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Validated All-in-One™ qPCR Primer for SP1(NM_138473.2) Search again
Product ID:
HQP017640
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
-
Gene Description:
Sp1 transcription factor
Target Gene Accession:
NM_138473.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- The Sp1 transcription factor interacts with the human eNOS promoter at a region which includes a putative Sp1 site (-104 to -95). This interaction activates eNOS transcription.
- role in interferon-gamma mediated suppression of macrophage lipoprotein lipase gene transcription
- role in identifying sterol-indendent regulatory elements in human ATP-binding cassette transporter A1 promoter
- hinge region of the human papillomavirus type 8 E2 protein activates the human p21(WAF1/CIP1) promoter via interaction with Sp1
- role in regulating growth-promoting gene IEX-1
- role in regulating 11beta-hydroxysteroid dehydrogenase type 2 promoter
- AP-2 down-regulates transcription of the human SOD2 gene via its interaction with Sp1 within the promoter region.
- The expression of the ABO promoter is influenced by the binding of transcription factor Sp1 or Sp1-like protein(s) in both erythroid and epithelial cell lineages.
- Role of Sp1 and Sp3 in the nutrient-regulated expression of the human asparagine synthetase gene
- Binding of Sp1 to the 5' end Sp1-I site is critical for maximal activity of the Fc gamma RIIIA promoter Pmed1, which directs NK/T cell-specific expression of heterologous genes.
- Enhancement of binding between the c-Jun-Sp1 complex and the Sp1 oligonucleotide was observed in cells treated with PMA, suggesting the possible interaction of c-Jun-Sp1 with GC-rich binding sites in the 12(S)-lipoxygenase gene promoter.
- Ets-1 and Sp1 have a role in regulating FasL expression in human vascular smooth muscle cells
- huntingtin interacts with Sp1 and TAFII130; transcriptional activity of SP1 and TAFII130 disrupted in early Huntingtin's Disease
- Sp1 and the ets-related transcription factor complex GABP alpha/beta functionally cooperate to activate the utrophin promoter.
- recruits SV40 capsid proteins to the viral packaging signal, ses
- phosphorylated during late Herpes simplex virus type 1 infection and may contribute to decrease of IE and E gene expression
- demonstrates role of the Sp1 protein in basal and estrogen-induced growth and gene expression in breast cancer
- conclude that E2F proteins and Sp1 play an important role in the control of p18 expression
- Mutation of the progesterone receptor promoter Sp1 site eliminated the effect of hPR activation. Activity was increased by Sp1 overexpression. Dephosphorylated Sp1, abundant in decidual cells, enhances binding to DNA & hPR increasing promoter activity.
- The hexosamine pathway regulates the plasminogen activator inhibitor-1 gene promoter and Sp1 transcriptional activation through protein kinase C-betaI and -delta.
- cooperation with hepatocyte nuclear factor-4 leads to transcriptional activation of the human haem oxygenase-1 gene promoter in a hepatoma cell line
- RAI acts as an efficient inhibitor of HIV-1 gene expression in which both NF-kappaB and Sp1 play major roles.
- role in activating p21(WAF/CIP1) expression accompanying 3,3'-diindolylmethane induced G(1) cell cycle arrest in human breast cancer cells
- Results suggest that Sp1 and Sp3 associate with the hTERT promoter, recruiting HDAC for the localized deacetylation of nucleosomal histones and transcriptional silencing of the hTERT gene in normal human somatic cells
- Sp1 plays a role in regulation of promoter activity and in PKA-mediated expression of mitochondrial serine:pyruvate aminotransferase
- TGF-beta1 inhibition of COL2A1 gene transcription in articular chondrocytes is mediated by an increase of the Sp3/Sp1 ratio and by the repression of Sp1 transactivating effects on that gene
- IGFBP-3 promoter is activated by Trichostatin A-upregulated Sp1
- Sp1 regulates human reduced folate carrier gene expression
- Sp1 regulates basal endoglin transcription in human and mammalian cells
- role of transcription factors Sp1 and Sp3 in the regulation of telomerase activity and human telomerase reverse transcriptase (hTERT) in Jurkat T cells
- there is a mechanically coupled transcriptional circuit that promotes binding of p38 to Sp1 in the nucleus
- findings underline an essential role of AP-2/Sp1 recognition sites in UVB-mediated VEGF expression by the keratinocyte-derived cell line HaCaT
- role in regulating megakaryocyte-specific glycoprotein VI promoter
- This protein plays a role in the identification of regulatory elements in the human adipose most abundant gene transcript-1(apM-1)promoter.
- Sp1 binds to specific elements of the proximal promoter and interacts with factors bound to distal enhancer elements to govern the hepatic activity of the p21 promoter under basal or inducible conditions.
- Binding of Sp1 to the proximal promoter links constitutive expression of the human uPA Phosphorylation of Sp1 determines the presence in vivo and the functionality of the GC-/GA-rich element of the uPA regulatory region in PC3 cells
- TCL1 oncogene transactivation by Sp1.
- confirms the synergistic transactivating role of NF-YA isoforms and Sp1 for the cystathionine beta synthase 1b promoter
- role in enhancing cyclin D1 promoter activity in conjunction with galectin-3
- Tat and trans-activation-responsive (TAR) RNA-independent induction of HIV-1 long terminal repeat by human and murine cyclin T1 requires this protein.
