|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for BSG(NM_001728.3) Search again
Product ID:
HQP017820
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, TCSF
Gene Description:
basigin (Ok blood group)
Target Gene Accession:
NM_001728.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily.
Gene References into function
- cyclophilin B receptor; mediates calcium signaling and neutrophil chemotaxis induced by cyclophilin B
- Active site residues of cyclophilin A are crucial for its signaling activity via CD147
- Basigin (CD147) is expressed on melanoma cells and induces tumor cell invasion by stimulating production of matrix metalloproteinases by fibroblasts
- Extracellular matrix metalloproteinase inducer is induced upon monocyte differentiation and is expressed in human atheroma as well as in GM-CSF-differentiated peripheral blood monocytes and macrophage foam cells.
- EMMPRIN stimulates fibroblast MMP2 release.
- expressed in Hodgkin's lymphoma and anaplastic large cell lymphoma
- Chlamydia pneumoniae infection implicated as an important etiologic factor of atherosclerosis was found to be associated with the induction of matrix metalloproteinases (MMPs), upregulated by EMMPRIN.
- role of EMMPRIN in tumor invasion, metastasis, and neoangiogenesis by stimulating extracellular matrix remodeling around tumor cell clusters, stroma, and blood vessels
- expression of EMMPRIN is responsible for the increased activity of matrix metalloproteinases in MDR cell lines
- CD147 is a transmembrane protein involved in reproduction, neural function, inflammation and tumor invasion [review]
- EMMPRIN may be an important regulatory factor involved in the biological behaviors of giant cell tumor
- Antibody targeting of T cell activation-associated antigen CD147 prevents T cell receptor (TCR) stimulation-dependent reorganization and clustering of membrane microdomains and suggests a general mechanism for inhibition of receptor signaling.
- study indicates the functional interaction between CD98 and CD147 in the regulation of cell fusion
- Expressed in human endometrium during menstrual cycle. Expression and glycosylation augmented by progesterone. May be involved in extracellular matrix(ECM) breakdown at interface between endometrial cells and ECM by using EMMPRIN-bound MMP-1.
- showed highly specific association with caveolin-1 on the surface of multiple cell types
- Our results suggest an importance of the direct cell-cell interaction involving EMMPRIN rather than humoral factors such as cytokines for pro-MMP-2 production and activation followed by tumor progression, invasion, and metastasis in laryngeal cancer
- extracellular matrix degradation by fibroblasts is controlled through the microvesicular release of EMMPRIN from tumor cells
- model in which tumor cell-associated EMMPRIN stimulates MMPs, as well as EMMPRIN expression in tumor stroma
- EMMPRIN has a role in progression of human breast cancer
- placenta and fetal membranes express EMMPRIN, with the potential to stimulate MMP production, facilitating fetal membrane rupture and detachment of the placenta and fetal membranes from the maternal uterus at the time of parturition.
- CD147 is a major carrier of beta1,6-branched polylactosamine sugars on tumor cells
- Up-regulation of EMMPRIN by epidermal growth factor and transforming growth factor-beta, demonstrate a role in wound healing.
- a cyclophilin-related protein is involved in CD147 cell surface expression
- review of role of emmprin as a major mediator of malignant cell behavior
- Expression of EMMPRIN protein may be detected in hepatocellular carcinoma(HCC), but it may play little role in the invasion and metastasis of HCC.
- ECM remodeling is under the control of induction and inhibition of matrix degrading protease and the novel MMP inducer, EMMPRIN may play a role in influx and differentiation of monocytes and destabilizing atheroma
- fibroblast-derived MMPs but not those from tumor cells are important for in vitro collagenolysis and that this process is promoted by tumor cell-expressed EMMPRIN
- Results suggest a novel tumor angiogenesis mechanism in which tumor-associated EMMPRIN functionally mediates tumor-stroma interactions and directly contributes to tumor angiogenesis and growth by stimulating VEGF and MMP expression.
- Depletion of CD147 by RNA interference was found to increase the production of amyloid beta-peptides.
