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Validated All-in-One™ qPCR Primer for TERT(NM_198253.2) Search again
Product ID:
HQP018017
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT
Gene Description:
telomerase reverse transcriptase
Target Gene Accession:
NM_198253.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG.
Gene References into function
- telomerase hTERT mRNA and telomerase activity in endometrioid adenocarcinoma and in normal endometrium.
- the oligomerization of hTERT is an important step for telomerase activity.
- allosteric inhibitors of telomerase: oligonucleotide N3'-->P5' phosphoramidates
- telomerase activity in needle biopsy samples is a useful diagnostic marker to distinguish uterine sarcoma from leiomyoma
- Telomerase activity in microdissected human breast cancer tissues: association with p53, p21 and outcome
- complete genomic sequence and analysis of tandem repeat polymorphisms
- histone deacetylase transactivates the hTERT gene in normal human telomerase-negative cells, and upregulates hTERT expression in telomerase-positive tumor cells
- Losses of INK4a/INK4b gene products play a big role in meningioma formation & malignant progression. Inactivation of p16/p15 and pl4ARF pendent pathways possibly along with telomerase activation might be critical for meningioma immortalization.
- telomerase activity is regulated, in part, by alternative splicing of hTERT
- Introduction of hTERT into activated human hepatic stellate cells (HSCs) immortalizes them and maintains their activated phenotype.
- High telomerase activity coincides with genome stability and DNA ploidy in renal cell carcinoma
- inhibition of promoter activity in breast cancer by a complex of BRCA1, Nmi and c-Myc
- increased expression is related to hypermethylation in colorectal cancer tissues and some cancer cell lines and is discordant with hypermethylation of p16; silence of hTERT expression may be more critical in carcinogenesis than that of other genes
- expression may be involved in poor healing caused by sclerosis of small blood vessels and lack of granulation tissue in the neoplastic transformaiton of chronic radiation ulcer
- results suggest that telomerase activity might be associated with the presence of breast cancer cells; telomerase activation may occur early in breast cancer and may be periodically downregulated during subsequent tumor progression
- effect of human telomerase reverse transcriptase (hTERT) gene antisense oligodeoxynucleotide (ASODN) on telomerase activity and apoptosis in lung cancer cell A549 line
- TERT expression did not change after cisplatin treatment for 72 hours.
- Results indicate that in vitro immortalization in HME cells may require the activation of the function of telomerase and other genetic alterations such as the spontaneous loss of p16(INK4a) expression.
- Two distinct pathways of down-regulation of telomerase reverse transcriptase by retinoids co-exist in APL cells.
- High telomerase activity was associated with multiple myeloma
- Increases in hTERT gene copy number is correlated with higher levels of hTERT protein expression in cervical carcinomas with high-risk human papillomavirus infection.
- Human telomerase accelerates growth of lens epithelial cells through regulation of the genes mediating RB/E2F pathway
- after hTERT excision, cells senesce with shorter telomeres than parental cells
- The data show the successful insertion of the hTERT gene into leiomyoma and myometrial cells and the immortalization of these cell lines without phenotypic alteration from the parental cell types.
- TRRAP binding and the recruitment of histone H3 and H4 acetyltransferase activities are required for the transactivation of a silent TERT gene in exponentially growing human fibroblasts by c-Myc or N-Myc protein.
- Keratinocytes engineered to express cdk4(R24C) and hTERT but not p53DD did not exhibit an extended life span.
- Reconstitution of hTERT restores tumorigenicity in melanoma-derived c-Myc low-expressing clones.
- Transfection with hTERT alone immortalized 2 out of 3 bone-marrow-derived EC cultures. Transduction of BMSVTs with hTERT immortalized 4 of 4 cultures, which formed large colonies in vitro and small transient tumours in vivo.
- TERT regulates cell survival independent of telomerase enzymatic activity.
- hTERT is required for full telomerase function.
- Telomerase activation occurs during progression from low to high-grade dysplasia in adenomas & increases with the degree of dysplasia & invasion during colorectal carcinogenesis. hTERT mRNA expression is seen in the late stage development of this cancer.
- Data show that telomerase activity is required to bypass senescence but is not sufficient to prevent telomere erosion and genomic instability at lower levels of expression.
- hTERT expression changed proportionally with the level of telomerase activit & showed differential expression in normal and cancer tissues. hTERT is a regulatable subunit and it has a rate-limiting effect on enzyme activity.
- Data show that although telomerase did not protect cells from stress-induced premature senescence, fibroblasts expressing hTERT were more resistant to stress-induced apoptosis and necrosis.
- Results suggest that Sp1 and Sp3 associate with the hTERT promoter, recruiting HDAC for the localized deacetylation of nucleosomal histones and transcriptional silencing of the hTERT gene in normal human somatic cells
- C-terminal regions of the human telomerase catalytic subunit are essential for in vivo enzyme activity. The C-DAT region is involved in a step of in vivo telomere synthesis after the assembly of a catalytically active enzyme.
- The expression of telomerase may be a crucial step in gastric carcinogenesis and increased hTERT mRNA may serve as a novel marker for diagnosis of GC.
- findings provide evidence for an endogenous, repressive mechanism that actively functions in telomerase/hTERT-negative normal cells and becomes defective during carcinogenic processes
- The levels of anti-hTERT antibodies in serum correlated with progression to hepatocellular carcinoma
- hTERT confers an additional function that is required for tumorigenesis but does not depend on its ability to maintain telomeres
- catalytically active human telomerase has a regulated intranuclear localization that is dependent on the cell-cycle stage, transformation and DNA damage
- TERT mRNA expression a useful marker of clinical aggressiveness of soft tissue tumors
- promotor methylation may be involved in the regulation of human telomerase catalytic subunit, hTERT, gene expression
- Identification of a human telomerase reverse transcriptase peptide of low affinity for HLA A2.1 that induces cytotoxic T lymphocytes and mediates lysis of tumor cells.
- The higher expression level of hTERT mRNA is associated with shorter survival in patients with stage I non-small cell lung cancer.
- hTERT mRNA expression is associated with malignant tumor progression and poor outcome.
- role in expression levels of keratinocyte growth factor ind insulin-like growth factor-II in fibroblasts
- role of transcription factors Sp1 and Sp3 in the regulation of telomerase activity and human telomerase reverse transcriptase (hTERT) in Jurkat T cellls
- HPV-16 facilitates telomerase activation in conjunction with other viral or cellular changes over time
- E2F transcriptional factors have both repressor and transactivator functions in regulating hTERT promoter activity.
- Simian virus 40 small tumor antigen activates AKT and hTERT and induces anchorage-independent growth of human epithelial cells
- Ku associates with hTERT, and this interaction may function to regulate the access of telomerase to telomeric DNA ends
- induction of HO-1 gene mediates protection against oxidants and increases cell survival by a mechanism independent of telomerase enzyme activity
- bone marrow stromal cells were trasfected with the hTERT gene, resulting in increased population doubling and the acquisition of cell immortalization.
- Review. 85% of human tumors have telomerase activity, that in normal cells goes undetected. These characteristics make the telomerase an attractive target for chemotherapy.
