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Validated All-in-One™ qPCR Primer for BIRC7(NM_139317.1) Search again
Product ID:
HQP019044
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
KIAP, LIVIN, ML-IAP, MLIAP, RNF50
Gene Description:
baculoviral IAP repeat containing 7
Target Gene Accession:
NM_139317.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide. [provided by RefSeq].
Gene References into function
- SMAC negatively regulates the anti-apoptotic activity of melanoma inhibitor of apoptosis (ML-IAP).
- alpha isoform of LIVIN, which is not expressed in normal bladder tissue, is expressed in a proportion of bladder carcinoma with a high risk of relapse
- livin gene is susceptible to efficient and specific silencing by the siRNA technology
- T cells in a large proportion of melanoma patients infiltrating the tumor or circulating in the peripheral blood specifically recognize ML-IAP-derived peptides
- livin gene may play a role in NSCLC development and increased expression of livin mRNA may serve as a new target for lung cancer treatment as well as survivin
- Like survivin, livin may act as a major cancer antigen in breast cancer patients
- Livin beta isoform plays the key role for the antiapoptotic protection of HeLa cells by the livin gene. the Livin isoforms can strongly differ in their functional significance for the antiapoptotic resistance of tumor cells.
- findings provide a novel function of Livin: it exhibits E3 ubiquitin ligase activity to degrade the pivotal apoptotic regulator Smac/DIABLO through the ubiquitin-proteasome pathway.
- Livin cleavage in melanoma cells occurs in an Omi-independent fashion at residue 52 within its potential caspase substrate and suggests the involvement of a non-canonical caspase.
- the BIRC7 gene might play a role in the development and aggression of non-Hodgkin's lymphoma
- Targeted inhibition of livin strongly sensitized NSCLC cells to different pro-apoptotic stimuli, such as UV-irradiation or the chemotherapeutic drug etoposide thus Livin is an important contributor to the apoptosis resistance of NSCLC cells.
- The Livin expression is a novel prognostic marker in childhood ALL and thus needs to be incorporated into the patient stratification and treatment protocols.
- Deguelin has great potential for chemosensitization of this protein and could represent a new therapeutic agent for treatment of breast cancer.
- study demonstrates that only the first N-terminal glycine residue dictates the peri-nuclear distribution of tLivin; once it is properly localized, an intact RING domain enables pro-apoptotic function
- Livin can contribute significantly to the apoptosis resistance of renal cell carcinoma cells.
- Livin expression is detected in 59 (38.8%) of 152 renal cell carcinoma specimens but not any in normal samples.
- High Livin expression is associated with osteosarcoma
- Increased livin protein expression is associated with renal cell carcinoma
- Livin beta moderately protects against NK cell killing whereas Livin alpha augments killing. Livin augments an extrinsic inhibitory signal rather than functioning as an independent inhibitory mechanism.
- Livin expression may have a role in the development of bladder cancer
- MITF is a major transcriptional regulator of ML-IAP expression in melanomas.
- Livin is implicated in testicular tumorigenesis and to be related to the histological testicular germ cell tumors subtype.
- review of the current understanding of the versatile roles of Livin in the apoptotic cascade [review]