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Validated All-in-One™ qPCR Primer for EZR(NM_003379.4) Search again
Product ID:
HQP018488
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CVIL, CVL, HEL-S-105, VIL2
Gene Description:
ezrin
Target Gene Accession:
NM_003379.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq].
Gene References into function
- A2BR binds to Ezrin upon agonist stimulation.
- evaluated the role of Lck tyrosine kinase, an early effector of T cell activation, in regulation of the membrane-cytoskeleton linker protein ezrin; results identify ezrin as the first cytoskeletal substrate for Lck
- Although ezrin is excluded from the inhibitory, i.e., noncytolytic, NK cell immune synapse (IS), it is homogeneously distributed across the IS of activating conjugates.
- Review.CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.
- amino-terminal domains of the ezrin, radixin, and moesin (ERM) proteins bind advanced glycation end products and have a role in diabetes
- The upregulated ezrin expression is one of the important factors that are possibly associated with the invasive phenotype formation in malignantly transformed esophageal epithelial cells (ezrin)
- Ezrin N-terminal domain binds to the syndecan-2 cytoplasmic domain with an estimated K(D) of 0.71 microM and without the requirement of other proteins
- a major role of ezrin is to connect CD95 to actin, thus allowing the CD95 polarization on the cells and the occurrence of the following multiple cascades of the CD95 pathway
- Data show that ezrin and the homeodomain-containing transcription factor Six-1 (sine oculis-related homeobox-1 homolog) had essential roles in determining the metastatic fate of rhabdomyosarcoma cells.
- results report a significant association between high ezrin expression and poor outcome in pediatric osteosarcoma patients
- pka phosphorylation of merlin serine 518 promotes heterodimerization between merlin and ezrin, an event suggested to convert merlin from a growth-suppressive to a growth-permissive state
- ezrin/radixin/moesin proteins are recruited by NHE1 Na+/H+ exchanger and have roles in regulating Akt-dependent cell survival
- All three ERM family members can localise to the nucleus; a specific nuclear localisation sequence, which is conserved and functional in all ERM family members, is identified, implying specific regulated nuclear import.
- results suggest that ezrin-radixin-moesin proteins are required for microvillar positioning of L-selectin and that this is important both for leukocyte tethering and L-selectin shedding
- Ezrin, radixin and moesin are ADP-ribosylated by Pseudomonas aeruginosa ExoS
- Akt2-dependent ezrin phosphorylation leads to NHE3 translocation and activation
- Src phosphorylates ezrin at tyrosine 477
- Src-dependent ezrin phosphorylation has a role in adhesion-mediated events in epithelial cells
- Data show that the integrity of ezrin is essential for parietal cell activation and provide evidence that ezrin interacts with PALS1, an evolutionarily conserved PDZ and SH3 domain-containing protein.
- Ezrin accumulates at Listeria protrusions, which plays a role in the Listeria cell-to-cell spread during infection.
- Results strongly support the conformational activation model of ezrin, elucidate the basis for dimer formation, and reveal that a mutant generally considered to be fully activated is not.
- Ezrin is a novel substrate of GRK2
- ezrin binding activates the second PDZ domain of NHERF to interact with the cytoplasmic tails of CFTR (C-CFTR), so as to form a specific 2:1:1 (C-CFTR)(2).NHERF.ezrin ternary complex
- High expressions of ezrin is associated with development of distant metastasis of soft tissue sarcomas
- Ezrin was expressed in the majority of prostate cancers and correlated with adverse prognostic factors
- Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling.
- Protein Kinase A-mediated phosphorylation of ezrin is essential and sufficient for the apical localization of WW domain containing oxidoreductase (WWOX) protein as disruption of ezrin-WWOX interaction eliminated the apical localization of WWOX
- a change in ezrin localization from the apical membrane to either the complete membrane or to the cytoplasm may have a role in dedifferentiation in invasive breast tumors
- Elevated ezrin expression is a new independent prognostic marker in FIGO stage I endometrioid carcinoma, and thus provides further evidence for an important role of ezrin in tumor progression.
- data imply that ezrin is necessary for tumor cell invasion, and the better prognosis of ovarian carcinomas lacking ezrin is probably related to their impaired invasion
- the release of ezrin from lipid rafts acts as a critical trigger that regulates lipid raft dynamics during B cell antigen receptor signaling.
- Ezrin appears to play a role in the progression of tumors, such as the metastasis of osteosarcoma.
- Hormonal regulation of ezrin phosphorylation is required for androgen-induced cell invasion.
- Expression of ExoS in HeLa cells led to a loss of phosphorylation of Ezrin/radixin/moesin proteins that was dependent upon the expression of ADP-ribosyltransferase activity.
- the action of ezrin in Ewing's sarcoma is dependent on the AKT/mTOR signal transduction cascade, but not MAP Kinase.