- Primary human mammary epithelial cells do not undergo mortality stage 0 growth arrest and can be immortalized without abrogating p16. This supports the concept that hTERT is sufficient to immortalize HMECs when cultured under adequate conditions.
- activation by E1A proteins
- role of expression of dominant-negative mutant on telemere dysfunction and telomerase reactivation
- expression of telomerase genes (hTR, hTRT) and apoptosis related genes (p53, bcl-2) in mammary atypical ductal hyperplasia
- telomerase hTERT gene expression in broncho-alveolar lavage fluid of patients with lung carcinomas
- the first two exons play a major role in the down-regulation of the hTERT promoter in telomerase-positive cells
- Results suggest that up-regulation of telomerase expression play some roles in tarsal gland carcinogenesis, and the expression of hTR be a useful marker for malignant degree of tarsal gland carcinoma.
- human telomerase can send hyperproliferative signals in cells and induce a senescent phenotype
- hTERT was expressed in 88% of ovarian tumors.
- Results describe the relationship between Helicobacter pylori (H.pylori) infection and the expressions of the p53, Rb, c-myc, bcl-2 and hTERT mRNA in a series of diseases from chronic gastritis to gastric cancer.
- Nuclear factor-kappaB p65 mediates tumor necrosis factor alpha-induced nuclear translocation
- Interactions of telomerase with primer substrates are stabilized mainly by contacts with the protein subunit of the enzyme (hTERT).
- association with telomers and role in enhancing genomic stability and DNA repair
- The association between RARbeta and hTERT expression suggests that RARbeta expression may be an indicator of increased risk of lung cancer in heavy smokers.
- IRF-1 is a potential mediator of IFN-gamma-induced attenuation of telomerase activity and hTERT expression
- These results indicate that the highly branched N-glycosylation with augmented galactosylation is induced in human stromal cells immortalized by telomerase reverse transcriptase expression.
- Data show that ectopic expression of human telomerase reverse transcriptase (hTeRT) in human cells leads to an upregulation of the small GTPase Rab9 and its effector p40.
- TERT activity was determined in doxorubicin resistant gastric carcinoma lines.
- Regulation of telomerase activity by interaction with the 90 kDa heat shock protein and by Akt-dependent phosphorylation.
- ectopic hTERT expression extends the life span of CD4+ T helper type 1 or 2 and regulatory T-cell clones and affected neither the in vitro cytokine production profile nor their specificity for antigen
- Amount of hTERT mRNA may represent a diagnostic and prognostic indicator for glioblastoma multiforme patients
- mRNA of this enzyme is a prognostic factor in neuroblastoma.
- results suggest that the endogenous chromatin environment plays a critical role in the regulation of human telomerase reverse transcriptase expression during cellular immortalization
- The gene for this enzyme is deleted and there is a haploinsufficiency of telomere maintenance in Cri du chat syndrome.
- expression of hTRT gene in gastric cancer is correlated significantly to the specific defects of cell cycle on G1/S check point; telomerase activity may depend on cell cycle in gastric cancer
- c-myc binds to the hTERT promoter and is involved in the pathway for regulation of cellular immortalization through BRCA1
- No association observed between grade of the tumour differentiation and semiquantitative levels of hTR- or hTERT-expression.
- It is suggested that the expression of hTERT mRNA leukemic cells indicates their higher proliferation ability.
- Telomerase accelerates osteogenesis of bone marrow stromal stem cells by upregulation of CBFA1, osterix, and osteocalcin.
- a significant correlation between hTERT expression and telomerase activity in skin tumour samples was observed
- telomerase levels in T cells are limiting and increasingly insufficient to sustain their proliferation
- We suggest that this domain posttranscriptionally regulates telomerase function by targeting the enzyme to telomeres
- Blockade of epiregulin reduced the growth of hTERT-BJ cells and colony formation of hTERT-transformed fibroblasts. Moreover, inhibition of epiregulin function in immortal hTERT-BJ cells triggered a senescence program.
- Results demonstrate that mot-2 and telomerase can cooperate in the immortalization process.
- There were three splicing variants in NASG 3'UTR. Its abnormal expression may be an important molecular event in NPC and lung cancer.
- Data indicate that hTERT gene amplification is relatively common in embryonal brain tumors, and that increased expression of hTERT mRNA may be associated with biologically aggressive tumor behavior.
- Detection of this enzyme's mRNA by RTPCR is a sensitive method for the diagnosis of bladder cancer.
- depletion of nuclear TERT by tyrosine phosphorylation-dependent nuclear export of TERT is a novel mechanism for regulation of TERT localization, which reduces the antiapoptotic activity of TERT
- These studies suggest that histone deacetylation is involved in early telomerase reverse transcriptase (hTERT) gene down-regulation and that DNA methylation may maintain silencing of the hTERT gene in these cells.
- E6/Myc interactions regulate hTERT gene expression. The proximal Myc/ Max-binding element (E-box) in the hTERT promoter was the major determinant of both E6 and Myc responsiveness in keratinocytes.
- constitutive overexpression of hTERT does not interfere with epidermal differentiation per se but blocks the terminal stage of differentiation and indicates that hTERT plays an active part in the regulatory pathway of epidermal differentiation.
- Peptide nucleic acids are taken up into human tumor cells when they are components of a peptide fragment of this enzyme.
- Glioblastoma induces vascular endothelial cells to express this enzyme in vitro.
- IGF-I clearly stimulates telomerase activity in prostate cancer cells through a dual mode of action, including early rapid effects probably involving phosphorylation of hTERT by Akt and later up-regulation of hTERT expression.
- The rate-limiting telomerase catalytic subunit hTERT is exprfessed in cycling primary presenescent human fibroblasts.
- Assessment of TERT mRNA expression in urine sediments represents a reliable tool for the detection of primary urothelial neoplasms, equally specific, yet far more sensitive, than conventional cytology.
- Data suggest that the significant difference in clinical behavior of embryonal rhabdomyosarcomas in comparison to other subtypes may be due to differences in telomerase reverse transcriptase expression.
- Expression of telomerase did not reverse the senescent arrest.
- These results uncover further hTERT allele-specific phenotypes that uncouple telomerase activity, net telomere lengthening and life span extension.
- Results describe the immortalization of OSE cells with the catalytic subunit of telomerase (hTERT) and a temperature-sensitive form of SV40 Large T antigen (tsT).
- hTERT is transcriptionally regulated by raloxifene via an estrogen-responsive element-dependent mechanism, which inhibits E2-induced up-regulation of telomerase activity
- expression of telomerase activity and its subunit level in pancreatic carcinoma significantly correlate with the clinical stage of pancreatic carcinoma
- telomerase activation is the early event of carcinogenesis, which is not correlated with clinicopathological factors of hepatocellular carcinoma; oxidative stress may contribute partly to telomerase activation.
- Functional hTERT is expressed in both nucleus and cytoplasm of cancer cells, and hTERT expression does not strictly reflect telomerase activity.
- expression of hTERT is sufficient to immortalize human keratinocytes and does not require inactivation of p16INK4a.
- findings suggest that the acute regulation of hTERT transcription is primarily controlled by E-box elements, which bind a series of factors during the phased phenotypic changes occurring during the differentiation of rhabdomyosarcoma muscle cells
- This implies that rapamycin leads to inhibition of hTERT gene transcription.
