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Validated All-in-One™ qPCR Primer for TCF3(NM_003200.4) Search again
Product ID:
HQP017957
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AGM8, AGM8A, AGM8B, E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, p75
Gene Description:
transcription factor 3
Target Gene Accession:
NM_003200.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Functional collaboration between E47 and early B cell factor enables direct targeting of the surrogate light chain VpreB promoter for trans-activation during B cell development.
- E2A, along with histone acetyltransferases, regulates B cell development
- Evidence pertaining to leukemogenesis by the well-characterized E2A-fusion proteins E2A-PBX1 and E2A-HLF is reviewed and mechanistic implications are considered.
- E2a-Pbx1 and Bmi-1 are likely to play a role in the pathogenesis of human lymphoid leukemias through downregulation of the INK4A-ARF gene
- basic helix-loop-helix (bHLH) transcription factors, the E2A proteins (E47, E12 and E2/5), activated the human SHP promoter
- E2A-HLF induces annexin II by substituting for cytokines that activate downstream pathways of Ras
- E47 is involved in the regulation of p16(INK4a) transcription in cellular senescence
- in B cells, E47 and PU.1/IRF-4 interact with the E-box motifs and the EICE, respectively, and act synergistically in the activation of CIITA-PIII
- Ca2+ signaling can inhibit the transcriptional activities of E12 and E47 through direct binding of Ca2+/calmodulin to the basic sequence of E-proteins
- E2A is a positive regulator for one set of genes and a negative regulator for another set of genes in developing B lymphocytes
- study identifes E proteins(HEB, E2A) as AML1-ETO targets whose dysregulation may be important for t(8;21) leukemogenesis, as well as an E protein silencing mechanism that is distinct from that associated with differentiation-inhibitory proteins
- dHAND/E-protein (E2A, ME2, and ALF1) heterodimers have distinct DNA binding specificities
- E2A-PBX1 interacts directly with the KIX domain of CBP/p300 in the induction of proliferation in primary hematopoietic cells
- FHL2 interacts with Hand1 via the bHLH domain and is able to repress Hand1/E12 heterodimer-induced transcription
- A reporter assay with the p21 promoter demonstrated that Snail inhibited expression of p21 induced by E2A.
- Results identify a mouse bHLH-type factor (Tcfe2a, the human ortholog is TCF3), designated VDIR, as a direct sequence-specific activator of negative vitamin D response element (1alphanVDRE) in the human 1alpha(OH)ase gene promoter.
- E12 and E47 modulate both MyoD and Id1 degradation and may have implications for the physiological regulation of muscle
- the SREBP-1c.BETA2.E47 complex is in a DNA looping structure which is required for efficient recruitment of CREB-binding protein/p300
- The E2A-HLF-mediated over-expression of ABCB1 may play a role in the clinical phenotype of ALLs with a t(17;19), suggesting pharmacologic modulation of ABCB1 activity as a rational therapeutic strategy for this chemotherapy resistant subtype of ALL.
- The patients with immunophenotype of Pre-B-acute lymphoblastic leukemia were found to carry: E2A/PBX1 and E2A/HLF.
- Notch1 is an important "second hit" for the transformation of E2A deficient T cell lymphomas.
- activin A enhances PAX4 expression by enhanced transactivation of E47/E12 proteins and might result in a cumulative transactivation of the promoter
- Global gene expression analysis in neuroblastoma cells engineered to acutely express the E protein E47 and Id2, showed that p57Kip2 is a target of E47.
- Targeted-E2A-PBX1 inhibition leads to reduced expression of the EB-1 and Wnt16b genes; aberrant expression of these genes may be a key step in leukemogenesis in t(1;19)-positive pre-B leukemia.
- E47 coordinately regulates the expression of genes involved in cell survival, cell cycle progression, lipid metabolism, stress response, and lymphoid maturation.
- role of the E2A gene products in the progression of CsA-induced Epithelial-mesenchymal transition and provide novel insights into CsA-induced renal fibrosis.
- In childhood acute lymphoblastic leukemia and hypercalcemia, translocation was observed in E2A-HLF fusion protein.
- E2A proteins prevent lymphoma cell expansion, at least in part through regulation of Gfi1b and modulation of Gata3 expression.
- Involvement of the TCF3 gene in 19p13 rearrangements and in identifying novel and cryptic TCF3 translocations and also acts as a tumor suppressor gene in b-cell precursor acute lymphoblastic leukemia.
- results obtained with activated CD19(+) B cells show that the expression of transcription factor E2A, activation-induced cytidine deaminase, and Iggamma1 circle transcripts progressively decrease with age
- The prevalence of the E2A-PBX1 fusion gene is one of the highest that has been described thus far in childhood acute lymphoblastic leukemia.
- E2A and Na/K-ATPase beta1 subunit expression in epithelial cells are regulated by interactions between these proteins.