- role for Sp1 in the regulation of expression of the angiogenic factor TP in colon cancer WiDr cells
- Alterations in the binding activity of Sp1 transcription factor to the sodium/iodide symporter (NIS) promoter results at least in part in reduced expression and transport of NIS in thyroid tumors.
- GA-binding protein (GABP) and Sp1 and retinoid receptors are required to mediate retinoic acid responsiveness of CD18 transcriptional activation in myeloid cells.
- role in regulating vascular endothelial growth factor-A gene transcription by activating two proximal GC-rich promoter elements
- Functional analysis of a hypersensitive site in the 5' flanking region of the hEPCR gene in endothelial cells identified multiple regions, containing high and low homology consensus Sp1 binding sequences, that were protected from methylation.
- results suggest that oxidized phospholipids inhibit transcription of the thrombomodulin gene in vascular endothelium by inhibiting the binding of retinoic acid receptor beta-retinoid x receptor alpha heterodimer and Sp1 and Sp3 to thrombomodulin promoter
- Expression of the hypoxia marker carbonic anhydrase IX is critically dependent on SP1 activity.
- Sp1 has a role in valproic acid-induced expression of Galphai2
- Regulates leukotreiene C4 synthase gene expression in mononuclear phagocytes.
- HMGI-Y physically interacts with Sp1 and C/EBP beta and facilitates the binding of both factors to the insulin receptor promoter
- Specific protein-1 is a universal regulator of UDP-glucose dehydrogenase expression: its positive involvement in transforming growth factor-beta signaling and inhibition in hypoxia
- This protein and Sp3 are involved in up-regulation of human deoxyribonuclease II transcription during differentiation of HL-60 cells.
- Sp1 and Sp3 regulate human CETP promoter activity through three Sp1/Sp3 binding sites in a distinct manner
- IRF1 represses CDK2 gene expression by interfering with SP1-dependent transcriptional activation.
- SP1 and ETS have a role in regulation of caspase 8
- Sp1 is found to bind strongly to both distal and proximal GC-rich elements as a transcriptional modulator of DNA topoisomerase II alpha promoter activity, with activator or repressor functions depending on protein/DNA context.
- PTEN expression induced decreased SP1 DNA binding by dephosphorylating SP1.
- The results indicate that Sp1 is the protein mediating the basal transcription of MGST1. It appears that both the Sp1 and Sp3 proteins are important for the basal expression of PGES
- Sp3 and Sp1 have roles in regulating monoamine oxidase B in human tumor cells
- the constitutive overexpression of CTGF in SSc fibroblasts seems to be independent of TGFbeta signaling but dependent at least in part on Sp1
- HNF4-alpha, HNF3-beta and Sp1/Sp3 are important in regulation of prothrombin expression
- results indicated a predominant role for a single Sp1 binding site in regulating human cartilage-gp39 promoter activity in macrophages
- transcription factors Sp1 and c-Jun have roles in regulation of keratin 16 gene expression by epidermal growth factor
- SP1 activates transcription of the LHR gene at two SP1 sites. Its mutation reduced the inhibition by EAR2 and abolished the inhibition by EAR3.
- Our data suggest that thrombin/PAR-1 may inhibit Sp1-dependent HCMV replication, which might be an important regulatory mechanism for HCMV persistence and replication in RPE
- USF and Sp1 proteins interact and regulate human deoxycytidine kinase promoter activity
- results suggest that 2-DG, like low glucose or hypoxic condition, down-regulates Sp1 activity, but through hyper-GlcNAcylation instead of hypo-GlcNAcylation
- Sp1 is a bile acid-responsive transcription factor that mediates DR5/TRAIL-R2 gene expression downstream of JNK.
- data provide an alternative mechanism of LDL receptor gene expression by non-classical estradiol- and tamoxifen-stimulated induction through an ER-alpha/Sp1 complex
- Biotin affects gene expression in Jurkaat cells.
- E2F and Sp1/Sp3 synergize but are not sufficient to activate the MYCN gene in neuroblastoma
- transcription factors HNF3, MAZ, and Sp1 are required for high-level expression of the protein S gene in hepatic cells, but in non-hepatic cells such as HeLa cells, an unknown factor(s) binds to the Sp1 region and disturbs the action of Sp1 and MAZ
- up-regulation of uPAR expression by Bcl-2 in hypoxia is modulated by Sp1 DNA binding activity through the ERK signaling pathway
- Sp1 has a critical and indispensable role in the regulation of cystathionine beta-synthase
- BACE1 gene expression is tightly regulated at the transcriptional level, and transcription factor Sp1 plays an important role in regulation of BACE1 to process APP generating Abeta in Alzheimer's disease.
- the cooperation of HIF-1 with Sp1/Sp3 confers transcriptional activation under hypoxia to RORalpha4
- Perifosine increases Sp1 binding and enhanced p21(waf1/cip1) transcription.
- SREBP-1c and Sp1 interact to regulate transcription of the gene for phosphoenolpyruvate carboxykinase (GTP) in the liver
- S-nitrosoglutathione increases Sp1 binding to DNA and transcription of CFTR at physiological concentrations.This increase in Sp1 binding probably occurs through a post-translational cysteine S-nitrosylation.