- expression of CD147 is upregulated during the differentiation of monocyte THP-1 cells to macrophage cells, and CD147 induces the secretion and activation of MMP-2 and MMP-9 and enhances the invasive ability of THP-1 cells
- cyclophilin 60 regulates cell surface expression of CD147/EMMPRIN
- Increased CD147 on monocytes/macrophages in rheumtaoid arthritis may cause elevated matrix metalloproteinase secretion, cell invasion & cyclophilin-A-mediated cell migration into the joints; this may lead to to cartilage & bone destruction.
- These results suggest that EMMPRIN overexpression occurs at a very early stage of oral carcinogenesis and plays a contributing role in OSCC tumorigenesis.
- variations in EMMPRIN glycosylation forms are associated with either MMP-2 or MMP-9 activity
- The colocalization of EMMPRIN, MT1-MMP and TIMP-2 in human cervical carcinomas seems to be involved in a specific distribution pattern of tumor cell bound MMP-2, which is related with local proteolytic activity.
- results indicated that expression of EMMPRIN protects cancer cells from anoikis and that this effect is mediated at least in part by a MAP kinase-dependent reduction of Bim
- EMMPRIN and MT1-MMP are upregulated on monocytes in acute MI. During cellular interactions, EMMPRIN stimulates MMP-9 in monocytes and MMP-2 in smooth muscle cells.
- CD147 may be important factor in progressive joint destruction of rheumatoid arthritis(RA) and may be potential therapeutic target in RA treatment.
- CD147 downregulation by RNAi technology decreases the invasive capability of prostate cancer cells.
- findings show for the first time that EMMPRIN is overexpressed in malignant ovary tumors
- breast cancer cells expressed EMMPRIN isoforms differing in the presence or absence of Lewis X glycan structures
- Increased expression of EMMPRIN in tumor cells is associated with poor prognosis of patients with papillary renal cell carcinoma
- results show that in both tumor and fibroblast cells EMMPRIN regulates VEGF production via the PI3K-Akt pathway but not via the MAPK, JUN, or p38 kinase pathways
- Data demonstrated that there are two different forms of HAb18G/CD147 differing in degree and types of glycosylation.
- These data show that elevated cytokines may play a role in the establishment of ectopic endometrium in the peritoneal cavity without any change in EMMPRIN expression, in fact the inhibitory effect of TGF-beta1 involved a reduction in EMMPRIN mRNA levels.
- MT1-MMP-dependent cleavage eliminates the functional N-terminal domain of EMMPRIN from the cell surface, which is expected to down-regulate its function.
- Upregulated EMMPRIN contributes to growth and angiogenesis of gastric carcinoma
- We propose that TM3 of MCT1 lies alongside the TM of basigin with Arg86 adjacent to Glu218 of basigin.
- CD147 siRNA down-regulated the expression of vascular endothelial growth factor in malignant melanoma cells and reduced the migration of vascular endothelial cells.
- Increased EMMPRIN levels in gingival crevicular fluid are associated with the enhanced severity of periodontal inflammation, indicating that it can participate in the regulation of disease progression.
- shrew-1 has a function in the regulation of cellular invasion, which may involve its interaction with CD147
- CD147 may assume a dual role, since it had intrinsic stimulative effects on breast cabcer tumor invasion in vitro as well as increasing resistance to P-gp substrate drugs.
- BSG residues (22)AAGTVFTTVEDLGSKILLTCSLNDSATEV(50) may play a critical role in the inhibitory functions of monoclonal antibody HAb18G/CD147 on matrix metalloproteinase secretion and tumor invasion.
- Vesicles shed by ovarian cancer cells may induce proangiogenic activities of human umbilical vein endothelial cells (HUVECs) by a CD147-mediated mechanism.
- HAb18G/CD147 plays an important role in HCC invasion and metastasis mainly via modulating fibroblasts
- degree of staining of CD147 was significantly higher in hepatocellular (HCC) tumor tissues than non-tumor tissues; expression was significantly elevated in HCC tissue specimens from patients with a low value of serum AST and gamma-GTP
- Multidrug resistant breast cancer cells with p-glycoprotein substrates could affect outcome through modulating the production of MMP9.
