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Validated All-in-One™ qPCR Primer for TIMP2(NM_003255.4) Search again
Product ID:
HQP018093
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CSC-21K, DDC8
Gene Description:
TIMP metallopeptidase inhibitor 2
Target Gene Accession:
NM_003255.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq].
Gene References into function
- The growth of the primary melanoma cell lines was stimulated by TIMP-1 and inhibited by TIMP-2. In contrast, the growth of the visceral metastatic melanoma cell line was stimulated by TIMP-2.
- TIMP-2 may be involved in the pathogenesis of gestational trophoblastic disease
- role in activating ras proteins
- endometrium from women with endometriosis expressed higher levels of MMP-2 and MT1-MMP and lower levels of TIMP-2 than did endometrium from normal women
- investigation of structural and functional differences between TIMP-4 and TIMP-2 by mutagenesis into C-terminal tails
- This protein is present in normal and azoospermic human semen.
- correlation between expression of MMP-2, TIMP-2 protein and the ratio of MMP-2/TIMP-2 and clinical-pathological parameters of patients with gallbladder carcinoma
- TIMP2 expression is regulated by the ERK/MAPK pathway
- Changes in plasma MMP-2 and TIMP-2 concentrations during cardiopulmonary bypass may play an important role in LV remodeling after cardiac surgery.
- Progress curve analysis of MMP inhibition by TIMP-2 shows that it has lower reactivity with MMPs at less acidic conditions than TIMP-4.
- The over-expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 may play a key role in invasion and lymph-node metastasis of in squamous carcinoma of the cervix.
- TIMP-2 expression in breast cancer is related to survival.
- TIMP-2 was significantly increased in CSF of Alzheimer's disease and Huntington's disease patients.
- TIMP-2 mediated inhibition of angiogenesis is an MMP-independent mechanism.
- TIMP-2 possesses two distinct types of anti-angiogenic activities which can be uncoupled from each other
- TIMP-2 expression is markedly increased in clear cell carcinoma of the ovary, suggesting a role of TIMP-2 in its unique characteristics among ovarian cancers
- palpable tumours demonstrated significantly more MMP-2 and significantly less MMP-9 expression than nonpalpable tumours
- A bivariate analysis revealed a modest but significant genetic correlation between TIMP-1 and TIMP-2.
- A gradual, time-dependent decline in TIMP-2 mRNA expression was observed in the presence of the synthetic androgen analogue R1881.
- Our analysis of the entire coding region of TIMP-1, -2, and -3, which are the main inhibitors of metalloproteinase activity in the extracellular matrix, failed to show an association between genetic polymorphisms and an intracranial aneurysm
- TIMP-2 stromal cell expression only was associated with adverse prognosis of urothelial bladder cancer patients.
- effect of titanium, zirconia and alumina ceramics on activity and secretion in human osteoblasts
- Tissue from lateral and medial rectus muscles had different levels of expression of TIMP-1 and 2, MMP-2, and BMP-4, which may underlie different characteristics of these extraocular muscles and may influence wound healing after strabismus surgery.
- TIMP-2 is hypermethylated in human cervical cancer
- MMP-26 was colocalized with MMP-9, TIMP-2, and TIMP-4 in breast cancer cells.
- TIMP-2 is shown to correlate with systemic symptoms in Hodgkin lymphoma
- MMP and TIMP may be involved in the feto-neonatal development and may contribute to the pathogenesis of bronchopulmonary dysplasia and/or intraventricular hemorrhage in critically ill preterm neonates
- MMP-2, TIMP-1 and TIMP-2 are downregulated in human orthotopic liver transplantation
- hereditary neuralgic amyotrophy is not caused by point mutations of tissue inhibitor of metalloproteinase 2
- TIMP-2 correlated negatively with fractional shortening & positively with LV dimension. Changes in the release & activity of TIMP-2 may be associated with the mechanisms responsible for cardiac remodeling in patients with hypertrophic cardiomyopathy.