- in situ detected TERT mRNA may be useful for diagnosing NSCLC and establishing prognosis
- findings suggest that activation of apoptosis occurs in MDS in the absence of hTERT expression, implicating high apoptosis in the absence of high TA with ineffective hematopoiesis; poor prognosis correlated with higher caspase-3 and lower hTERT rates.
- USF binding to the hTERT promoter may be transcriptionally neutral, or even repressive, in nonimmortalized hTERT-negative somatic cells, but stimulatory in hTERT-positive cells where USF1/2 contributes to the acquisition and maintenance of immortality
- data show that the telosome reacts after DNA damage by upregulating telomerase activity and TRF2 expression in malignant cells
- telomerase is regulated in ovarian tumors by p53 and c-myc
- hypermethylation of the hTERT promoter plays a critical role in the negative regulation of telomerase activity in normal human oral cells
- MNS16A is a polymorphic minisatellite (VNTR) in downstream region of human telomerase gene locus and functionally associated with promoter activity of an antisense RNA transcript, thus may have a role in lung tumorigenesis.
- the shortening of telomeres and reactivation of telomerase occur in dysplastic nodules during early stages of hepatocarcinogenesis, with significant change in transition of grade
- Stress-induced premature senescence in hTERT-expressing ataxia telangiectasia fibroblasts.
- overexpression of heterochromatin protein 1 isoforms HP1(Hsalpha) and HP1(Hsbeta) results in decreased association of a catalytic unit of telomerase (hTERT) with telomeres
- Telomerase activity and hTERT, TP-1 mRNA expression are up-regulated in esophageal squamous cell carcinoma
- an exogenous BRCA1 gene strongly inhibited telomerase reverse transcriptase activity in human prostate and breast cancer cell lines
- possibility of the utilization of the hTERT promoter in targeted cancer gene therapy
- Results suggest that overexpression of hTERT mRNA may be correlated with proliferative activity reflected by Ki-67 immunoreactivity and is an early event in carcinogenesis of the esophagus.
- Haploinsufficiency of TERT leads to telomere dysfunction and radiosensitivity in human cancer cells.
- In patients with advanced renal cell carcinoma, there is a high incidence of telomerase positivity was found, within this limited sample, however, no correlation with survival was found
- NF-kappaBp65 and hTERT expressions are upregulated at the early stage of gastric carcinogenesis
- Epithelial telomerase reverse transcriptase mRNA expression is detected in ovarian carcinomas.
- Analysis of 43 cases of colorectal carcinoma for hTERT mRNA expression and telomerase activity.
- Expression of hTERT in normal fibroblast cell strain resulted in telomerase activity and an extended proliferative lifespan.
- Aurora-A-stimulates hTERT expression and telomerase activity.
- telomerase is downregulated by EGCG in human breast carcinoma cells
- Elevated hTERT mRNA levels is associated with bronchial squamous cell carcinoma
- RTPCR luminescensce is highly sensitive and specific for the detection of hTERT beta-plus transcript in clinical tumor samples.
- c-Myc does not play a major role in gene regulation of the catalytic subunit of telomerase (hTERT) in human hepatocellular carcinoma.
- Catalytic subunit of human telomerase extends the life span of control in Tangier Disease skin fibroblasts.
- Four out of five malignant pheochromocytoma samples were positive for either hTERT expression or Ki-67/MIB-1 immunoreactivity; 9 of 9 unfavorable neuroblastoma samples were positive.
- Human telomerase reverse transcriptase mRNA is highly expressed in normal breast tissues and down-regulated in ductal carcinoma in situ
- Telomerase expression could be a marker indicating the malignant potential of prostate cancer
- TERT was tracked in telomere synthesis.
- Data show that a DAT domain mutant of hTERT is efficiently rescued upon fusion to hPot1.
- hTERT/telomerase accelerates DNA repair by recruiting DNA repair proteins to the damaged DNA sites
- These data provide novel insight into the catalytic organization of the human telomerase complex and suggest that repeat addition processivity is one of the major catalytic properties conferred by telomerase multimerization.
- REVIEW: mechanistic insight into how oncogenic activation as well as derepression, often due to the inactivation of tumor suppressors, stimulate the hTERT promoter
- Unlike Tetrahymena telomerase, human telomerase catalyzed the removal of more than one nucleotide (up to 13) from telomeric primers
- hTERT methylation is a function of age and is associated with a poorer outcome in ovarian and cervical cancer, irrespective of hTERT expression.
- Results suggest that changes in telomerase activity and K-ras point mutation at codon 12 may be an early event of malignant progression in pancreatic cancer.
- hTERT localized diffusely in the nucleoplasm and more intensely in the nucleoli of cancer cells and proliferating normal cells. Mitotic cells showed diffuse staining of the entire cell. hTERT negative tumor cells were identified as apoptotic cells.
- telomerase activity (TA) in CLL bone marrow correlated significantly with leukocyte and lymphocyte count but not with bone marrow cellularity, beta2-microglobulin, nor other patient characteristics; there was no correlation between TA and survival
- Close relationship between hTERT transcription and neuroblastoma proliferation.
- telomerase reverse transcriptase has a role in the interaction of KIP with telomerase
- hTERT mRNA and telomerase activity increased sharply during passage of UT-DEC-1 cells containing integrated human Papillomaivrus type 33.
- regulation of hTERT promoter activity by HIF-1alpha represents a mechanism for trophoblast growth during hypoxia and suggests that this may be a generalized response to hypoxia in various human disorders
- hTERT/rev-caspase-6 exhibited a greater ability to induce apoptosis in malignant glioma cells than /caspase-6.
- A specific immune response exists against hTERT in breast cancer patients.
- A lymphatic endothelial cell line transfected with TERT was found to have an extended life span.
- Transient transfection assays revealed that Tax stimulates the hTERT promoter through the nuclear factor kappaB (NF-kappaB) pathway
- dynamic assembly of E2F-pocket protein-HDAC complex plays a central role in the regulation of hTERT in a variety of proliferative conditions (e.g., normal cycling, senescent, and tumor cells).
- Higher expression of human telomerase reverse transcriptase may be used as new parameters for evaluating the degree of malignancy of retinoblastoma.
- hTERT-transduced MRC5 fetal lung fibroblasts (MRC5hTERTs) bypassed senescence but but eventually succumbed to a second mortality barrier (crisis)
- catalytic subunit of telomerase (hTERT) protects a maturation-resistant acute promyelocytic leukemia cell line from apoptosis induced by the tumor necrosis factor (TNF) or TNF-related apoptosis-inducing ligand
- results suggest that the interaction of KSHV LANA with Sp1 up-regulates the telomerase promoter, potentially contributing to the immortalization of KSHV-infected cells
- Bisphenol A from dental crowns increased the expression level of hTERT mRNA in a cell line.
- the induction of hTERT by the HPV-16 E6/E6-AP complex involves targeting of NFX1-91, a newly identified repressor of telomerase, for ubiquitination and degradation.