- increased CD44, ezrin, radixin, and moesin phosphorylation represents a key molecular abnormality that guides T cell adhesion and migration in SLE patients.
- identification of PTHrP and ezrin as important regulators of lung cancer bone metastasis offers new mechanistic insights into the metastasis of lung cancer and provides potential targets for the prevention and treatment of lung cancer metastasis
- data support a role for ezrin in the biology of human cancers and the need for additional studies in breast cancer and sarcoma patients that may validate ezrin as a marker of cancer progression and as a potential target for cancer therapy
- ezrin was differentially expressed in sclerotic hippocampi compared to non-sclerotic ones.
- Ezrin is de-expressed in endometriosis and endometrioid carcinoma compared with normal uterine epithelium.
- These data suggest a mechanism whereby precise spatial guanine nucleotide exchange of Cdc42 by Dbl is dependent on functional ERM proteins and is important for directional cell migration.
- podocalyxin leads to increased in vitro migration and invasion, increased MMP expression, and increased activation of MAPK and PI3K activity in MCF7 and PC3 cells through its ability to form a complex with ezrin
- The low expression of ezrin implicates the loss of adhesion in diffuse carcinomas. overexpression of ezrin in carcinomas with H pylori infection may be a pathway in which H pylori may cause/initiate gastric carcinoma.
- Signals from the extracellular association CD44-ezrin-actin are an important modulator of Fas-mediated apoptosis.
- Ezrin and alpha-smooth muscle actin are predictive immunohistochemical prognostic markers for patients with an osteosarcoma
- Radixin and ezrin play similar roles in the apical membrane localization of ABCC2 (MRP2) and their expression level and subcellular distribution are important factors in the regulation of ABCC2 (MRP2) at the post-transcriptional level.
- ezrin allows the local activation of RhoG at the apical pole of epithelial cells by recruiting upstream and downstream regulators of RhoG and that both PLEKHG6 and ezrin are required for efficient macropinocytosis
- pattern of ezrin staining can identify patients with different risks of relapse of non-metastatic osteosarcoma of the extremity
- Ezrin via ERM-binding phosphoprotein 50 (EBP50) is the essential link between cytoskeletal F-actin and a lipid raft-associated multiprotein complex that negatively regulates T cell immune responses.
- Ezrin/Fes interaction at cell-cell contacts plays an essential role in hepatocyte growth factor-induced cell scattering.
- The identification of Ezrin as a specific and direct cellular substrate of PRL-3, is reported.
- These data indicate that ezrin overexpression positively correlated with metastatic potentials of human breast cancer cells, especially lymphatic system metastasis.
- ezrin peptide (aa 277-299) dose-dependently reversed the inhibitory effect of glycated albumin on ezrin (1-324) phosphorylation in vitro, suggesting that binding of advanced glycation endproducts to ezrin changes the conformation of the latter
- Apically localized phosphorylation by PKCiota is essential for the activation and normal distribution of ezrin at the early stages of intestinal epithelial cell differentiation.
- the ezrin, radixin, and moesin proteins function as positive regulators of infection by X4-tropic HIV-1
- RNA interference to ezrin increases the virus susceptibility of human TE cells
- Hepatocellular carcinoma with ezrin expression might be at least partly derived from hepatic progenitor cells.
- supports a role of ezrin in progression in human prostate cancers
- Ezrin's localization and association with cell specializations indicate that in the vagina ezrin likely modulates vaginal cell-cell interactions
- a specific domain in Fas, topologically and functionally distinct from the death domain, which regulates neuritogenesis via recruitment of ezrin and activation of Rac1
- Ezrin expression in hepatic malignant fibrous histiocytoma can provide additional prognostic information and may be a potential target for new adjuvant therapies.
- Ezrin is a potential marker for endometrial cell invasion and is associated with the pathogenesis of endometriosis.
- Ezrin may play a role in colorectal cancer progression and that ezrin expression might provide clinically valuable information in predicting the biological behavior of colorectal cancer.
- analysis of the Ca2+ -regulated ezrin-S100P interaction and its role in tumor cell migration
- These findings demonstrated a new role and regulatory mechanism of miR-183 in controlling cancer metastasis; Ezrin is a bona fide target of miR-183.
- Phosphorylation of ezrin/moesin by Rho-associated coiled coil-containing protein kinase (ROCK) is involved in the early steps of apoptotic signaling following Fas receptor triggering and regulates apoptosis induction in Jurkat cells.
- These observations highlight the importance of ezrin/F-actin function in the development of dynamic membrane/actin structures critical for cell shape and motility, as well as signal transduction.
- No aspects of T cell receptor signaling uniquely require transgenic ezrin or moesin; instead, T cell activation appears to depend on net ezrin, radixin, and moesin (ERM) protein expression, while ezrin and moesin function together.
- radixin, moesin and c14orf166, but not ezrin, had significantly higher expression levels in lymph node metastasis (LNM) pancreatic cancers than in non-LNM controls.