- TIMP2 promotes growth of A549 lung cells, accompanied by increase in NF-kappaB activity and downregulation of IkappaBalpha and beta proteins and later increases in Bcl-3, IkappaB, and cyclin D1 proteins.
- Dermal wound healing in red Duroc pigs show unique mRNA expression of HSP47,BMP-1,TIMP1-3 and hypercontracted,hyperpigmented scars.
- ERK 1/2- and p38 MAPK-modulated TIMP-2 expression regulates MT1-MMP activity and control TGF-beta1-induced pericellular collagenolysis
- marked difference in MMPs and their inhibitors in the in vitro fertilized women and normally ovulating women
- Plasma levels are increased in type 2 diabetic patients
- analysis of the molecular basis of the inactivity of tissue inhibitor of metalloproteinase-2 against tumor necrosis factor-alpha-converting enzyme
- data suggest that the MMP-2/TIMP2 system and an activated extrinsic coagulation pathway could cooperate in conditions of elevated SOX and could influence arterial remodeling resulting in the presence of CVD in haemodialysis patients
- clearance of pro-matrix metalloproteinase (MMP)-2.tissue inhibitor of MMPs (TIMP-2) complex involves binding to the cell membrane and subsequent low density lipoprotein receptor-related protein (LRP)-dependent internalization and degradation
- The expression of MMP-9, TIMP-1 and TIMP-2 was lower in the benign and low malignancy ovarian tumors compared with the malignant ones.
- MMP-2 plays an essential role in tumor invasion and metastasis, while TIMP-2 is shown to strongly inhibit cancer invasion and metastasis.
- This study found an up-regulation of TIMP-1, TIMP-2 and MMP-2 RNA in Duchenne Muscular Dystrophy muscle.
- Expression in colorectal carcinomas relating to size and invasiveness of the tumor.
- Down-regulation of TIMP-2 is associated with nonsmall cell lung carcinoma
- altered MMP/TIMP ratios in maternal blood during gestational hypertension
- evidence that cell density influences the invasive potential of tumor cells via regulation of MMPs and TIMP-1 and TIMP-2 by AP-1, NF kappa B and CRE transcription factors
- promoter hypermethylation of TIMP-2 is a novel epigenetic event in the pathogenesis of lymphoid malignancies
- A decreased activity of TIMP-2 plays a role in inguinal hernia development.
- a magnetic resonance imaging (MRI) measure of disease activity in multiple sclerosis patients during treatment with rIFNbeta-1a.
- Cardiac expression of TIMP-1 and TIMP-2 is significantly increased in chronic pressure-overloaded human hearts compared with controls and is related to the degree of interstitial fibrosis
- TIMP-2 may play an important role in the invasion and metastasis of oral squamous cell carcinoma.
- Pro-MMP-2 and TIMP-2 levels negatively correlated in herniated discs samples
- With tumor progression and development of heterogeneity, the abnormal expressions of MMP-9, TIMP-2, and E-cadherin or DNA aneuploid rate or high SPF gradually increases, suggesting that they play a crucial role in gastric carcinoma progression
- Shp-1 mediates the antiproliferative activity of tissue inhibitor of metalloproteinase-2
- Results of this study provide evidence that TIMP-2 immunoreactive protein is prognostic in estimation of the aggressive clinical course of head and neck squamous cell carcinoma.
- The colocalization of EMMPRIN, MT1-MMP and TIMP-2 in human cervical carcinomas seems to be involved in a specific distribution pattern of tumor cell bound MMP-2, which is related with local proteolytic activity.
- TIMP-2 regulates the activity of MMPs in the extracellular matrix. The high frequency of GG genotype in the TIMP-2 gene promoter showed this site was unsuitable for studies of DNA polymorphism-disease associations in the studied population.
