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Validated All-in-One™ qPCR Primer for HMGA2(NM_003483.4) Search again
Product ID:
HQP019812
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BABL, HMGI-C, HMGIC, LIPO, SRS5, STQTL9
Gene Description:
high mobility group AT-hook 2
Target Gene Accession:
NM_003483.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family.
Gene References into function
- findings suggest that amplification and overexpression of HMGIC and possibly MDM2 might be important genetic events that may contribute to malignant transformation of benign pleomorphic adenoma
- Overexpression of HMG1C does not depend on chromosomal rearrangements in radiation associated pleomorphic adenomas
- Based on sequence analysis of the entire intron 3 of HMGA2, a possible new exon of HMGA2 is described, the expression of which in a transcript may have more striking effects than wild-type HMGA2 in terms of tumorigenesis.
- HMGIC alterations in smooth muscle tumors of soft tissues and other sites
- Expression of the HMGA2-LPP fusion transcript in only 1 of 61 karyotypically normal pulmonary chondroid hamartomas.
- HMGA2 promoter is coregulated by a polymorphic dinucleotide (TC)-repeat
- Fusion transcripts involving HMGA2 are not a common molecular mechanism in uterine leiomyomata with rearrangements in 12q15.
- Expression of this protein's mRNA in the peripheral blood is an independent poor prognostic indicator for survival in metastatic breast cancer.
- Results support a model in which the expression of the wild-type HMGA allele is critical for the pathogenesis of mesenchymal tumors and in which rearrangements of HMGA do not lead to a gain of function in the chimeric HMGA protein
- Histone deacetylase inhibition is associated with transcriptional repression of the Hmga2 gene.
- HMGA2 expression is required in normal adult myometrial physiology.
- Dysregulation and overexpression of HMGA2 in myeloid progenitors contribute to the pathogenesis of myelofibrosis with myeloid metaplasia.
- HMGA2 was expressed in 4 of six soft tissue chondromas, all displaying 12q-rearrangements at cytogenetic analysis.
- HMGA2 associates with the E1A-regulated transcriptional repressor p120(E4F), interfering with p120(E4F) binding to the cyclin A promoter.
- we propose that an increased expression level of the HMGA2 protein is closely associated with the malignant phenotype in the pancreatic exocrine system.
- HMGA2 overexpression is associated with aggressiveness of oral carcinoma
- Geme ay be involved in growtwh of tumors.
- Abnormalities of HMGA2 play an important and previously unsuspected role in myelodysplasia.
- HMGA2-dependent tumorigenesis is caused by a disturbed equilibrium in the co-expression of the HMGA2 splice variants leading to aberrant cell proliferation and/or malignant transformation of cells.
- Enhanced cytotoxicity by double-strand breaks in HMGA2-expressing cells is mediated, at least in part, through the signaling pathway of which the physiologic function is to maintain genome integrity.
- HMGA2, although obviously contributes to invasion, is not a specific marker for the detection of circulating tumor cells in breast cancer.
- Active expression of HMGA2 found in serous carcinoma of ovarian epithelial cells.
- Translocation in chromosome 3 and 12 involves fusion of this protein with LPP protein in pulmonary chondroid hamartoma.
- Pathogenetically significant event is fusion, truncation or transcriptional activation of HMGA2 contributes to lipoma development.
- HMGA2 gene is overexpressed in pituitary adenomas and thus plays a role in pituitary oncogenesis
- HMGA2-LPP fusion promotes chondrogenesis by upregulating cartilage-specific collagen gene expression through the N-terminal DNA binding domains.
- Endogenous HMGA2 mediates Epithelial-mesenchymal transition (EMT) by TGF-beta, whereas ectopic HMGA2 causes irreversible EMT characterized by severe E-cadherin suppression.
- expression of HMGA2 RNA is repressed in human cytomegalovirus infected cells; findings suggest that IE2 86 is involved in the inhibition of HMGA2
- The three novel transcripts, A15, B6 and D12 are located within the HMGA2 gene itself and are transcribed from the opposite strand.
- HMGA2 interacts with nucleosomes in a way that imposes a global effect on the state of ES cell chromatin, which may contribute to the establishment of both ES cell identity and the initiation of specific differentiation programs.
- These studies validate the observation that HMGA2 plays a prominent role in governing genotoxic responses.
- it is reported that chromosomal translocations previously associated with human tumors disrupt repression of Hmga2 by let-7 miRNA
- HMGA2 plays a central role in the pathogenesis of Lymphangiomyomatosis.
- We provide evidence that there is a strong and statistically significant correlation between HMGA2 and IMP2 gene expression in human liposarcomas.
- Ectopic expression of let-7 miRNA reduced HMGA2 and cell proliferation in a lung cancer cell.
- HMGA2 overexpression is associated with non-small cell lung cancer
- establish HMGA2 as a regulator of human genes linked to mesenchymal cell differentiation, adipogenesis, and human embryonic stem cell growth
- The diagnostic usefulness of HMGA2 rearrangements to differentiate between AAM and other tumors of the lower genital tract may be limited due to the their low frequency
- variant of HMGA2 is associated with adult and childhood height in the general population
- HMGA2 overexpression is associated with polycythemia vera
- HMGA2 has a role in chronic myelogenous leukemia [comment]
- up-regulation of HMGA2 expression is correlated to the undifferentiated phenotype of leukaemic cells accumulating during progression of chronic phase to blast crisis
- the different profiles of HMGA2 protein expression in post-pubertal testicular tumours could represent a valuable diagnostic tool in some cases in which the histological differential diagnosis is problematic
- HMGA2 stand-alone marker expression has a high potential to improve diagnoses of follicular neoplasms of the thyroid.
- Malignant thyroid tumors express HMGA2 up to 400-fold more than benign tumors. Sensitivity 96%, Specificity 94%.
- Expression of HMGA2 represents an early event during cancer progression.
- HMGA proteins are overexpressed in different tumors and the HMGA genes are often involved in chromosomal rearrangements [review]
- HMGA2 was amplified and rearranged in well-differentiated and dedifferentiated liposarcomas
- data support the quantitative RT-PCR analysis of HMGA2 expression, rather than immunohistochemistry, as a powerful tool for the diagnosis of thyroid neoplasias.
- Let-7-mediated repression of HMGA2 mechanism can be an important molecular event in leiomyoma growth.
- High expression of HMGA2 in gastric cancer correlates with tumor invasiveness and is an independent prognostic factor.
- HMGA2 silencing suppresses ovarian cancer cell proliferation in cases of high initial level of its expression.
- HMGA2 is an oncogene important in the pathogenesis of human lung cancer
- results identify HMGA2 and MDM2 as amplification targets in pleomorphic adenoma and carcinoma ex pleomorphic adenoma of the salivary glands
- HMGA2 cooperates with the TGF-beta/Smad pathway in regulating SNAIL1 gene expression.
- a role for HMGA2 in the progression of pancreatic ductal adenocarcinoma and suggest that it could be a useful biomarker and rational therapeutic target in more advanced disease.
- maintains neural stem cell function in young mice through repression of the Ink4a/Arf locus
- Cells of the amniotic fluid strongly overexpress HMGA2 according to their fetal origin.
- Importance of HMGA2 in reversibly maintaining mitotic activities and epithelial-mesenchymal transition suggests importance as a potential target in pancreatic cancer.
- an increased CCNB2 expression in human pituitary adenomas of different histotypes that is directly correlated with HMGA1 and HMGA2 expression.