
- allelic variation at the NCOA3 locus may contribute to the genetic control of androgenic hormone and IGF levels and vertebral bone mass among older men
- These studies reveal a novel role for SRC-3 in ovarian cancer progression by promoting cell migration, independently of its role in estrogen receptor signaling.
- the AIB1 glutamine repeat does not substantially modify risk of breast cancer in BRCA1 and BRCA2 mutation carriers
- Findings suggest a potential role of SRC-3 in the control of esophageal squamous cell carcinomas (ESCC) cell proliferation; such may be responsible, at least in part, for tumorigenesis and/or progression of ESCC.
- ACTR has direct control of cell cycle gene expression; its autoregulation underlies its transforming activities.
- poly Q encoding region of AIB1 gene is somatic unstable in breast cancer cell line
- estrogen treatment led to increased phosphorylation and decreased sumoylation of AIB1; sumoylation coordinated with phosphorylation in regulating the transcriptional activity of AIB1
- There is a positive feedback regulatory loop consisting of E2F1 and SRC-3 to maintain high levels of SRC-3 and E2F1 activity, which may partially interpret the oncogenic role of SRC-3 overexpression.
- androgen receptor and ACTR may play important roles in androgen ablation resistance by controlling key cell cycle gene expression
- Results suggest that members of the SRC coactivator family, such as SRC-3, serve as substrates for the enzymatic coactivator coactivator-associated arginine methyltransferase 1 (CARM1).
- Data show that downregulation of AIB1 by Mir-17-5p resulted in decreased estrogen receptor-mediated, as well as estrogen receptor-independent, gene expression and decreased proliferation of breast cancer cells.
- Serum withdrawal or growth in high cell density caused rapid degradation of AIB1 protein, but not mRNA, in immortalized breast cancer cell lines.
- nuclear receptor coactivator-3 has a role in primary cutaneous melanoma
- p/CIP/SRC-3 activity and stability are regulated by CARM1-dependent methylation
- Both DRIP205 and SRC-3 are required for the keratinocyte differentiation
- the SRC3-myocardin interaction is a site of convergence for nuclear hormone receptor-mediated and VSMC-specific gene regulation
- SRC-3 might be an important indicator of uterine endometrial cancer advancement and survival.
- ubiquitination of SRC-3 is a phospho-mediated biphasic event and a transition from multi-(mono)ubiquitination (SRC-3 activation) to long-chain polyubiquitination (SRC-3 degradation) is processive during the coactivation of select transcription factors
- AIB1 was associated to disease-free survival for breast cancer patients treated with 2 years of adjuvant tamoxifen.
- Results indicate that phosphorylation coordinates SRC-3 coactivator function by linking the probabilistic formation of transient nuclear receptor-coactivator complexes with its molecular dynamics and cellular compartmentalization.
- MYST3-NCOA3 gene fusions were found in AML-M5. The breakpoint in NCOA3 is at bases 2752 & 2376 in the MYST3-NCOA3 & NCOA3-MYST3 fusion transcripts, respectively. The CREB-interacting domain is conserved.
- RAC3 is required for TRAIL resistance and this anti-apoptotic function is independent of its role in hormone receptor signaling.
- RAC3 has been found associated with a protein complex containing AIF, Hsp90 and dynein, suggesting a role for the co-activator in the cytoplasmatic nuclear transport of these proteins associated with cytoskeleton
- ACTR functions as a coactivator of AP-1 to up-regulate the expression of matrix metalloproteinases such as MMP-7 and MMP-10 and reduce cell adhesion to specific extracellular matrix proteins.
- AIB1 with an increased UC (Urothelial Carcinoma) labeling index of Ki-67 was observed. AIB1 is an independent molecular marker for poor prognosis (shortened survival time) of patients with Urothelial Carcinoma.
- Results show that protein kinase C zeta phosphorylates and specifically stabilizes SRC-3 in a selective ER-dependent manner.
- Precise control of the cellular concentration of SRC-3 may thus serve as a mechanism for defining growth responses to estrogen receptors and other growth-promoting transcription factors
- overexpression of AIB1 in nasopharyngeal carcinoma may be important in the acquisition of an invasive and/or metastatic phenotype.
- Dysregulation of nuclear shuttling and proteasomal degradation may modulate the oncogenic potential of AIB1.
- SRC-3/AIB1 plays an essential role in prostate cancer cell invasion and metastasis.
- In 560 human breast tumors, AIB1 expression was found to be positively associated with PEA3, MMP2, and MMP9
- These results demonstrate regulated tyrosine phosphorylation of AIB1/SRC-3 at a C-terminal tyrosine residue (Y1357) that is phosphorylated after insulin-like growth factor 1, epidermal growth factor, or estrogen treatment of breast cancer cells.
- AIB1 is frequently overexpressed in human breast cancer and has been shown to be associated with tamoxifen resistance
- The phosphatases PDXP, PP1, and PP2A were identified to be key negative regulators of SRC-3 transcriptional coregulatory activity in steroid receptor signalings.
- role of AIB1 in oestrogen receptor -growth factor interactions.