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Validated All-in-One™ qPCR Primer for WEE1(NM_003390.3) Search again
Product ID:
HQP018519
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
WEE1A, WEE1hu
Gene Description:
WEE1 G2 checkpoint kinase
Target Gene Accession:
NM_003390.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq].
Gene References into function
- Wee-1 may serve as a key regulator of both HIV type 1 Vpr- and gamma irradiation-mediated apoptosis
- increases of cyclin D1, cyclin-dependent kinase 4, cyclin E, cyclin A, and Wee1 play an important role in the development of hepatocellular carcinoma from cirrhosis
- Proteasome-dependent degradation of this protein is inhibited in G2-arrested Hep3B cells by TGF beta 1.
- WEE1 is directly transactivated by and increased in association with c-Fos/AP-1.
- Loss of Wee1 expression may have a potential role in promoting tumor progression and may be a significant prognostic indicator in NSCLC.
- beta-TrCP-dependent degradation of Wee1A is important for the normal onset of M-phase in vivo.
- the induction of Cdc2 phosphorylation due to the increase of Wee1 and Myt1 as well as the reduction of Cdc2 and cyclin B1 are involved in 1,25[OH]2VD3-induced G2/M arrest of keratinocytes.
- Inhibiting Hsl7 delayed mitosis but overexpression overrode the replication checkpoint, accelerating Wee1 destruction. Checkpoint activation disrupted Hsl7-Wee1 binding which was restored by polo-like kinase. Hsl7 is a replication checkpoint component
- Level of WEE1 is regulated by KLF2 and enhanced KLF2 expression sensitizes cells to DNA damage-induced apoptosis.
- Akt protein phosphorylation and inactivation of WEE1 promote cell cycle progression at G2/M transition.
- our studies indicate that the 2-ME(2)-induced upregulation of wee1 and subsequent cdc2 phosphorylation are mediated through mitogen-activated protein kinase (MAPK)-ERK-JNK signaling pathways.
- Gamma-irradiated human primary prostate epithelial cells were unable to enforce cell cycle checkpoint arrest and had sustained Cdk2-associated kinase activity because of a lack of inhibitory Cdk phosphorylation by Wee1A tyrosine kinase.
- WEE1 can be introduced to rescue cells from the damage caused by certain antineoplastic agents.
- Significant percentage of the total endogenous Crk II partitions in the nucleus in mammalian cells, where it forms distinct complexes with DOCK180, Wee1, and Abl.
- HIV-1 Vpr binds to the N lobe of the Wee1 kinase domain and enhances kinase activity for CDC2.
- These findings support the view that CK2beta regulates various intracellular processes by modulating the activity of protein kinases that are distinct from CK2.
- HSP90 inhibition abrogates the topoisomerase I poison-induced G2/M checkpoint in p53-null tumor cells by depleting CHK1 and WEE1.