- interaction of hTERT and nucleolin participates in the dynamic intracellular localization of telomerase complex
- repression of hTERT by endogenous p53 is mediated by p21 and E2F
- in vitro PinX1 binds directly to the hTERT protein subunit, primarily to the hTR-binding domain, as well as to the hTR subunit
- Genomic instability is associated with lack of telomerase activation in ovarian cancer
- down-regulation of telomerase activity by 1,25(OH)(2)VD(3) and the resulting cell death are important components of the response of OCa cells to 1,25(OH)(2)VD(3)-induced growth suppression
- These results suggest that hTERT expression occurs preferentially in malignant tumors and that telomerase activity may occur during the progression of GISTs.
- hTERT is embedded in a nuclease-resistant chromatin domain
- TERT-expressing CD8+ T cells show enhanced inhibition of HIV-1 replication in vitro.
- p53, bcl-2 and telomerase have roles in progression of Egyptian breast cancer patients
- The human telomerase reverse transcriptase (hTERT) is targeted to the mitochondria by an N-terminal leader sequence, and that mitochondrial extracts contain telomerase activity which may sensitize cells to oxidative stress.
- LMP1 activates telomerase via c-myc
- hTERT has antiapoptotic activity and its p53-mediated downregulation is critical for efficient p53-dependent apoptosis.
- hTERT levels are significantly high in childhood acute lymphoblastic leukemia with the highest level of pre-B cell leukemia before treatment
- The hTERT promoter may be suppressed by the 5'-flanking elements and IL-2 may signal for de-suppression in association with promotion of cell growth in T cells.
- c-myc and mad1 can regulate the hTERT transcript in a different manner in hTERT positive cells, but not in normal cells
- E6AP ubiquitin ligase is required for transactivation of the hTERT promoter by the human papillomavirus E6 oncoprotein
- Ki-67 and hTERT have roles in meningioma cell proliferation, but only hTERT has a role in disease recurrence
- p27Kip1 inhibits hTERT mRNA expression and telomerase activity through post-transcriptional up-regulation by IFN-gamma/IRF-1 signaling
- overexpression of hTERT in CD4+ T cells provides a proliferative advantage independent of the average telomere length but does not prevent eventual genetic instability and replicative senescence
- Hepatitis X protein up-regulates the expression and activity of hTERT in hepatoma cells, suggesting that hTERT is associated with tumor development.
- Functional characterization of hTERT-immortalized CRC cell lines shows that they have a transformed phenotype, being able to form colonies in soft agar and tumors in severe combined immunodeficient mice.
- High expression of hTERT is associated with pancreatic cancer
- depending on its location relative to the DNA 3'-end, protection of telomeres 1 protein (POT1) can either inhibit telomerase action or form a preferred substrate for telomerase
- Results suggest a possible mechanism for pseudoknot formation based on the dynamics of the hairpin structure and also may explain the mutational aspects of dyskeratosis congenita.
- Human mesenchymal stem cells (MSC)-TERT may constitute a valuable tool for mechanistic studies on how to control MSC homing and engraftment
- Heterozygous mutations in the TERT gene impair telomerase activity by haploinsufficiency and may be risk factors for marrow failure.
- telomerase activity contributes to anaplastic transformation of differentiated carcinoma
- Survivin up-regulates telomerase activity by augmenting gene transcription of hTERT.
- Results indicate that telomerase is repressed in most lung carcinoids and that hTERT transcription and alternative splicing play a role in such a negative regulation.
- hTERT can suppress RAS/RAF/MEK/ERK signaling pathway to prevent differentiation
- RID1 (RNA interaction domain 1) and the hTERT C terminus contribute to telomerase's affinity for its substrate, and RID1 may form part of the human telomerase anchor site
- Serum hTERT mRNA is a novel and available marker for hepatocellular carcinoma diagnosis.
- DNA mutational analysis reveals first natural mutations of TERT to be described; their possible pathogenic role in the development of bone marrow failure is discussed
- The most accurate telomerase-based test for bladder cancer detection with the potential to replace cytology as a noninvasive biomarker for disease diagnosis and follow-up.
- suppression of the telomerase catalytic subunit hTERT expression abrogates the cellular response to DNA double strand breaks
- In the presence of manganese, both yeast and human telomerase can switch to a template- and RNA-independent mode of DNA synthesis, acting in effect as a terminal transferase.
- hTERT-transduced CD8+ cytotoxic T lymphocytes clone showed a decreased functional activity on prolonged culture
- upstream stimulatory factor lose the inhibitory effect on hTERT expression leading to telomerase reactivation and oral carcinogenesis
- Negative regulation of the hTERT gene may represent one mechanism by which tumor necrosis factor-alpha interferes with normal hemopoiesis
- CtBP is a potential repressor of hTERT and hTERC
- additional role for POT1 in telomere maintenance: disrupting G-quadruplex structures in telomeric DNA, thereby allowing proper elongation by telomerase
- hTERT mRNA partially regulates telomerase activity in normal gastric mucosa and gastric adenocarcinoma. In contrast, hTERT mRNA splicing is not involved in the regulation of enzyme activity.
- telomerase RNA template sequence is a key determinant of the contribution of telomerase to telomere length regulation
- Estrogen reduces endothelial stem cell senescence through augmentation of TERT.
- Overlapping roles are identified for TR sequences and structures 5' of the template in regulating both 5' template boundary definition and 5' template usage
- Overexpression of AP-1 leads to transcriptional suppression of hTERT in cancer cells; it directly regulates the hTERT promoter.(TERT)
- hTERT and hTR are subject to similar controls under hypoxia
- Telomerase overexpression does not prevent proliferation-associated telomere shortening in human hematopoietic cells.
- High levels of telomerase mrna is associated with high grade breast neoplasms
- Inhibition of histone deacetylation selectively regulates the transcriptional derepression of telomerase catalytic subunit in normal lung fibroblast cells compared to lung tumor cells.
- the p73 gene functions as an important regulator of TERT activity
- overexpression of C-terminal p73 isoforms (alpha, beta, gamma, delta) resulted in a clear down-regulation of human telomerase reverse transcriptase (hTERT) promoter activity
- HBx gene transfection can upregulate the transcriptional expression of hTERT mRNA; transactivation of hTERT gene by HBx gene is a newfound mechanism for pathogenesis of hepatocarcinomas and cholangiocarcinomas after HBV infection.
- We detected an interaction between E2F1 protein and the hTERT promoter. E2F1 mRNA expression and hTERT mRNA expression were statistically significantly correlated in human glioblastoma specimens.
- The abnormal expression of telomerase in hepatoma could be a useful marker to the diagnosis and prognosis of this disease.
- TGF-beta1 exerts opposing effects on telomerase activity in anaplastic thyroid carcinoma cell lines
- The interaction of CCCTC-binding factor with the first exon of the hTERT gene represses hTert gene expression.
- GC-box-mediated, human-specific mechanism for TERT repression is impaired in human cancers
- These results define H3 phosphorylation as a key to hTERT transactivation induced by proliferation and reveal a fundamental mechanism for telomerase regulation in both normal human cells and transformed T cells.
- Expression of hTERT dropped in testes with postmeiotic spermatogenic arrest. At least low levels of hTERT were detectable in testes with premeiotic spermatogenic arrest.
