|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for TRIM21(NM_003141.3) Search again
Product ID:
HQP017729
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
RNF81, RO52, Ro/SSA, SSA, SSA1
Gene Description:
tripartite motif containing 21
Target Gene Accession:
NM_003141.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules.
Gene References into function
- that Ro52 may be downregulated by the ubiquitin-proteasome pathway in vivo
- First evidence is provided for the involvement of SS-A in CD28-induced production of IL-2, which is critical in the pathophysiology of autoimmune diseases such as Sjogren's syndrome and systemic lupus erythematosus.
- Immunologic analysis of the stable protein regions with sera from patients with Sjogren's syndrome shows that immunodominant epitopes to a large extent are localized in the structurally stable parts of Ro52
- Reactivity to p200 is a dominant but not uniform anti-Ro 52 response in women whose children have congenital heart block
- Ro52 is a RING-finger-type E3 ubiquitin ligase.
- These results suggest that UnpEL colocalizes with the unubiquitinated form of Ro52 to the cytoplasmic rod-like structures.
- Antibody subsets may represent important biomarkers to predict a complication in pregnant lupus women with Ro52 antibodies.
- analysis of subcellular location and function of Ro52beta
- Ro52 and UnpEL transregulate each other by ubiquitination and deubiquitination
- Presence of serum tripartite motif-containing 21 antibodies in patients with esophageal squamous cell carcinoma
- Increased expression of Ro52 in patients may be directly involved in the reduced cellular proliferation and increased apoptotic cell death observed in Sjogren's syndrome and systemic lupus erythematosus.
- heterologous RING, B-box 2, and CC domains from related TRIM proteins can functionally substitute for TRIM5alpha(rh) domains.
- These data suggest a key role for Ro52 RING finger protein in the regulation of p27 degradation and S-phase progression in mammalian cells.
- Nuclear injury and the translocation of SSA/Ro and SSB/La antigens to the fetal cardiocyte plasma membrane were common downstream events of Fas and TNF receptor ligation.
- The data suggest that the normal function of TRIM21 involves regulation of IgG functions and that TRIM/B30.2 molecules may have broader and unsuspected roles in innate immunity, beyond that of retroviral restriction.
- A TRIM21 antibody bipolar bridging mechanism may contribute to the pathogenic accumulation of anti-TRIM21 autoantibody immune complex in autoimmune disease
- Increased Ro/SSA 60 and La/SSB mRNA expression in minor salivary glands in primary Sjogren's syndrome (pSS) suggest that these 2 autoantigens, but not Ro/SSA 52, are involved in tissue-specific autoimmune response in pSS.
- Plays a role in mediating the anti-proliferative or pro-apoptotic effects of autoimmune-related cytokine interferon-alpha.
- results obtained from Ro52 are extendable to the entire TRIM protein family
- These data support the novel notion of the association between Ro52 with hDCP2 protein in cytoplasmic p-bodies, playing a role in mRNA metabolism in response to cellular stimulation.
- TRIM21 is a previously undescribed type of IgG receptor based on a non-Ig scaffold whose interaction at the fundamental level-structural, thermodynamic, and kinetic-is evolutionarily conserved.
- demonstrates a novel role for Ro52 in turning off and thus limiting IRF3-dependent type I IFN production by targeting the transcription factor for polyubiquitination and subsequent proteasomal degradation
- Ro52 has both cytoplasmic and nuclear substrates, and mediates ubiquitination through UBE2D1 in the cytoplasm and through UBE2E1 in the nucleus.