- Sp1 has a role in upreglation of VEGF by EWS-ETS oncoproteins in Ewing's sarcoma
- a novel, functional binding element in the proximal region of the TN-C promoter mediating responsiveness to TGF-beta involving Smad3/4, Sp1, Ets1, and CBP/p300
- Transcription factor SP1 has no effect on Monoamine oxidase B gene expression.
- transcription factor Sp1 is involved in the p38MAPK-mediated control of p21WAF1 regulation on vascular smooth muscle cells
- Suppression of Sp1 expression by small interference RNA reduced the stimulation of p27Kip1 promoter activity by vitamin D3 in prostatic cancer cells.
- Sp1 was associated with the +571 estrogen response element/Sp1 site in the endogenous progesterone receptor gene in the absence and in the presence of estrogen
- p21(Waf1) can activate the transcription of p16(INK4); this effect is GC-box dependent; and the transcription factor Sp1 plays a key role in this event
- An SP1-binding element determines the function of the TATA boxless CD1D-proximal promoter.
- PPARgamma1 has bifunctional properties in the regulation of KDR gene expression mediated via interaction with both Sp1 and Sp3
- the association of hMI-ER1 with Sp1 represents a novel mechanism for the negative regulation of Sp1 target promoters
- human secretin gene is controlled by the Sp1/Sp3 transcription factors ratio
- co-transfection with antisense Sp1 oligonucleotide suggests that hypoxia induction of the RTP801 promoter is mediated by Sp1
- Sp1 has a role in regulating CDC42BPG expression
- Sp1 and VEGF may have roles in the progression of gastric neoplasms
- Sp1 and Sp3 proteins play a physiologically important role in positively and negatively regulating the 15-LOX2 gene expression, respectively.
- Interplay between Pur repressor isoforms and Sp1 and Smad coactivators may regulate alpha-actin enhancer output in TGFbeta1-activated myofibroblasts during episodes of wound repair and tissue remodeling.
- physical interaction of Vpr with Sp1 and p53 could modulate transcriptional activity of p21.
- TGF-beta1-induced c-met expression was contingent on a functional Sp1.
- Sp1 has a role in Akt-mediated induction of VEGF expression through an HIF-1-independent mechanism
- DIM-C-pPhCF3 induced p21 expression through a novel mechanism that involves PPARgamma interactions with both Sp1 and Sp4 proteins bound to the proximal GC-rich region of the p21 promoter.
- results suggest that the interaction of KSHV LANA with Sp1 up-regulates the telomerase promoter, potentially contributing to the immortalization of KSHV-infected cells
- Co-operative functional interactions between Sp1 & NF-Y are required to direct the activity of the ATP1A3 promoter predominantly in neuronal cells.
- the common genetic variants at the PCOL2 and Sp1 sites, and importantly, their interactive effects, may contribute to bone mineral density variation in elderly Caucasian females
- The reduced binding affinity of Sp1/Sp3 to the T allele explain earlier observations of a reduced t-PA release and an increased risk of myocardial infarction in individuals carrying this allele.
- Activation of ERK-MAPK pathway and Sp1 transcription factor play a pivotal role in the induction of TIMP-3 by TGF-beta in chondrocytes.
- results show that there are genuine Sp1/Sp3 or Egr-1 controlled genes showing no cross-regulation of Sp1/Sp3 and Egr-1 through the same DNA-binding site
- interplay between REST and Sp1 determines the cell-specific expression of REST target genes
- Sp1 and MZF1 are involved in the transcriptional regulation of the hST6GalNAc IV gene in Jurkat T cells
- activation by SREBP/Sp1 requires antagonism of KLF11 repression
- protein kinase Calpha transcriptional repression via Sp1 by wild type p53 is involved in inhibition of multidrug resistance 1 P-glycoprotein phosphorylation
- transforming growth factor beta type II receptor promoter activity and acetylation of Sp1 by recruitment of PCAF/p300 to a Sp1.NF-Y complex are induced by Trichostatin A
- induction of KSHV lytic cycle by butyrate is mediated through binding of response element in KSHV ORF50 promoter to Sp1.
- Electrophoretic mobility shift assay also showed a significantly higher binding efficiency of nuclear protein or pure Sp1 protein to the T allele compared with the G allele of the EGF receptor.
- Proximal promoter of GIP-R gene contains a GC-rich region capable of binding to Sp1 and SP3.
- consistently observed Sp1 and NFkappaB binding activity to the promoter of either gene during cell differentiation and the decrease associated with apoptosis upon long-term treatment with differentiation inducers
- HIV-1 Tat regulates the SOD2 basal promoter by altering Sp1/Sp3 binding activity
- The beta-hairpin region of Sp1 appears to function as a scaffold and has an important effect on the DNA binding properties of C2H2-type zinc finger peptides.
- In human epidermal keratinocytes, Sp1 is a differentiation-specific activator and a downstream target of E2F-mediated suppression of the differentiation-specific marker, transglutaminase type 1
- human OGG1 is regulated by cadmium through suppression of Sp1 activity
- PARP-1 expression appeared to be co-ordinated with that of Sp1 and Sp3 in primary cultured cells, suggesting that PARP-1 may play some important functions during the proliferative burst that characterizes wound healing.