- immunological identification of the nature of telomerase positive cells
- differential regulation of hTERT and vascular endothelial growth factor in p63-positive breast carcinomas may contribute to the clinically more aggressive behavior of these neoplasms
- this study present evidence for a molecular link between cholesterol-activated Receptor Ck and hTERT transcription, and provide new insights into the regulation of hTERT expression in normal human peripheral blood mononuclear cells.
- These findings suggest that the functional (-1327)T/C polymorphism of hTERT is associated with leukocyte telomere length in normal individuals.
- A significant correlation was observed between human papilloma virus-16 viral load and quantitative hTERT mRNA expression in women with high-grade cervical dysplasia.
- the type of genomic instability in human cells may depend critically on whether they are telomerase-positive or -negative
- one potential mechanism whereby IFNs induce apoptosis sensitization is by repressing hTERT transcription and telomerase activity, thereby constituting attractive targets for multiple myeloma therapy.
- hTERT mRNA is a hallmark of malignant adrenocortical tumors, but identifies also a subset of hTERT-expressing APAs that might show metastatic spread at long-term follow-up
- Data suggests that telomerase is an important prognostic indicator of survival in patients with brain tumors.
- shRNA directed against hTERT inhibits telomerase activity through suppression of the hTERT expression in laryngeal cancer cells and that RNA interfering technology may be a promising strategy for the treatment of laryngeal cancers.
- The sequences in the human carboxy-terminal domain of TERT are critical for telomere maintenance in human fibroblasts
- An increase in telomerase activity in normal bronchial epithelium might extend the lifespan of cells at risk for malignant transformation, and thus contribute to lung carcinogenesis.
- Alterations in the level of the full-length variant of hTERT showed different gene expression profiles and genomic copy number changes in breast cancer.
- Senescence of a Grade III derived meningioma cell line was overcome by expression of the telomerase catalytic subunit (hTERT).
- The functional VNTR MNS16A in downstream region of human telomerase gene locus is a predictor for survival in patients with glioblastoma multiforme.
- localization of hTERT to the mitochondria renders cells more susceptible to oxidative stress-induced mtDNA damage and subsequent cell death
- nuclear hTERT immunostaining patterns may reflect the functional status of non-neoplastic brain and neoplastic astrocytic cells
- Expression of hTERT bypassed Rb and p53 pathway-dependent barriers to proliferation and immortalised normal human urothelial cells
- hTERT is an endogenous inhibitor of the mitochondrial cell death pathway
- hTERT gene amplification and mRNA overexpression is associated with non-small-cell lung cancer
- novel pro-survival role for hTERT in hematopoietic stem cells and implications for the role of hTERT in the pathogenesis of leukemia and drug resistance
- Advanced age as well as high telomerase activity and TERT mRNA levels were seen to be significant predictors of worse prognosis in glial tumors.
- activation of NF-kappaB was observed in immortalized nasopharyngeal 460hTert cells at the later population doublings, and may play a role in the survival of immortalized nasopharyngeal epithelial cells
- immortalization by HPV results in upregulation of hTERT and furthers our understanding of how telomerase is activated during the process of malignant transformation
- CTL activity induced by plasmid based DNA vaccine-transfected PBMCs specifically lysed a variety of human leukocyte antigen-matched and hTERT-positive human tumor cells
- hsp90 destabilases the hTERT polypeptide.
- Expression of hTERT mRNA correlated with the progression of stage and grade in bladder cancer.
- CROSS-HYBRIDIZATION WITH NUCLEOLIN WHEN DETECTING TERT WITH IMMUNOPROBE CURRENTLY AVAILABLE.
- TGFbeta suppresses TERT by Smad3 interactions with c-Myc and the hTERT gene
- A high level of hTERT mRNA was observed in the anaplastic thyroid cancer cell line OCUT-1, correspinding to increased expression of telomerase due to accelerated telomere shortening.
- human atherosclerosis is characterized by senescence of VSMCs, accelerated by oxidative stress-induced DNA damage, inhibition of telomerase and marked telomere shortening.
- Telomerase antisense oligodeoxy-nucleotide can inhibit the proliferation of pancreatic cancer cell line Bxpc-3 and decrease the telomerase activity and increase cell apoptosis rate in vitro.
- These results indicate that the detection of circulating hTERT mRNA was identified in almost all cholangiocarcinoma patients. It offers a novel tumor marker, which can be used as a complementary study for diagnosis of cholangiocarcinoma.
- hTERT has a novel role in the modulation of cyclin D1 expression
- up-regulation of hTERT expression within the cord blood hematopoietic stem cell pool is accompanied by decreased self-renewal capacity.
- hTERT/RGD-alpha3(IV)NC1 gene therapy showed limited expression of RGD-alpha3(IV)NC1 in tumors and resulted in a significant decrease of vessel density in tumors in nude mice.
- higher frequency of 1327C/C genotype in CAD patients (51.9%) compared with controls (36.5%). Among 104 CAD patients, leukocyte telomere length in the -1327C/C genotype (7.62+/-2.19 kb) was shorter than that in -1327T/C & -1327T/T genotypes (8.74+/-2.92).
- high-risk human papillomaviruses were involved in inducing p16 and hTERT overexpression in Bowenoid papulosis
- telomerase expression is altered in hepatocellular carcinoma
- Ten different alternatively spliced sites were detected in hTERT and the majority of hTERT cDNA clones from normal and tumour lung and colon tissues encoded truncated proteins ending close after exon 2 or 6.
- Early passage T cell clones transduced or not with human telomerase reverse transcriptase display identical growth rates upon mitogenic stimulation and no marked global changes in gene expression.
- These results show for the first time that IP6 represses telomerase activity in prostate cancer cells by posttranslational modification of TERT via the deactivation of Akt and PKCalpha.
- Here, we provide functional characterization of an additional 8 distinct hTERT sequence variants and 5 hTERC variants that have recently been identified in patients with dyskeratosis congenita (DC) or aplastic anemia (AA).
- Up-regulation of hTERT was closely associated with high-risk human papillomavirus, due to activation by the E6 oncoprotein.
- The expression of hTERT mRNA may play a role in pathogenesis in pulmonary neuroendocrine tumors, and be a useful tool for differential diagnosis between typical carcinoids and large cell neuroendocrine carcinoma.
- Telomerase activity may be used as an adjunct to cytology and HPV DNA testing in triaging women with cervical lesions.
- This study showed the overexpression of hTERT mRNA and telomerase activity in the endometrium of endometriosis patients. These finding suggest that replication potential of endometrial cells may have an important role in the pathogenesis of endometriosis
- hTERT may be involved in a feedback loop system, thereby playing a role in its own regulation.
- protein kinase C theta-activated NFkappaB signaling regulates the expression of hTERT via cMyc in human T lymphocytes
- Human tumor xenograft in nude mice is suppressed significantly by the treatment with a hTERT/re-Caspase-3 system.
- h-TERT mRNA may be a predictive value for recurrence of hepatocellular carcinoma in living donor liver transplantation.
