|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for XPO1(NM_003400.3) Search again
Product ID:
HQP018561
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CRM-1, CRM1, emb, exp1
Gene Description:
exportin 1
Target Gene Accession:
NM_003400.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
The protein encoded by this gene mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1. [provided by RefSeq].
Gene References into function
- CRM1 is a member of the importin-beta family of proteins
- CRM1-dependent function of a cis-acting RNA export element
- The carboxy-terminal region of the human immunodeficiency virus type 1 protein Rev has multiple roles in mediating CRM1-related Rev functions.
- Down-modulation of Sam68 expression caused exclusive nuclear retention and colocalization of both Rev and CRM1.
- Reconstitution of nuclear protein export in isolated nuclear envelopes
- Rev is necessary and probably also sufficient for the accumulation of incompletely spliced HIV RNAs at the nuclear pores while CRM1 is needed for translocation across the nuclear pore complex
- CRM1-dependent nuclear export of Smurf1 is essential for the negative regulation of TGF-beta signaling by Smad7.
- Crm1 has a role in hepatitis B virus X protein-induced aberrant centriole replication and abnormal mitotic spindles
- CRM1 binds to ERK3and promotes the cytoplasmic relocalization of ERK3. Enforced localization of ERK3 in the nucleus or cytoplasm markedly attenuates the ability of the kinase to induce cell cycle arrest in fibroblasts.
- used a series of mutants, which were generated by interchanging residues of CRM1s, to examine the relationship of CRM1 functions
- CRM1 has a role in mediating a nuclear export signal to govern nuclear accumulation of glyceraldehyde-3-phosphate dehydrogenase following genotoxic stress
- CRM1 binds to Axin in the presence of RanGTP
- Activation-induced deaminase is actively excluded from the nucleus by an exportin CRM1-dependent pathway.
- CRM1-dependent mRNA export may not be mediated via the adenylate uridylate-rich element.
- Heme regulates gene expression by triggering CRM1-dependent nuclear export of BACH1.
- Data show that an isoform of Staufen2, Stau2(59), is exported from the nucleus by two distinct pathways, one of which is RNA-binding domain 3-mediated and the other of which is CRM1 (exportin 1)-dependent.
- C-terminal sequences direct cyclin D1-CRM1 binding
- CRM1-dependent nuclear export pathway is involved in the regulation of IRF-5 subcellular localization
- structural model of human CRM1 based on a combination of X-ray crystallography, homology modeling, and electron microscopy
- PHAX and CRM1 have roles in transporting U3 snoRNA to nucleoli
- Data suggest that CRM1 is able to bind cargo in the nucleolus, and upon RanGTP binding a functional export complex is produced that is exported to the cytoplasm.
- CRM1 regulates active export of Fanconi anemia complementation group A (FANCA)protein out of the nucleus
- Nuclear targeting of adenovirus type 2 requires XPO1-mediated nuclear export.
- Crm1 and Ran-GTP are essential for Ran-BP2/Ran-GAP1 recruitment to kinetochores, for definition of kinetochore fibres and for chromosome segregation at anaphase. Thus, Crm1 is a critical Ran-GTP effector for mitotic spindle assembly and function.
- These data demonstrate that Arg-214/215 are involved in CRM1-mediated STAT3 nuclear export and the regulation of STAT3 activity.
- Review proposes that Ran/Crm1 may act as a 'loading dock' to coordinate various checkpoint factors in regulating the fidelity of centrosome duplication during cell cycle progression.
- the HuR-CRM1 axis affects the nucleocytoplasmic translocation of CD83 mRNA under regular physiological conditions
- A study evaluting the species-specific barrier of HTLV-1 by establishing rat cell lines, including both adherent and CD4(+) T cells, which express human CRM1 at physiological levels, is reported.
- We found that nuclear export of EGFR may involve CRM1 exportin as we detected EGFR/CRM1 interaction and markedly increased nuclear EGFR following exposure to leptomycin B, a CRM1 inhibitor.