- has an intimate part in transcriptional regulation of the lh receptor gene.
- Sp1 is a strong activator of dopamine transporter transcriptional activity.
- Data demonstrate that the constitutive activity of the human melanocortin-4 receptor promoter is dependent upon Sp1 and 3 transcription factors.
- both Sp1 binding sites could be important to complete JC virus replication cycle in absence of HIV-coinfection
- -250G>C mutation results in the loss of an SP1 binding site, and transient transfection assays demonstrated that such mutation strongly impairs promoter activity
- Sp1 binds to the proximal region of the interleukin (IL)-1alpha promoter and may play a role in regulation of IL-1alpha gene expression.
- study reports the first characterization of the hPEPT1 promoter and shows the significant role of Sp1 in the basal transcriptional regulation of hPEPT1
- role in regulating Elf-1 like factor in epithelial cells
- PPARgamma ligands inhibit Fn gene expression in NSCLC cells through PPARgamma-dependent and -independent pathways that affect both CREB and Sp1.
- Sp1 and Sp3 have roles in regulation of human telomerase reverse transcriptase promoter activity by the bioactive sphingolipid ceramide
- Sp1 and YY1 transactivate human ATP2C1 promoter via cis-enhancing elements and that incomplete upregulation of ATP2C1 transcription contributes to keratinocyte-specific pathogenesis of Hailey-Hailey disease.
- PGE2 increases VEGF transcriptionally and involves the Sp-1 binding site via a cAMP-dependent mechanism involving EP2 and EP4 receptors
- Our results suggest that MUC6 transcription is regulated by NFkappaB and Sp family members.
- Demonstration of the different intranuclear features of Sp1 and Sp3 provides further evidence that Sp3 is not a functional equivalent of Sp1.
- Sp1 is an inhibitor of beta-like globin gene transcription during erythroid terminal differentiation.
- Sp1 interacts with Hsp90alpha to recruit it to the promoter of 12(S)-lipoxygenase and then to regulate gene transcription by modulating the binding ability of Sp1 to promoters
- The Sp1 Transcription Factor might impact gastric cancer development and progression through regulating angiogenesis, a critical aspect of cancer biology.
- These results suggest that the upregulation of TN-C expression by PDGF involves Ets family transcription factors, co-operating with Sp1.
- In addition, the transcription factor Sp1 that is involved in the Ras/ERK-mediated control of p21WAF1 regulation in VSMC in response to PDGF has now been identified.
- AP-2alpha and Sp1 are strong transcriptional regulators of KiSS-1
- TCR-induced IL-21R expression is driven by TCR-mediated augmentation of Sp1 protein levels and may partly depend on the dephosphorylation of Sp1
- Negative androgen-responsive element in region of -277 to -262 and positive Sp1 element in region of +14 to +35, respectively.
- in the absence of IFN treatment, activation of PKR basal expression is mediated by Sp1 and Sp3 proteins in a cooperative manner
- The AP-1 site in the u-PAR promoter seems to be a less tumor-specific regulator than the Sp1 and AP-2 alpha.
- HER-2/neu induces the binding of Sp proteins and HDAC1 to the RECK promoter to inhibit RECK expression and to promote cell invasion
- Sp1 deposition in hyper-phosphorylated tau deposits may have functional consequences in the pathology of AD and other tauopathies.
- dioxin receptor is silenced by promoter hypermethylation in human acute lymphoblastic leukemia through inhibition of Sp1 binding
- the GC-rich part of the 5-lipoxygenase gene promoter includes a novel Sp1 site
- These findings suggest that mutant huntingtin dissociates Sp1 from target promoters, inhibiting transcription of specific genes.
- Our results demonstrate that regulation of histone modification by an HDAC inhibitor apicidin contributes to induction of cyclin E expression and this effect is Sp1-dependent.
- identified and characterized the P2X1 promoter utilized in MEG-01 cells and showed that binding of Sp1/3 and NF-1 to elements in the direct vicinity of the transcription start site is essential for basal transcription
- a GNAI2 -318 C>G SNP impairs transcriptional activity through specific binding of Sp1 and is associated with high SBP in Caucasians from Italy
- results suggest that p16 suppresses MMP-2 by blocking Sp1-mediated gene transcription
- Sp1 and Sp3 are principal mediators of hyaluronan synthase 2 constitutive transcription
- The effects of the EBV nuclear antigen 3C on the activities of Nm23-H1 via GATA-1 and Sp1 are reported.
- PADI3 expression is driven by Sp1 binding to the promoter region
- A pivotal role for Sp1 in the constitutive expression of the Protein S gene was demonstrated
- aryl hydrocarbon receptor-estrogen receptor 1/Sp1 cross talk is due, in part, to enhanced association of aryl hydrocarbon receptor and estrogen receptor 1
- Nevertheless, by gel shift assays, Sp1 and Sp3 were not found to be responsible for the protein-DNA complexes formed by the GC-rich sequence.
- findings suggest that activity at the HIV-1 promoter is influenced by phosphorylation of Sp1, which is affected by Tat and DNA-PK; interactions among TCF-4, Sp1 and/or Tat may determine the level of viral gene transcription in astrocytic cells
- Sp1 strongly contributes to the aberrant transcriptional response of transformed epithelial cells to TGFbeta stimulation.