- Antisense oligonucleotides of hTERT effectively inhibit the growth of endometrial cancer cell line
- the effect of calcium on telomerase in the human epidermal keratinocyte line HaCaT showed calcium directly interacts with the telomerase complex. This interaction could be mediated by the calcium-binding protein S100A8
- By comparing the expression of p53, cyclin D1, p16, hTERT, and TSP-1 in spontaneously regressing keratoacanthoma and squamous cell carcinoma, the changes in the expression of these proteins to specific stages of skin carcinogenesis, is defined.
- Results collectively showed that normal and malignant lymphocytes express splicing variants of hTERT mRNA and require transcriptional activation of the hTERT gene to acquire telomerase activity.
- Data show that Oct-4, Rex-1, and Gata-4 expression in human mesenchymal stem cells increases cell differentiation efficiency but not hTERT expression.
- many human cell types undergo senescence by activation of the p16(INK4a)/Rb pathway, and that introduction of Bmi-1 can inhibit p16(INK4a) expression and extend the life span of human epithelial cells
- Substitutions at Val867 in human telomerase reverse transcriptase lead to significant changes in overall enzyme activity and telomere repeat extension rate, but have little effect on polymerase processivity.
- hTERT methylation prevents binding of the CTCF repressor, but partial hypomethylation of the core promoter is necessary for hTERT expression.
- data suggest that NFX1-123 is integral to hTERT regulation in HPV16 E6-expressing epithelial cells and that the interaction between NFX1-123 and poly(A) binding proteins is critical to hTERT activity
- The TERT-immortalized hMSCs showed higher stability at telomeric regions than primary hMSCs indicating that cells with long telomeres and high telomerase activity have the advantage of re-establishing the telomeric caps.
- These data suggest that hTERT contains distinct anchor regions that cooperate to help regulate telomerase-mediated DNA recognition and elongation.
- repression mechanisms of the TERT gene is triggered by TAK1
- study identifies hTERT-RNA level as a new prognostic marker in B-cell chronic lymphocytic leukemia, and may be used to identify previously unrecognized patient groups with the same IgVH mutation status and different disease outcomes
- expression of hTERT reduces the number of foci and the level of active p53, thereby decreasing sensitivity to growth factor depletion
- Porcine ventricular endocardial endothelial cells were immortalized by transfecting them with TERT.
- REVIEW: control of telomerase activity at chromosome ends by telomere-binding proteins
- identification of two complexes of telomerase of molecular masses 680 & 380 kDa, both of which retain telomerase activity in vitro; findings show that the former complex does not include Hsp90; the latter contains Hsp90
- The results indicate that telomerase alterations have an important role in the pathogenesis of BCCs. A unique finding is that the telomerase expression level in nodular BCCs is different from that in superficial BCCs.
- Mutations in the genes encoding telomerase components can appear as familial idiopathic pulmonary fibrosis. Our findings support the idea that pathways leading to telomere shortening are involved in the pathogenesis of this disease.
- protein composition of catalytically active telomerase; mass spectrometric sequencing of the components & molecular size determination indicate an enzyme composition of two molecules each of telomerase reverse transcriptase, telomerase RNA & dyskerin
- the short allele of MNS16A of the hTERT gene is more frequent in glioma patients, but it did not seem to be predictive of survival.
- These findings indicate that hTERT contributes by multiple mechanisms to the EBV-driven transformation of B lymphocytes and suggest that hTERT may constitute a therapeutic target for EBV-associated B cell lymphomas.
- telomerase limits the accumulation of telomere dysfunction, the evolution of excessive aneuploidy, and the activation of p53-independent checkpoints suppressing hepatocarcinogenesis.
- mutations in TERT or RNA component of telomerase that result in telomere shortening over time confer a dramatic increase in susceptibility to adult-onset Idiopathic pulmonary fibrosis
- stress-induced premature senescence (SIPS) from hydrogen peroxide is associated with a transient increase in DNA-binding activity of p53 and an increased expression of p21(WAF-1) in human hTERT fibroblasts
- To our knowledge, this represents the first report on collocating a low-affinity epitope variant with a full-length hTERT gene for anti-cancer vaccine design.
- Repression of hTERT expression in human mesenchymal stem cells is due to promoter-specific histone hypoacetylation coupled with low Pol II and TFIIB trafficking.
- No increased age-related attrition was observed for the (-1327)C/C genotype as previously indicated, rather a telomere elongation in the control group (p=0.021) not seen in the MI group (p=0.249).
- The anti-cancer effects of Gleditsia sinensis extract on esophageal squamous cell carcinoma involve the suppression of oncogenic expression and inhibition of telomerase activity.
- Review. Telonmerase(-) human breast epithelial cells show greater p53 stability and more growth arrest to various stimuli. Telomerase abrogates this and elongates short telomeres.
- PLZF upregulates apoptosis-inducer TP53INP1, ID1, and ID3 genes, and downregulates the apoptosis-inhibitor TERT gene
- Data show that increased generation of C18-ceramide by hCerS1 expression mediates the association and recruitment of deacetylated Sp3/HDAC1 to the hTERT promoter, resulting in local histone H3 deacetylation and repression of the promoter.
- These findings indicate that a telomere length pattern does exist in mesenchymal stem cells and that the pattern is not actively re-established after destruction by irradiation.
- hTERT expression in premalignant cervical lesions is not caused by DNA methylation
- transfection of the hTERT gene in immortalized pleomorphic adenoma does not alter the nature of those cells
- Telomerase activity is elevated in nasal polyps but that does not predict the recurrence of nasal polyposis after surgical treatment.
- mutations in the telomerase complex and their connections with dyskeratosis congenita and bone marrow failures [review]
- activating enhancer-binding protein-2beta (AP-2beta) as a novel transcription factor that specifically binds to and activates the hTERT promoter in human lung cancer cells.
- Elevated expression of cell-cycle regulators p16(INK4A), p21(CIP1), and cytoplasmic/nuclear beta-catenin correlated with increased colorectal cancers risk, as did elevated expression of survivin and human telomerase reverse transcriptase.
- Increased hTERT expression between carcinoma in itu and high grade carcinoma in situ exhibits progression in cervix cancer.
- Both MYC amplification and TERT expression appear to be associated with high genomic instability and proliferation.
- no link between telomerase activity and microsatellite instability status; patients with telomerase negative tumors had better overall survival
- hTERT and extremely low concentrations of TGF-beta1 (2 pg/mL) synergistically activate ERK 1/2 in fibroblasts by a mechanism that is independent of the autocrine TGF-beta1 loop
- TERT mRNA did not show a clear difference between anaplastic thyroid carinoma cells and other thyroid tumors.
- an increased pattern of hTERT expression according to the malignant progression of colorectal carcinoma was seen.
- our hypothesis is that when the TRF length becomes shorter during tumour progression, the tumour cells can sustain a better tolerance to shorter telomere with the help of both TRF1 and TRF2, but without immediate activation of the telomerase
- These results indicate that the c-Myc/Max b-HLH-LZ dimer binds specifically and equally to both E-box sites of the hTERT promoter and induces a significant bending of the promoter.
- Antisense oligonucleotides can significantly inhibit telomerase activity by downregulating the hTERT mRNA and protein expression, and inhibition of telomerase.
