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Validated All-in-One™ qPCR Primer for TRIM25(NM_005082.4) Search again
Product ID:
HQP018684
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EFP, RNF147, Z147, ZNF147
Gene Description:
tripartite motif containing 25
Target Gene Accession:
NM_005082.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm.
Gene References into function
- Efp controls breast cancer growth through ubiquitin-dependent proteolysis of 14-3-3sigma.
- Results indicate that estrogen-responsive finger protein is widely expressed and may play important roles in various human tissues, possibly through estrogen receptors.
- Over expression of Estrogen-responsive finger protein is associated with tumor progression of breast carcinoma
- 14-3-3 sigma expression inversely correlated with estrogen receptor alpha, progesterone receptor and estrogen-responsive finger protein, and positively correlated with myometrial invasion and lymph node metastasis in endometrial adenocarcinoma
- Efp is an IFN-responsive gene that potentially mediates IFN actions, involved in ISGylation and ubiquitination of proteins including Efp itself.
- Our results indicate that EFP functions as a cofactor for ERalpha-mediated transcription.
- role of the intact tandem CARD for TRIM25-mediated RIG-I activation; TRIM25 binds the RIG-I first CARD and subsequently ubiquitinates its second CARD
- estradiol and tamoxifen may regulate the growth of endometrial carcinoma cells by stimulating VEGF production through Efp.