- Sp1 epigenetic modifications have a critical role in regulation of the CLDN4 promoter in ovarian cancer cells
- cancer cells overexpressing Sp1 can avoid Sp1-induced apoptosis
- monoamine oxidase A activation of glucocorticoids and androgens is regulated differently by R1 and Sp1
- Sp1 proteins regulate COL11A2 transcription by binding to its proximal promoter and directly interacting with CBP, p300, and HDAC
- presence of p53 in a transcription complex of NPM and Sp1 or NF-kappaB at the promoter of the MnSOD gene was verified. p53 interacts with Sp1 to suppress expression of the MnSOD gene.
- These data indicate that IFN-gamma and TNF-alpha may repress the NHE3 promoter activity in C2BBe1 cells by PKA-mediated phosphorylation of Sp1 and Sp3 transcription factors.
- Sp1 is a strong activator, while Sp3 by its own is a weak activator of the DPYD promoter.
- study identifies, for the first time, GlcAT-I as a target of calcium-dependent signaling pathway and evidences the critical role of Sp1 transcription factor in the activation of GlcAT-I expression
- HSD17B2 expression in endometrial epithelial cells, and, therefore, estrogen inactivation, is regulated by SP1 and SP3
- "PP1-JNK" pathway plays a role in H(2)O(2)-induced Sp1 phosphorylation in lung epithelial cells
- heat-induced elevation of heat shock protein 70 was facilitated by Sp1 but blunted under low O-GlcNAc levels, suggesting that O-GlcNAc might upregulate the expression of heat shock protein 70 through thermoprotection of Sp1
- These results suggest that 1,25-dihydroxyvitamin D3 induces the formation of VDR/Sp1 complex and acts via a Sp1- and HDAC1-depedent pathway to inhibit p45Skp2 transcription.
- These studies have revealed a novel mechanism of Trichostatin A action through derecruitment of a repressor from the Luteinizing hormone receptor gene promoter in a PI3K/PKCzeta-induced Sp1 phosphorylation-dependent manner.
- Co-expression of Sp1 and DRIP-130 not only rescues KiSS-1 expression, but also induces an inhibition of the invasive and migratory behavior in highly metastatic melanoma cells
- WFS1 minimal promoter contains two DNA binding motifs (GC boxes) for the transcription factors Sp1/3/4 and binding of both Sp1 and Sp3 was demonstrated at both motifs in vitro and in vivo.
- role in IGF-IR gene transactivation
- In conclusion, we report that the transcription factor Sp1 involved in the p38MAPK-mediated control of p21WAF1 regulation on VSMC in response to BITC has now been identified.
- Activation of SP1 by insulin rapidly increases the expression of Pkrcd in skeletal muscles.
- Therefore, the global decrease in gene transcription frequently reported in aging cells and tissues could be attributed at least in part to the decrease in Sp1 level.
- Transcriptional activity of syt11 gene is affected by the number of 33-bp repeats, which include an Sp1 binding site, and associated with schizophrenia.
- results suggest that extracellular HIV-Tat induced IL-10 transcription in primary human monocytes is regulated by CREB-1 and Sp-1 transcription factors through the activation of calmodulin/CaMK-II-dependent p38 MAPK
- These findings indicate the dephosphorylation of c-Jun C terminus is required for the c-Jun/Sp1 interaction and reveal that PP2B plays an important role in regulating c-Jun/Sp1 interaction in phorbol 12-myristate 13-acetate -induced gene expression.
- Functional studies using RNA interference revealed that the silencing of Sp1 and KLF8 resulted in elevated level of gamma globin expression in K562 cells.These data indicate that Sp1 may play an important role in erythroid differentiation.
- Genes with both c-Myc-and sp1-binding sites have a distinct expression signature when compared to genes with either site alone.
- our results indicate that activator Sp1 and repressor RXRalpha:RARalpha act in concert to regulate MRP3 expression.
- Sp1 DNA binding activity is increased in cells exposed to hypo-osmolarity, which enhanced Sp1 binding to the endogenous p75NTR promoter. Sp1 is required for p75NTR expression induced by hypo-osmolarity.
- Here we report that the human SOX9 proximal promoter is also regulated by the cyclic-AMP response element binding protein (CREB) and Sp1.
- These data suggest Pdcd4 as a new negative regulator of intravasation, and qas the invasion-related gene u-PAR. It is the first study to implicate Pdcd4 regulation of gene expression via Sp1/Sp3.
- These results demonstrate that hypoxic condition-and high cell density-induced expression of Redd1 is mediated by coactivation of Sp1 and HIF-1alpha downstream of the PI3K/Akt signaling pathway.
- The role of HERG in the regulation of SP1, NF-kappaB and Nkx3.1 metabolism in neoplastic cells is reported.
- These data suggest that an intracellular calcium store independent PKC-Sp1 signaling pathway induces early keratinocyte differentiation through upregulation of TSG101.
- in human osteoblast-like MG63 cells, Sp1 and Sp3 stimulate basal PDPN transcription in a concerted, yet independent manner.
- These data suggests that, at least part of the biological actions of insulin-like growth factor-I in mammary gland cells may be mediated through BRCA1.