- hTERT appears to be efficiently regulated by EPO and TGFbeta in an opposite way in erythropoietic cells, arguing for a role of telomerase in red blood cell production
- Oncogenic viruses like HTLV-I have evolved a wide repertoire of strategies to stimulate telomerase functions at the transcriptional and post-transcriptional levels
- homozygous TERT mutations, resulting in a pure but severe telomerase deficiency, produce a phenotype of classical AR-DC and its severe variant, the HH syndrome
- hTERT may represent a tool for the generation of tissue engineered chondrocytes suitable for autologous re-implantation into the affected areas of osteoarthritic joints.
- reduction of hTERT activity in CAPE treated CCRF-CEM cells was more prominent in the initial 48 h
- human papillomavirus 18 E6 protein sensitizes HeLa cells to Herpes simplex virus-dependent apoptosis through hTERT and p53.
- study concludes that a TERT gene deletion leads to slightly shorter telomeres within 1 generation; however, results suggest several generations of TERT haploinsufficiency are needed to produce the very short telomeres in dyskeratosis congenita patients
- Data suggest that cultured rheumatoid arthritis fibroblast-like synoviocytes are altered cells and display a distinct gene expression pattern.
- binding to E6AP is not necessary for E6 localization to or activation of the hTERT promoter and another activity of E6 is involved in hTERT activation.
- Up-regulated human telomerase reverse transcriptase expression was detectable and increased gradually with cancer development and was primarily observed at the borderline IPMN stage
- hTERT mRNA could be a useful marker of tumor progression for the early diagnosis and prognosis of bladder cancers.
- The functional interplay between EGFR overexpression, hTERT activation, and p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induces tumor development, invasion, and differentiation.
- Resistance to Ara-c up-regulates telomerase activity.
- The androgen-mediated repression of hTERT is abrogated in a human prostate cancer cell line exhibiting hormone-dependent growth, which expresses a mutant androgen receptor(T877A) frequently occurring in prostate cancer
- analysis of coupled down-regulation of mTOR and telomerase activity during fluorouracil-induced apoptosis of hepatocarcinoma ce
- The work on cell lines can be repeated on tissues utilizing hTERT as the therapeutic target for demethylation using 5azadC in glioma.
- data demonstrate that telomerase upregulation by the endothelial cells is a key requisite for GBM tumor angiogenesis
- The role of the telomerase-specific motif in the telomerase activity and the TERT-telomerase RNA interaction was studied.
- Full-length hTERT transcript was present in both normal/malignant gastric tissues, precluding its use as a gastric cancer marker. Full-length hTERT mRNA expression may indicate a progressive gastric cancer.
- Colorectal adenocarcinoma revealed expression of telomerase hTERT mRNA, which was detected quantitatively by real-time PCR. hTERT could be a potential biomarker for colorectal cancer.
- at the TERT gene locus in human tumour cells containing a functional SWI/SNF complex, Brm, and possibly BRG1, in concert with p54(nrb), would initiate efficient transcription and could be involved in the subsequent splicing of TERT transcripts
- in these families the expression of both TERT alleles and the inherited telomere length contribute to the clinical phenotype
- This review describes the current understanding of telomerase in humans, with particular focus on telomerase biogenesis and regulation in its cellular context.
- These data demonstrate that this domain of dyskerin plays an important role in telomerase maintenance following cell insults such as cisplatin treatment, and in telomerase-defective cells in patients with X-linked dyskeratosis congenita.
- Chromosomal analyses revealed that hTERT probably inhibited the in vivo prostatic tumorigenicity by maintaining genomic stability.
- hTERT-targeted RNA interference inhibits tumorigenicity and motility of HCT116 cells.
- Telomerase activity in aspirates collected through transthoracic fine-needle biopsy aspirates of primary peripheral non-small cell lung cancer could be a helpful independent prognostic factor
- Telomerase activity and expression level of TERT are potential markers for high grade malignancy in NF1 patients
- Study demonstrates using co-transfection assays that HTLV-1 HBZ in association with JunD activates the hTERT promoter.
- Functional link between MYC and hTERT seems to be impaired depending on the molecular essential thrombocytthemia subtype.
- Inhibition of SIRT1 in telomerase-immortalized human cells and hematopoietic stem cells obtained from SIRT1-deficient mice enhanced cell growth under normal and nutrient limiting conditions.
- hTERT transcription repression by LSD1 occurs via demethylating H3-K4 in normal and cancerous cells, and together with HDACs, participates in the establishment of a stable repression state of the hTERT gene in normal or differentiated malignant cells
- loss of miR-138 expression may partially contribute to the gain of telomerase reverse transcriptase protein expression in anaplastic thyroid carcinoma.
- Downregulation of telomerase reverse transcriptase (TERT), caused loss of pluripotency and human embryonic stem cell differentiation to extraembryonic and embryonic cell lineages.
- reverse transcriptase expression may predict recurrence in giant-cell tumor
- exposure of human T lymphocytes to cortisol is associated with a significant reduction in telomerase activity both during primary stimulation of resting cells and secondary stimulation of previously activated cells
- telomerase activity-independent TERT function may contribute to cancer development and aging independently of telomere lengthening.
- There may be a relationship between cyclosporine-induced gingival overgrowth and telomerase activiy.
- telomerase activity is regulated by at least two mechanisms during granulocytic and monocytic differentiation in a tumor cell line.
- VEGF, hTERT and Bcl-xl have roles in laryngeal squamous carcinoma
- Genome-wide, high-resolution array-CGH was used to screen for genomic aberrations in endometriosis.
- Epigenetic changes in the hTERT promoter represent a stable locking mechanism in the retinoid-induced suppression of telomerase activity.
- hTERT (telomerase reverse transcriptase) mRNA value is a significant factor for childhood acute lymphoblastic leukemia at diagnosis in relation to estimated survival.
- These findings provide a profile of telomere regulation by cell cycle dependent expression of telomerase in hMSCs and may lead to a better understanding of the stem cell nature of these cells.
- Incresed expression of p16(INK4a) and telomeres shortening were observed with age in some stem and progenitor cells.
- High hTERT-mRNA expression is associated with Barrett carcinoma
- Telomerase reconstitution increased fibroblast migration through activation of CXCL12/CXCR4 axis and Rho family.
- The human TERT gene-induces immortalization prolongs the life span of minipig mesenchymal stem cells.
- Human TERT gene has been transfected into mesenchymal stem cells from bone marrow of rhesus monkeys.
- Telomerase activation probably takes place before a cancer phenotype develops and has prognostic significance for survival of patients with laryngeal carcinoma.
- The response of rhythmic gene expression to serum stimulation was markedly attenuated in senescent fibroblasts, telomerase-reconstituted fibroblasts reset the circadian oscillation of rhythmic gene expression.
- hTERT gene-based drug design may be useful in the treatment of leukemic myelopoiesis.
- the MNS16A variant in the hTERT gene may contribute to the risk of breast cancer development and metastasis in Chinese women
- Antisense technology targeted hTERT strategy might be a potential approach for gastric cancer therapy.
- Report effect of antisense-mediated inhibition of survivin, hTERT and VEGF in bladder cancers.