- role of the CRM1-dependent export pathway for the expression of CD83 and other genes under conditions of T cell activation
- the Nup214-Nup88 nucleoporin subcomplex is required for CRM1-mediated 60 S preribosomal nuclear export
- Results suggest that decreased expression of exportin-1 and exportin-t induced by 1alpha,25-dihydroxyvitamin D3 can be correlated to inhibition of the proliferation of HL-60 cells.
- The Nup214/Nup88 complex is required for efficient CRM1-mediated transport, supporting a model involving a high-affinity binding site for CRM1 at Nup214 in the terminal steps of export.
- Cellular release of TNFalpha from stimulated leukocytes is mediated by the CRM1-dependent nuclear export mechanism.
- Our report shows the functional significance of the Survivin-Crm1 interface and provides a novel link between the mitotic effector Crm1 and the CPC.
- proof of principle that it is possible to differentially modulate the IFNgamma-induced expression of the HLA-E and HLA-A genes; stimulation of HLA-A expression by IFN-gamma requires nuclear export of HLA-A mRNA by chromosome maintenance region 1 (CRM-1).
- The export receptor CRM1 is a key player in the molecular mechanism for maintaining snRNP trafficking pathways.
- the direct interaction of survivin with the nuclear export receptor Crm1 is critically involved in its intracellular localization and cancer-relevant functions [review]
- CRM1 may down-regulate Cdc7 by masking its kinase domain
- human cytomegalovirus tegument protein pp65 nuclear export requires cyclin-dependent kinase activity and the Crm1 exporter
- Inhibition of exportin-1-mediated nuclear export slowed down nucleocytoplasmic shuttling of v-Src-activated STAT3 resulting in reduced tyrosine phosphorylation, decreased induction of STAT3 target genes and increased apoptosis.
- We found no evidence that Crk II bound to Crm1 nor that the localization of GFP-Crk II was sensitive to LMB, an inhibitor of Crm1.
- Ihe subcellular localization of MSH4 is regulated by an active nuclear export pathway dependent on the exportin CRM1;CRM1 is also involved in MSH5 nuclear export.
- a functional nuclear export signal is present in the BRCA2 protein, and BRCA2 is able to exit the nucleus via the CRM1-dependent pathway
- STRADalpha facilitates nuclear export of LKB1 by serving as an adaptor between LKB1 and exportins CRM1 and exportin7.
- Immunohistochemistry revealed increased nuclear (52.7%) and cytoplasmic (56.8%) expression of CRM1 in 74 carcinomas compared with the expression revealed in borderline tumors and benign lesions.
- CRM1-mediated nuclear export may be required for the proper execution of ubiquitin-proteasome-dependent degradation of TRIP-Br2
- Phosphorylation of ChREBP was essential for its interaction with CRM1 for export to the cytosol, whereas nuclear import of ChREBP requires dephosphorylated ChREBP to interact with importin alpha.
- NucNeoR gained access to this autophagy-dependent MHC class II presentation pathway by a CRM1-independent route.
- TRalpha may follow a cooperative export pathway in which both calreticulin and CRM1 play a role in facilitating efficient translocation of TRalpha from the nucleus to cytoplasm
- Atypical CRM1-dependent nuclear export signal mediates regulation of hypoxia-inducible factor-1alpha by MAPK.
- herpes simplex virus type 1 UL47 joins a growing list of virus-encoded RNA-binding proteins that use CRM1 to exit the nucleus.
- Results show that CPEB1 is continuously shuttling between nucleus and cytoplasm, and its export is controlled by two redundant motifs dependent on the nuclear export receptor Crm1.
- of CRM1 was an independent prognostic parameter for longer overall survival in human osteosarcoma
- Crm1, Kpnbeta1 and Kpnalpha2 are overexpressed in cervical cancer and inhibiting the expression of Crm1 and Kpnbeta1, not Kpnalpha2, induces cancer cell death