- Sp1 binding correlates with expression of the endogenous gene in vivo, correlating highly with alpha1dAR promoter methylation-dependent silencing of both episomally expressed reporter constructs and the endogenous gene.
- 2 beta1,6 N-acetylglucosaminyltransferase-I transcription is regulated by Sp1 in lymphocytes and epithelial cells
- Our data suggest that the differential regulation of NHE3 gene expression by NaB and IFN-gamma/TNF-alpha is mediated through alternative pathways that converge on Sp1/Sp3.
- Sp1 plays an important and positive role in ATP2A2 gene expression in normal human keratinocytes in vivo and in vitro.
- Regulator of basal MUC5B promoter activity in human airway epithelial cells.
- ATM-dependent Sp1 phosphorylation appears to be a global response to various DNA damage stress including viral DNA replication.
- EGFR activation in malignant gliomas can transcriptionally activate COX-2 expression in a process that requires p38-MAPK and Sp1/Sp3
- Laminin repressed transcription, directed by the alpha6 gene promoter, by altering the nuclear levels of Sp1 and Sp3
- Ets-1 enhances expression of the beta-1,4-GalT V gene through activation of the Sp1 gene in cancer cells
- Myc, Sp1, and HDAC1 coexist in the same DNA-protein complex at the HIV promoter. Short hairpin RNA inhibition of c-Myc reduces both c-Myc and HDAC1 occupancy, blocks c-Myc repression of Tat activation, and increases LTR expression.
- functional role of Sp transcription factors in upregulating P1-directed transcription was demonstrated
- In SW480 human colon cancer cells, we conclude that Sp1 mediated expression of the tumor metastasis protein, osteopontin, regulates in vitro functional correlates of tumor metastasis
- These data suggest that the regulation of COL1A1 gene transcription in human dermal fibroblasts involves a complex machinery that implicates at least three transcription proteins, hc-Krox, Sp1, and Sp3.
- Sp1 is a positive transcriptional regulator of T-box 21
- Data indicated that the synergistic activation of the human AM gene promoter by Sp1 and AP-2alpha may be mediated by the binding of Sp1 to the promoter region and the interaction with AP-2alpha, which binds to the promoter region.
- Sp1 protein enhances transcription of the hSlu7,Further, depletion of Sp1 affected alternative splicing
- MZF1 and Sp1/Sp3 binding to the promoter region drive the PADI1 expression in keratinocytes
- Sp1 functions as basal transcriptional regulator of human and rat MATE1 gene through two GC boxes. May be conserved among species. We have identified rSNP of hMATE1 gene (G-32A) (belonging to Sp1-binding site) that affects promoter activity.
- Data show that by interacting with promoter-bound Sp1 at both the proximal promoter region and at newly identified sites in a distal region (-2.4 kb), HNF4alpha1 activates the expression of p21.
- A pathway comprising PKCs>Raf-1>MEK-1>ERK-1/-2 mediates the effect of gastrin on the CgA promoter, and strongly suggests that enhanced phosphorylation of Sp1 and CREB is crucial for CgA transactivation through the G protein-coupled CCK-B/gastrin receptor
- Of two Sp1 transcription factor sites that bind the polycystic kidney disease 1 (Pkd1) proximal promoter, the Sp1-A site (the nearest Sp1 to the ATG start codon) is more important for activation and expression of of Pkd1.
- Expression of Sp1 and KLF6 is developmentally regulated, providing basis for further investigations on regulation of Sp1 and KLF6 gene during course of corneal development and in corneal diseases such as keratoconus.
- TsA increases 5-LO promoter activity by the enhanced recruitment of Sp1 and Sp3 to the 5-LO promoter
- Expression of IL-10 in lung macrophages stimulated by LPS depends on concomitant activation of MAP kinases, which control downstream signalling to Sp-1 transcription factor. Sp-1 is a key signalling pathway for IL-10 expression in lung.
- IGF-1 plays a role in maintaining muscle GLUT3 expression and basal glucose uptake via the transcriptional factor Sp1.
- MAZ and Sp1 play important roles on the transcriptional activation of the human edn promoter through specific binding to a 34-nt segment present in representative primate eosinophil rnase promoters.
- This report describes a new mechanism of glioma invasion involving a cooperative effort between E1AF and Sp1 transcription factors.
- HNF-4alpha plays a crucial role in human PZ gene expression in hepatocytic cells, and Sp1 is also important
- Role of transcriptional factors Sp1, c-Rel, and c-Jun in LPS-induced C/EBPdelta gene expression of mouse macrophages.
- the cooperative interaction of Sp1 and YY1 transcription factors is the critical event triggering the initiation of transcription of the MOR gene in lymphocytes
- elevated level of Sp1 observed in tissue extracts obtained from keloid tissue; inhibitors of Sp1-activated transcription reduced expression of extracellular matrix components in keloid fibroblasts
- Monocyte stimulation with microbial products up-regulated phosphorylated Sp1 nuclear content & Sp1 DNA-binding activity. Sp1 plays a key role in transcriptional regulation of MIF expression & MIF-dependent host antimicrobial innate immune defense.
- We conclude Sp1 directly regulates Lhx4 gene expression.