- TIMP-2-mediated inhibition of Src kinase activity leads to the signaling switch from Rac1 to Rap1, thereby leading to enhanced RECK expression.
- TIMP-2 reactivity in capillaries and fibroblasts was decreased in congenital diaphragmatic hernia lungs.
- TIMP-1 and TIMP-2 are major serum factors that stimulate the induction of TIMP-1 mRNA in quiescent human gingival fibroblasts (Gin-1 cells) at mid-G1 (6-9 h after serum stimulation) of the cell cycle, but not that of TIMP-2.
- TIMP-2 mediated alteration in cell morphology requires Rap1, TIMP-2 may recruit Rap1 to sites of actin cytoskeleton remodeling necessary for cell spreading, and enhanced cell adhesion by TIMP-2 expression may hinder cell migration
- Presence of a G/C heterozygous genotype at position -418 in TIMP2 promoter could be a genetic predisposing factor for familial moyamoya disease.
- an imbalance between the MMP-2 and TIMP-2, caused primarily by an increase in TIMP-2 activity, contributes to the pathogenesis of diabetic nephropathy.
- Increase in serum levels eight days after IVIG treatment in neuromuscular disease.
- When all three proteases are studied in combination, a tendency was found for tumors with high MMP-2, high MMP-14 and low TIMP-2 expression to predict a poor prognosis but the results did not reach significance
- TIMP-1 may serve as a useful laboratory marker to predict the clinical outcome of patients presenting with severe sepsis.
- TIMP-2 has a role in the pericyte-induced stabilization of newly formed vascular networks that are predisposed to undergo regression and reveal specific molecular targets of the inhibitors regulating these events.
- TIMP-2 is abundant in the ovarian perifollicular stroma. Its median level increased in the early ovulatory phase.
- Expression of TIMP-2 enhances the antitumor efficacy of a replicating adenovirus in vivo, by reducing both tumor growth and angiogenesis.
- TIMP2 and TIMP3 play functional role in LPA-induced invasion as negative regulators.
- Results describe the opposite effects of high glucose on matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in human endothelial cells.
- TIMP-1 and TIMP-2 have a differential relationship with haemostatic proteins and roles in hemostasis and thrombogenesis
- The aim of the present study was to investigate the effect of P. gingivalis on the expression and production of MMP-2, MMP-9, TIMP-1, and TIMP-2 by oral fibroblasts and epithelial cells.
- downregulation of the TIMP-2 gene is associated with promoter methylation and that this may play an important role in prostate cancer progression during the invasive and metastatic stages of the disease
- TIMP-2 polymorphism is strongly associated with an increased risk of OSCC in Europeans carrying the low C allele expression.
- Positive correlation was found between hepatosplenomegaly and the MMP-2/TIMP-2 ratios (p=0.005 and 0.009) and between CNS involvement and the MMP-2/TIMP-2 ratio
- The results indicate that TIMP-2 and MMP-2:TIMP-2 complex associate with favorable clinical course and could be used as a novel prognostic indicators in bladder cancer.
- the -418G to C gene polymorphism is associated with generalized aggressive periodontitis in Chinese subjects
- The expression of TIMP2 is significantly decreased in floppy valves compared to normal valves.
- Results of this study suggest that Systemic sclerosis patients with anticentromere antibodies positivity, after a primary fibrogenetic noxa, react with a more abundant release of MMP/TIMP, whereas patients with anti-Scl70 antibody show a normal response.
- MMP/TIMP balance seems to play an essential role in transferring mechanical signals into mesenchymal stem cells function.
- Expression of TIMP2 was evaluated in hepatocellular carcinoma and surrounding non-tumor tissue.
- incidental clear cell renal cell carcinomas strongly expressing MMP-9 and TIMP-2 have a higher nulclear grade(worse prognosis).
- Results determine the level of MMP-2, its natural inhibitor TIMP-2, their ratio and HER-2/neu as diagnostic and prognostic factors in breast cancer.