- Low EMPRINN expression was found more often in serous tumors than in other types of epithelial ovarian cancer. It was associated with a better prognosis.
- the effect of Extracellular matrix metalloproteinase inducer (EMMPRIN)on its expression in corneal and skin fibroblasts
- triggering CD43 and the underlying signaling pathways enhance LFA-1 adhesiveness while CD43 also negatively regulates LFA-1 induction via other receptors by dynamic interaction with either LFA-1 or CD147.
- EMMPRIN and MMP-2 are the major determinants of malignancy in cancers.
- Coincubation of platelets with monocytes induced EMMPRIN-mediated nuclear factor kappaB activation in monocytes with increased MMP-9, interleukin-6, and tumor necrosis factor-alpha secretion by monocytes
- CD147, via the selective inhibition of specific downstream elements of the Vav1/Rac1 route, contributes to the negative regulation of T-cell responses.
- EMMPRIN was preferentially expressed in most NSCLCs. High levels of expression were associated with early T stage and well-differentiated adenocarcinoma
- The expression of EMMPRIN confers resistance to radiotherapy. Therefore, EMMPRIN expression in cervical cancer may be regarded both as a prognostic factor and a therapeutic target.
- Data propose that the CD147-NOD2 interaction serves as a molecular guide to regulate NOD2 function at sites of pathogen invasion.
- EMMPRIN may regulate MMPs and be involved in prostate cancer progression
- the crystal structure of HAb18G/CD147 provides a good structural explanation for the established multifunction of CD147 mediated by homo/hetero-oligomerizations
- soluble EMMPRIN probably triggers the promotion of cancer invasion in vivo
- cell surface basigin functions as a membrane receptor for soluble basigin and this homophilic interaction is not dependent upon glycosylation of the basigin ligand
- CD147 expression influences Abeta levels by an indirect mechanism involving MMPs that can degrade extracellular Abeta.
- Investigations as to whether specific tumor-stromal cell interactions mediated by CD147 promote colon cancer growth by utilizing small interfering (si)RNAs directed against human CD147.
- The overall EMMPRIN expression in the epithelial lining of the 3 different types of odontogenic cyst was significantly higher than in the dental follicles.
- The findings point to a role of EMMPRIN for the progression of adenocarcinoma of the lung that is unrelated to its function as inducer of MMPs.
- Results indicate that CD147 is involved in T-lymphoma progression.
- Increased CD147 expression is associated with differentiated thyroid carcinoma.
- We are characterizing the functional importance of EMMPRIN in bladder cancer in order to evaluate this protein as a new target molecule for therapy.
- Cardiac-restricted overexpression of EMMPRIN causes myocardial remodeling and dysfunction in aging mice.
- ubiquitination of P-glycoprotein and CD147 might be a novel method for tumor therapy
- Results suggest that HAb18G/CD147 enhances the invasion and metastatic potentials of human hepatoma cells via integrin alpha3beta1-mediated FAK-paxillin and FAKPI3K-Ca(2+) signal pathways.
- EMMPRIN regulates migration, MMP production by mouth cancer SCC cells and deposition of the TN-C matrix.
- CD147 interacts with MCT1 and 4 to promote tumor cell glycolysis, resulting in the progression of MM
- Proteome analysis of multidrug resistance of oral squamous carcinoma cells using CD147 silencing is reported.
- Downregulation of CD147 affects hepatocellular carcinoma cell structure and function.
- Significantly higher CD147 expression is associated with prostate cancer.
- Tumor growth positively correlated and animal survival negatively correlated with increasing EMMPRIN expression. FaDu/E tumor growth was significantly larger at 4 weeks compared with FaDu tumors (P = .006).
- CD147 may functionally mediate tumor cells invasion and neoplasm invasiveness in multidrug resistnt hepatocellular carcinoma.
- Interactions among hyaluronan, CD44, and emmprin contribute to regulation of monocarboxylate transporter in the cell membrane of breast cancer cells.