- While hTERT transcription correlated directly to telomerase protein level and activity, as well as longer telomeres in the young group, such correlations were missing in the older group.
- hTERT is one of the important proteins in the proliferation-promoting effect of VEGF on tumor cells in prostate cancer
- Correlations of BMI-1 expression and telomerase activity in ovarian cancer tissues.
- Some hTERT-transduced normal cells may express high levels of the transgene but fail to up-regulate endogenous hTR expression sufficiently to enable expression of robust levels of telomerase activity.
- Inactivation of telomerase before combined hyperthermia and radiotherapy could improve tumor killing.
- Egr-1 plays an important regulatory role in the transcriptional activation of hTERT.
- TERT mutations have a role in autosomal dominant dyskeratosis congenita
- severe anemia with low Hb concentration might up regulate hTERT expression of bone marrow CD34+ cells and serum erythropoietin levels in patients with beta-thalassemia major
- These data demonstrate that targeted degradation of NFX1-91 by E6/E6AP dissociates the mSin3A/HDAC complex from the hTERT promoter and induces hTERT transcription.
- Activation of the PI3K pathway mediated cytoplasmic retention of the Wilms tumor (WTI) protein, which strongly suppressed the hTERT promoter.
- a combinatorial eNOS/ERalpha complex at the hTERT estrogen response element site and that active eNOS and ligand-activated ERs cooperate in regulating hTERT expression in the endothelium.
- residues 965-981 of the human TERT polypeptide constitute an active nucleolar-targeting signal (NTS) essential for mediating human TERT nucleolar localization
- The combination of rAAV-hTERTC27 and a therapeutic dose of rAdv-hTERTC27 is potentially a promising treatment for glioblastoma.
- findings show that CpG island methylator phenotype leads to high levels of expression of telomerase activity in hepatocellular carcinoma through the simultaneous inactivation of multiple genes associated with telomerase activity by concordant methylation
- the trafficking of telomerase to Cajal bodies and telomeres in cancer cells correlates with and depends on the assembly of the enzyme
- tissue samples obtained from patients with hepatocellular carcinoma and cholangiocarcinoma were positive for telomerase reverse transcriptase
- Telomerase inhibition decreased telomere length and reduced growth capacity of telomerase positive chondrosarcoma cells.
- an almost two-fold increase in the number of telomerase-positive tumor cells in uterine myomas compared with myometrial cells 3-4 cm from the tumor
- hTERT gene expression is maintained by a mechanism involving Ets2 interactions with the c-Myc transcription factor and the hTERT gene promoter, a protein-DNA complex critical for hTERT gene expression and breast cancer cell proliferation.
- Differential expression of proteins in human TERT in human mesenchymal stem cells provide an insight into lack of transforming activity of hTERT during cell proliferation.
- PAX8 binds directly to the hTERT and hTR promoters, up-regulating hTERT and hTR mRNA, as well as telomerase activity
- 6 new missense mutations in TERT were identified in subjects with adult-onset pulmonary fibrosis
- Activation of the hTERT expression in squamous cell cervical carcinoma is not associated with gene amplification.
- These data supports the concept of both telomere-based and non-telomere effects of telomerase
- Patients with familial papillary thyroid cancer display an imbalance of the telomere-telomerase complex in the peripheral blood, characterized by short telomeres, hTERT gene amplification, and expression.
- Among the 16 tumors with p14(ARF) hypermethylation, all 12 tumors lacking p14(ARF) mRNA were positive for hTERT expression, while only one of 4 tumors expressing p14(ARF) mRNA was positive for hTERT expression
- These findings indicate that LMP1 simultaneously modulates multiple signal transduction pathways in B cells to transactivate the hTERT promoter and enhance telomerase activity, thus confirming the pleiotropic nature of this viral oncoprotein.
- telomerase reactivation and maintenance of telomeric repeats appear necessary for childhood ependymoma progression
- These data challenge previous findings outlining hTERT's negative impact on overall survival
- These findings indicate that the activation of hTERT expression and telomerase activity by estradiol in human mesenchymal stem cells depends on ERalpha, but not on ERbeta.
- immunostaining for telomerase was significantly & differentially increased in various endometrial cellular compartments in women with recurrent reproductive failure (P < 0.05). There were no significant differences in mean telomere length between groups
- This study suggests that telomerase can prevent genomic instability caused by Cr (VI), but not by radiation.
- The levels of hTERT mRNA were significantly higher in malignant cases compared to those in non-malignant samples.
- examined the relationship of exercise energy expenditure (EEE) with both telomere length and telomerase activity in addition to accounting for hTERT C-1327T promoter genotype.
- A role for the hTERT beta deletion variant in the negative regulation of enzyme activity.
- Malignant thyroid tumors exhibited a greater proportion of the active full-length hTERT transcript (0.57 +/- 0.15) than inactive splice variants, alpha(-) (0.13 +/- 0.02), or beta(-)/alpha(-)beta(-) deletion transcripts (0.30 +/- 0.11; p < 0.001).
- requirement for Myc in the induction of the hTERT promoter by E6 and suggested that occupancy of the promoter by Myc determines the responsiveness of E6 and the downstream induction of telomerase and cell immortalization.
- physical interaction between hsp90 and the hTERT promoter occurs in telomerase-positive cells but not in normal human cells and is necessary for the enhanced hTERT expression and telomerase activity in cancer cells
- hTERT expression was up-regulated in higher stages of urothelial carcinoma.
- Shp-2 retains TERT in the nucleus by regulating tyrosine 707 phosphorylation.
- Genetic variation of TERT is associated with susceptibility to idiopathic pulmonary fibrosis.
- Data show that hTERT activity or inactivation of p53 can suppress the cell proliferation defects associated with lamin A mutants that are incorrectly processed.
- Diploid human fibroblasts in which hPOT1 expression has been suppressed harbor telomeres that are longer than control cells.
- TERC and TERT gene mutations in patients with bone marrow failure and the significance of telomere length measurements
- The epithelial expression of TERT is elevated in mildly active UC.
- Report cytoplasmic expression of hTERT in neutrophils: an immunoelectron microscopy study.
- The susceptibility region contains two genes, TERT and CLPTM1L, suggesting that one or both may have a role in lung cancer etiology.
- CSF1R may be a critical factor facilitating hTERT immortalization of epithelial cells.
- Transcriptional activation of hTERT by E6 oncoprotein is required for HPV-16/18-infected lung tumorigenesis.
- quantification of hTERT mRNA in plasma may be used as a marker for detection and monitoring of neoplastic colorectal disease.
- Alternative lengthening of telomeres (ALT) specific telomeric DNAs are produced by telomere metabolism specific to ALT, namely, homologous recombination and the rolling-circle replication mechanism.
- Regulation of the hTERT promoter activity by MSH2, the hnRNPs K and D, and GRHL2 in human oral squamous cell carcinoma cells.
- a contributory role for telomerase in tumourigenesis with activation occurring from neoplastic transformation and increasing with tumour progression
- Short and dysfunctional telomeres limit normal stem cell proliferation and predispose for leukemia by selection of stem cells with defective DNA damage responses that are prone to genome instability.
- Sequence variants at the TERT-CLPTM1L locus associate with many cancer types.