|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for XBP1(NM_001079539.1) Search again
Product ID:
HQP018550
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
TREB-5, TREB5, XBP-1, XBP2
Gene Description:
X-box binding protein 1
Target Gene Accession:
NM_001079539.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1).
Gene References into function
- XBP1 is a key regulator of mammalian ER stress response
- down-regulation of expression correlate with prostate adenocarcinoma progression
- XBP-1 and activation of cAMP response elements expression are upregulated in acquired antiestrogen resistance in breast cancer cells
- XBP1 and ATF6 jointly regulate mammalian ER stress response
- The endoplasmic reticulum stress pathway mediated by ATF6 and by IRE1-XBP1 systems seems essential for the transformation-associated expression of the grp78 gene in hepatocarcinogenesis
- Impaired feedback regulaton of this protein is a genetic risk factor for bipolar disorder.
- XBP-1 is implicated in a gene network that regulates estrogen receptor signaling in breast cancer
- increased expression of the hXBP-1 gene may play some role in human breast carcinogenesis through impairment of cell differentiation regulation
- By subjecting dermal fibroblasts to multiple stresses, strong correlations were found between ATF6 activation and XBP1 splicing, and between GRP78/BiP mRNA and EDEM mRNA accumulation.
- Data suggest that -197C/G in XBP1 is also a genetic risk factor for schizophrenia, and presents a sex-dependent genetic effect for the disorder.
- XBP-1 has a role in ERalpha transcriptional activity through regulation of large-scale chromatin unfolding
- ATF6 and XBP1 have roles in activation of endoplasmic reticulum stress-responsive cis-acting elements
- results reveal a target sequence for the IRE1-XBP1 pathway under ER stress conditions
- Transcriptional regulator XBP-1 governing B-cell terminal cellular differentiation provides multiple myeloma-and other tumor- and nonmalignant-associated cytotoxic T-lymphocyte epitopes.
- The present results suggested that the XBP1 gene polymorphism was associated with the NEO-FFI score of neuroticism in female subjects.
- The functional XBP1 gene polymorphism is not of major importance to schizophrenia in the European populations investigated.
- The swift adapting to physiological changes in the endoplasmic reticulum was studied which required the interplay between XBP1(unspliced) and XBP1 (spliced).
- XBP1 pre-mRNA encodes a negative feed-back regulator of ER stress response
- XBP1 splicing can occur in the cytoplasm, and that cleavage and ligation of XBP1 mRNA during splicing may occur as a coupled reaction.
- Our results clearly establish HBx as an inducer of UPR and the activator of the ATF6 and IRE1-XBP1 pathways of UPR.
- Overexpression of XBP-1 is associated with multiple myeloma pathogenesis
- Misoxidised MHC class I heavy chains activate XBP1 processing in a similar manner to tunicamycin.
- Overexpression of spliced XBP1 prevents cell cycle arrest and antiestrogen-induced cell death through the mitochondrial apoptotic pathway.
- XBP1 and the unfolded protein response modulate responsiveness to estrogens and antiestrogens in breast cancer.
- We report that the plasma cell differentiation factor, XBP-1s, activates the expression of the master regulator of EBV lytic activation, BZLF1.
- When islets were cultured for 24 h at 11.1 mmol/l glucose, there was induction of BiP and XBP-1 in type 2 diabetes islets but not in non-diabetic islets.
- XBP-1s binds to and activates the KSHV immediate-early gene ORF50.
- XBP1 polymorphism (-116C/G) is not involved in susceptibility to suicide.
- Nonalcoholic steatohepatitis is specifically associated with (1) failure to generate sXBP-1 protein and (2) activation of JNK.
- that transcription factors Xbp-1, Blimp-1, and PAX-5-encoded BSAP play important roles in the regulation of plasmacytic differentiation and exert their effects on differentiation induced by low 2ME2 concentrations
- Findings, that XBP-1 isoforms are differently associated with outcome of endocrine therapy for patients, can be explained by higher levels of dominant-negative XBP-1U favouring apoptosis of tumor cells and higher levels of XBP-1S increasing tumor surviva
- Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum inhibit secretion of misfolded protein conformers and enhancing their disposal
- By chromatin immunoprecipitation and assays using an inducible Spi-B construct BLIMP1 and XBP-1 were identified as direct target genes of Spi-B mediated repression.
- Compared with COPD and donor lungs, protein levels of ER stress mediators, such as ATF-6 and ATF-4 and the apoptosis-inductor CHOP as well as XBP-1, were significantly elevated in lung homogenates and AECIIs of IPF lungs
- An association of XBP1 variants with both forms of human IBD (Crohn's disease and ulcerative colitis) was identified and replicated (rs35873774; p value 1.6 x 10(-5)) with novel, private hypomorphic variants identified as susceptibility factors.
- Transcriptional induction of the human asparagine synthetase gene during the unfolded protein response does not require the XBP1.