- MMP-9, but not TIMP-1 nor MMP-2 expression is increased in plaques causing acute coronary syndrome.
- Increased Timp-2 is assopciated with squamous cell laryngeal carcinoma
- elderly Chinese with TIMP2 C-allele have higher magnitude of QT dispersion and QTc dispersion prolongation
- MMP-2, MT1-MMP and TIMP-2 mRNA expression levels were correlated with clinical stages and histological subtypes of thymic epithelial tumours. The activation of pro-MMP-2 might be mediated by MT1-MMP and TIMP-2 up-regulation.
- TIMP-2 G-418C polymorphism increases risk of gastric cancer in a Chinese population, especially in younger subjects and smokers.
- gravitational unloading led to induction of TIMP2, an inhibitor of angiogenesis, in microvascular endothelial cells
- Findings illustrate a novel role for MT1-MMP-TIMP-2 interaction, which controls cell functions by a mechanism independent of extracellular matrix degradation.
- No significant difference was seen in gene expression level of MMPs, TIMPs and ratio of MMPs/TIMPs in ascending aorta and AV between patients with BAV and TAV.
- MMP-2, MMP-9, TIMP-1 and TIMP-2 play an important role in the pathogenesis of non-melanoma skin cancer. The immunoexpression of these proteins may be useful indicators of cutaneous cancer invasion and progression.
- Report association of polymorphisms of the MMP-2 and TIMP-2 genes with the risk of endometriosis in North Chinese women.
- MMP-2, TIMP-1 and TIMP-2 levels are significantly higher and MMP-9 lower in children with chronic hepatitis B
- There was correlation between TIMP-2 expression and tumor size and grading observed. We didn't find any correlation between TIMP-2 and nodal metastases, recurrence and survival.
- Crucial participants in tumour invasion and metastases are matrix metalloproteinases, tissue inhibitor of metalloproteinase inhibitors and cellular adhesion molecules. They play roles in tumour invasion and metastasis in non-small-cell lung carcinomas.
- There was no association with TIMP-2 polymorphisms and risk of adenomyosis in North Chinese women.
- TIMP-1, -2, and -3 are significantly reduced by chorionic gonadotropin in endometrial stromal cells.
- no significant association between TIMP-2 polymorphisms and Osteoarthritis was observed
- This study suggests that MMP14 is involved mostly in prostate cancer implantation and that MMP2 and TIMP2 expression by reactive stromal cells might be used as predictors of diease-free survival in T3N0-2M0 Pca.
- The TIMP-2 G-418C SNP may be associated with the risk of different histological subtypes of epithelial ovarian cancer.
- Identification of two previously unreported SNPs in FGFR1 and FABP3 associated with BMD and a third SNP in TIMP2 related to risk for non-vertebral osteoporotic fractures.
- TIMP-2 overproduction is associated with bone marrow stromal cells in multiple myeloma
- Upregulation of TIMP-2 production is one mechanism by which prodrug JS-K mediates its anti-invasive effects
- Comparison of TIMP2 levels in preeclampsia and gestational hypertension with those found in normotensive pregnancies.
- hematopoiesis was not adversely affected by TIMP-1 or TIMP-2
- Aggressive forms of gastric cancer are associated with low TIMP-2 expression.
- TIMP-2 -418G/C polymorphisms were not associated with the risk of developing endometriosis or adenomyosis
- This case-control study investigates the possible impact of genetic variants of the TIMP-2 gene in the aetiology of AAA and to reproduce a recently described significant difference in allele frequency of the SNP 303G>A in a German population.
- Serous and mucinous ovarian tumors express different profiles of TIMP-2.
- The results revealed that mononuclear cells were highly immunoreactive for TIMP-1, TIMP-2 and MMP-9 during viral meningitis and that the expression of TIMPs in polymorphonuclear cells was even higher during bacterial meningitis.