- Reduced hENT1-mediated adenosine transport in high D-glucose may result from increased Sp1 binding to SLC29A1 promoter down-regulating hENT1 expression.
- Interleukin-6 (IL-6) and/or soluble IL-6 receptor down-regulation of human type II collagen gene expression in articular chondrocytes requires a decrease of Sp1.Sp3 ratio and of the binding activity of both factors to the COL2A1 promoter
- Intact SP1-binding sites are essential for promoter activity, whereas EGR-1 suppresses the transcription of the human SREBP-1a promoter
- a clear link between Sp1-dependent RON tyrosine kinase expression and invasion of breast carcinoma cells.
- Sp1 is a novel ATM substrate that plays a role in the cellular response to DNA damage
- Sp1-mediated transcriptional regulation of NFBD1 plays an important role in the regulation of DNA damage response.
- Sp1 recruits ATF3, c-Jun, and STAT3 to obtain the requisite synergistic effect in neuronal injury through DINE neuronal injury-inducible gene
- Sp1/Sp3, but not EKLF, is involved in the activation of the GATA-1 erythroid promoter, and that histones H3 and H4 are highly acetylated in this promoter region for an actively transcribed GATA-1
- JNK1 activation is necessary to phosphorylate Sp1 and to shield Sp1 from the ubiquitin-dependent degradation pathway during mitosis in tumor cell lines.
- Our study may provide evidence that the Sp1 polymorphism may ameliorate the effects of HRT on bone mineral density suggesting some additional regimens may be used to support bone strength and to decrease osteoporotic fractures.
- This study provides the first insight into the mechanisms that control basal expression of human SNF2L gene.
- All 3 residues in Sp1 (Thr668, Ser670, and Thr681) are required for Sp1-dependent platelet-derived growth factor-D activation in response to angiotensin II
- Sp1 activation is an essential event for transcriptional activation of tissue factor.
- Chondroitin sulphate and its derived hydrolytic fragments (CSf) repress COL1A1 gene transcription through a -112/-61 bp sequence upstream the start site of transcription and imply hc-Krox and Sp1 transcription factors.
- Sp1 and Sp3 plays role in regulating the expression of human EC-SOD in the lung.
- SNP haplotype in the CES1A2 promoter affects the Sp1 binding and transcriptional regulation; it well agreed with the efficacy of the medication of imidapril.
- Sp1 and Sp3 over-expression activates CD97 promoter activity in HEK293 cells.
- Overexpression of either Sp1 or full-length Sp3 was shown to augment LAT promoter activity, while siRNA-mediated knockdown of each transcription factor was demonstrated to have an inhibitory effect.
- Stat1/3 directly controls promoter P1 of the CASR, and the Stats indirectly regulate promoter P2 of the CASR via Sp1/3 in response to IL-6
- FAK induces KLF8 expression in human ovarian cancer cells by activating the PI3K-Akt signaling pathway, leading to the activation of KLF8 promoter by Sp1
- Pyk2/ERK 1/2 mediate Sp1- and c-Myc-dependent induction of telomerase activity by epidermal growth factor
- A novel regulatory signaling mechanism of transcriptional control in which the LHR is derepressed through PKCalpha/ERK-mediated Sp1 phosphorylation, causing the release of HDAC1/mSin3A complex from the promoter.
- Elf-1 in combination with Sp1 and GABP reduced FcRgamma promoter activity.
- SP1-like sequences mediate human CASP3 promoter activation by p73 and cisplatin.
- acute loss of p53 in normal HKc induces EGFR expression by a mechanism that involves YY1 and Sp1 and does not require p53 binding to the EGFR promoter
- not only CREB but also Sp1 plays a critical role in regulating basal CD2AP promoter activity in renal tubular epithelial cells
- there is a functional Sp1-binding element in the t-PA promoter controlling quercetin induction via the p38 MAPK pathway
- This report implicates SP1 as a critical component of DNA demethylation-dependent up-regulation of TLR2 expression in cystic fibrosis epithelial cells.
- elements of regulatory pathways involving transcription factor Sp1 may be useful targets for therapeutic intervention to prevent or reverse AD.
- These results demonstrate that mammalian copper homeostasis is maintained at the hCtr1 mRNA level, which is regulated by the Sp1 transcription factor.
- functional interaction between PPARgamma and Sp1 in regulating p21Cip1/WAF1 in thyroid neoplasm
- AML1-ETO promotes leukemogenesis by blocking cell differentiation through inhibition of Sp1 transactivity.
- Transcription of the invasion suppressor, CRMP-1, is reciprocally regulated at the promoter region by C/EBPalpha and Sp1.
- Sp1/Egr1-repeat polymorphism in the ALOX5 promoter is not a genetic marker for the risk of developing late-onset Alzheimer disease
- The co-occupancy of ERalpha and Sp1 on TFF1 promoter remains at similar level with and without estrogen, while that of ERalpha and Sp3 increased in the presence of estrogen
- upon the NaBu stimulation, binding of Sp1 protein to ALAS2 promoter increased significantly, with concurrent increases in acetylation level of histone H3 and dimethylation level of H3-Lysine4 at ALAS2 promoter
- These results demonstrate the importance of Sp1 in the regulation of ZIP8 expression.
- Sumoylation of SP1 augments its degradation by changing the localization and increasing the SP1 proteolytic process.
