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Validated All-in-One™ qPCR Primer for THY1(NM_006288.4) Search again
Product ID:
HQP018083
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD90, CDw90
Gene Description:
Thy-1 cell surface antigen
Target Gene Accession:
NM_006288.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Review article on CD90 structure and function.
- Thy-1/Thy-1 ligand interaction may play a role in adhesion of melanoma cells to endothelial cells
- THY1 is a putative tumor suppressor gene for ovarian cancer; and THBS1, SPARC, and FN1 are genes associated with the regulation of in vivo tumor growth rate
- Fibroblast Thy-1 display pre-determines lineage to a contractile or lipid-like phenotype in the human myometrium and orbit.
- The interaction between Thy-1 and Mac-1 is involved in the adhesion of leukocytes to activated endothelial cells as well as in subsequent transendothelial migration of leukocytes into the perivascular tissue.
- THY1 can be designated as a putative tumor suppressor gene for human ovarian cancer.
- IE or E gene products of human cytomegalovirus induce a so far unidentified soluble factor that mediates Thy-1 downregulation.
- results indicate that alphaX-beta2 specifically interacts with Thy-1
- new pair of adhesion molecules, Thy-1 and alphavbeta3, identified that mediate the interaction of melanoma cells and activated endothelial cells
- THY1 is a good candidate tumour suppressor gene in nasopharyngeal carcinoma, which is significantly associated with lymph node metastases.
- Role of endothelial cell receptor Thy1 in cell adhesion was shown.
- Thy 1 mRNA levels do not reflect the number of RGCs present and extend this to include a parallel decrease in Thy1 protein levels.
- Expression of Thy1 decreased in differentiated ADSC and BMSC. Expression of albumin, CYP2E1, and CYP3A4 increased in differentiated BMSC and ADSC. Hepatic gene activation may involve increased C/EBPbeta and HNF4alpha.
- Thy-1 is not a marker of oval cells but is present on a subpopulation of myofibroblasts/stellate cells.
- Thy1-negative NBL patients have a significantly impaired overall survival compared with Thy1-positive NBL patients. Thus, Thy1 seemed to be a marker with a specific prognostic value in NBL patients.
- All the tumor specimens and 91.6% of blood samples from liver cancer patients bore the CD45(-)CD90+ population, which could generate tumor nodules in immunodeficient mice
- Results support the general concept that the function of 'adhesion molecules' in particular of Thy-1, may not only be to provide mechanical support but also regulate neutrophil functions such as extravasation and recruitment of additional neutrophils.
- Epigenetic regulation of Thy-1 is a novel and potentially reversible mechanism in fibrosis that may offer the possibility of new therapeutic options
- increased Thy-1 expression in Graves ophthalmopathy orbital tissues and cultures is likely a consequence of the orbital disease process, reflecting both the presence of increased numbers of Thy-1-positive cells and higher expression on those cells.