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Validated All-in-One™ qPCR Primer for TACR1(NM_001058.3) Search again
Product ID:
HQP017867
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
NK1R, NKIR, SPR, TAC1R
Gene Description:
tachykinin receptor 1
Target Gene Accession:
NM_001058.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P. [provided by RefSeq].
Gene References into function
- Demonstration of authentic and functional NK-1 receptors in microglia identifies substance P as a potentially important contributor to CNS inflammatory responses. during disease states.
- Tachykinin NK1 receptor-mediated relaxations are non-neurogenic (i.e., unaffected by tetrodotoxin) in penile tissues and are additive with neurogenogenic relaxations elicited by electric field stimulation.
- Involvement of the second extracellular loop (E2) of the neurokinin-1 receptor in the binding of substance P
- Human substance P receptor undergoes agonist-dependent phosphorylation by G protein-coupled receptor kinase 5 in vitro.
- role in human colon circular muscle; alterations in diverticular disease
- Analyses of the 5' flanking sequence in BM stroma and three neural cell lines indicated that sequence +1/+358 relative to the transcription start (TS) site could account for the differences in NK-1 expression between bone marrow and neurons.
- Subjects with depression showed decreased binding to NK-1 receptors across all cortical layers. The pathophysiology of depression, and the reported therapeutic benefit of NK-1 receptor antagonists, may involve NK-1 receptors in prefrontal cortex.
- This protein is expressed in the human Caco-2 cell line.
- Cgamma H2 of Met174 side chain is the site of covalent attachment to neurokinin-1 receptor of a substance P analog photoactivable in position 5.
- Substance P is expressed in human articular cartilage and is involved in chondrocyte mechanotransduction via the NK1 receptor in an autocrine and paracrine manner.
- The NK-1 receptor expressed in NT2-N neuronal cells is functionally involved in the regulation of macrophage inflammatory protein 1 beta and may play a major role in modulating neuronal functions related to immune regulatory activities within the CNS.
- NK-1R is regulated by NF-kappa B in human macrophages
- a role for substance P and its receptor, neurokinin NK(1) receptor, in the brainstem nuclei in the development of emesis (review)
- results provide the anatomical evidence that the NK1 receptors have a strong association with neuronal systems relevant to mood regulation and stress in the human brain, but do not suggest a region-specific role of the two isoforms in the CNS
- Data demonstrate the existence of two independent binding components in CHO cells transfected with the human neurokinin-1 (NK-1) receptor.
- Upregulated neurokinin-1 receptor expression in patients with sarcoidosis may potentiate substance P-induced proinflammatory cytokine production in patients with sarcoidosis
- demonstrate the presence of the tachykinin receptors NK1 and NK3 in platelets and present evidence for the involvement of NK1 in SP-mediated platelet aggregation
- reorganization of the plasma membrane has an effect on the activation of the raft-associated NK1R and the down-stream events such as recruitment of protein kinases
- NK-1 receptor immunoreaction could be detected in inner parts of the walls of large blood vessels and in the walls of small blood vessels of normal and chronic painful Achilles and patellar tendons
- NK-1 deficient cells did not proliferate when they were placed as cocultures with bone marrow stroma, which suggests that NK-1 signaling is important for the survival of NB cells in bone marrow.
- These data suggest substance P-induced vasodilatation delivered via microdialysis contains an NO component but does not contain an H1 receptor activation component at the doses tested.
- These results suggest that bFGF, TGF-beta1, and TNF-alpha in synovial tissue and fluid play a role in the regulation of long neurokinin-1 expression in synovial fibroblasts of rheumatoid arthritis patients.
- present data emphasize the role of NK1r in tachykinin-mediated neuronal processes regulating intestinal motility
- two binding sites are independent and non-interconvertible on the time scale of these experiments
- ERK1/2 is activated by a chimeric neurokinin 1 receptor-beta-arrestin1 fusion protein
- Results demonstrate that there are unique characteristics of neurokinin-1 receptor (NK-1R) expression and NK-1R-mediated signaling between undifferentiated THP-1 cells and THP-1 cells differentiated to the macrophage phenotype.
- this study has demonstrated that the NK(1) receptor is widely distributed in the amygdala, and has shown for the first time a high relative density of NK(1) receptor-immunoreactive cell bodies in the basal nucleus of Meynert.
- Data explored a role for NK1 as a potential target for breast cancer treatment and suggest that similar mechanisms might be operative for other cancers showing preference for bone marrow metastases.
- NK(1) receptors contribute to active vasodilatation, and combined NK(1) receptor desensitization and NO synthase inhibition further diminishes active vasodilatation.
- NK1 receptors modulate CaV2.3 using three different signaling mechanisms: a fast inhibition mediated by Gbeta gamma, a slow inhibition mediated by Galpha(q/11), and a slow stimulation mediated by protein kinases C.
- Nonneuronal substance P and its receptor NK-1 might have a role in psoriasis, also during chronic stress.
- studied gene variants of Tachykinin Receptor 1 in 167 suicide attempters (affective spectrum n = 107, schizophrenia spectrum n = 35, borderline personality disorder n = 25), 92 individuals who committed suicide and 312 healthy subjects
- This study showed that the same isoforms of the neurokinin-1 receptor are present in human retinoblastoma cell lines WERI-Rb-1 and Y-79. Both L-732,138 and L-733,060 can induce apoptosis in these cell lines
- Functional consequences of alteration of N-linked glycosylation sites on NK1R were studied.
- Changes in SP receptor expression during chronic inflammation suggest that SP receptors are potential targets for therapeutic regulation of the inflammatory bowel disease.
- NK1 receptor mRNA and protein expression were enhanced in obstructive sleep apnea.
- Evidence was also obtained that the proper clustering of the receptor in these microdomains was important for effective agonist-induced NK1-R signaling and for its interaction with beta-arrestin2.
- NK1R expressing neurons in different subregions of the ventral respiratory group have diverse roles and provide insight on the modulatory role of substance P.
- T-2328 is a potent, centrally active NK(1) antagonist.
- all three subtypes of NKRs are expressed in native human airway smooth muscle cells & IP3 levels are the primary mediators of NKR-stimulated initial [Ca2+]i increases, whereas store-operated Ca2+ channels mediate sustained phase of the [Ca2+]i increase.
- NK-1R and GRP-R were also expressed at both protein and mRNA levels in cells associated with blood vessels and cells in the interstitial tissue in rheumatoid and osteoarthritis
- Presence of NK1R in tenocytes of the human Achilles tendon is reported.
- genes encoding corticotropin releasing hormone receptor 1, tachykinin receptor 1, gastrin releasing peptide, and gastrin releasing peptide receptor were selected as candidates for PD based on their biology
- full-length form of NK1R may have important significance in cognitive functions and deficiency of this isoform may be relevant in neurologic and psychiatric manifestations of neuroAIDS.
- differential signaling properties attributable to the carboxyl terminus of NK1R
- In healthy human colon a higher expression level of the TACR2 mRNA alpha isoform, the gene encoding the functional tachykinin NK(2) receptor, was observed in comparison to TACR1 and TACR3 mRNAs genes encoding for NK(1) and NK(3) receptors respectively