- transcription factors Sp1 and p73 mediate p53-independent induction of PUMA following serum starvation to trigger apoptosis in human cancer cells.
- These results indicate that the hypoxia-induced activation of the XB-S promoter is regulated through dissociation of HDAC1 from an Sp1-binding hypoxia-responsive element site.
- Curcumin also decreased bladder tumor growth in athymic nude mice bearing KU7 cells as xenografts and this was accompanied by decreased Sp1, Sp3, and Sp4 protein levels in tumors.
- Activation of protein kinase G Increases the expression of p21CIP1, p27KIP1, and histidine triad protein 1 through Sp1.
- the basal activity of the human PRDM1 promoter, through which several factors, including SP1, SP3 and early growth response 1, modulate its expression through a conserved GC-box.
- These observations suggest that phosphorylated Sp1 might play a role in DNA repair at damage sites rather than functioning in transcriptional regulation.
- identify a Sp1 element and a reverse CCAAT box to be essential for basal Tbx2 promoter activity
- Sp1 is critical for sHBZ transcription, which accounts for the constitutive expression of the sHBZ gene
- Genome-wide occupancy of SREBP1 and its paergtners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes.
- Butyrate-induced phosphatase regulates VEGF and angiogenesis via Sp1
- Expression of Sp1 was post-transcriptionally up-regulated with increasing time in primary keratinocytes cultures.
- confirmed that downregulation of Sp1 resulted in an increased expression of WIF1 and decreased methylation of WIF1 promoter
- These results show that methylation, histone acetylation and Sp1 are important in the transcriptional regulation of the CIC proximal promoter.
- Sp1 and NFkappaB binding to the probes resembling their putative binding sites present in the S100A6 promoter was decreased in the presence of wild type p53.
- Sp1/Sp3 binding at the promoter of RIL gene prevents DNA hypermethylation.
- Different from ERbeta, p53 interacts with HDAC1 and CtBP1 and forms an inhibiting transcriptional complex that could compete for binding to Sp1 sites with ERalpha transcriptional complex and inhibit BRCA2 transcription more significantly
- Sp1 may be a key mediator of cell cycle associated changes in gene expression.
- study concludes that IL-1beta upregulates HKalpha transcription by inducing Sp1 binding to HKalpha Sp1 & NF-kappaB sites & that Helicobacter pylori perturbation of HKalpha expression is independent of Sp1-mediated basal HKalpha transcription
- These results suggest that alpha5 gene expression is likely dictated by subtle alterations in the nuclear ratio of TFs that either repress (NFI) or activate (Sp1 and AP-1) alpha5 transcription in corneal epithelial cells.
- NRAMP1 distal cis element binds Sp1 and is required for NRAMP1 myeloid regulation
- Proto-oncogene FBI-1 (Pokemon/ZBTB7A) represses transcription of the tumor suppressor Rb gene via binding competition with Sp1 and recruitment of co-repressors.
- These studies indicated that the TAF-Ialpha promoter is under the control of Sp1.
- Sp1 works together with p53 in the regulation of H2O2-induced PUMA expression and apoptosis in colorectal cancer cells.
- a novel Sp1-dependent regulatory pathway for CD39 indicate the likelihood that CD39 is central to protective responses to hypoxia/ischemia
- inhibition of either ERK or Sp1 suppressed M-CSF-induced VEGF at the mRNA and protein level.
- SP1 and MTF-1 represent new targets in the development of key therapeutics toward modulating the expression of the cellular prion protein and ultimately the prevention of prion disease
- Mutational analysis of the two Sp1 sites suggested their requirement for the promoter activityof SLC22A18.
- Cell confluency-induced Stat3 activation regulates NHE3 expression by recruiting Sp1 and Sp3 to the proximal NHE3 promoter region during epithelial dome formation.
- O-linked N-acetylglucosamines within the second serine/threonine-rich region of Sp1 interrupts a known interaction between Sp1 and Oct1
- Sp1 slightly positively regulate BRD7 promoter activity.
- overlapping E2F1/Sp1 site, being present in multiple copies in the p14ARF promoter, may serve as the targets for both E2F1 and Sp1, thereby playing a crucial role in response to some oncogenic signals and stimulators
- These data provide critical insights into the transcriptional regulation of the IP gene in human megakaryocytic and endothelial cells, identifying Sp1, PU.1 and Oct-1 as the critical factors involved in its basal regulation in humans.
- Mutation within a putative Sp1 binding site in BIRC5 promoter led to a sharp decrease of promoter activity.
- Constitutive transcription of chemokine CCL5 mRNA in natural killer (NK) cells is mediated through binding of the SP1 transcription factor to a site just upstream from the TATA box of the CCL5 gene.
- each Sp1 and NF-kappaB site differentially contributes to the regulation of HIV viral gene expression, and Sp1 sites functionally "dampen" transcriptional noise and thereby modulate the frequency and maintenance of this model of viral latency
- The results suggest that the overall tertiary structure formed by the three zinc fingers is essential for nuclear localization of Sp1 as well as dispersed basic amino acids within the zinc fingers region.
- binding of SP1 to the proximal promoter region stimulated the promoter activity and endogenous KCTD10 expression, whereas binding of AP-2alpha to this region showed opposite effects.