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Validated All-in-One™ qPCR Primer for SOD2(NM_000636.3) Search again
Product ID:
HQP017616
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
GC1, GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD
Gene Description:
superoxide dismutase 2
Target Gene Accession:
NM_000636.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.
Gene References into function
- Review: Contribution of proteomics to the molecular analysis of renal cell carcinoma with an emphasis on manganese superoxide dismutase.
- Cu,Zn-SOD and Mn-SOD are differently regulated by estrogen and progesterone in human endometrial stromal cells
- Polymorphism in MnSOD is associated with age among hispanics with colorectal carcinoma
- Increased expression of manganese superoxide dismutase is associated with that of nitrotyrosine in myopathies with rimmed vacuoles.
- AP-2 down-regulates transcription of the human SOD2 gene via its interaction with Sp1 within the promoter region.
- Transcription regulation of human manganese superoxide dismutase gene.
- Catalytic pathway of manganese superoxide dismutase by direct observation of superoxide.
- NF-kappaB-dependent MnSOD expression protects adenocarcinoma cells from TNF-alpha-induced apoptosis
- Manganese superoxide dismutase transgenic mice: characteristics and implications
- Nuclear factor-kappa B is required for tumor necrosis factor-alpha-induced manganese superoxide dismutase expression in human endometrial stromal cells.
- PKB-regulated Forkhead transcription factor FOXO3a (also known as FKHR-L1) protects quiescent cells from oxidative stress by directly increasing their quantities of manganese superoxide dismutase (MnSOD) messenger RNA and protein
- Val-Ala polymorphism in Mn-SOD influences neither susceptibility to alcohol-induced liver fibrosis nor alcohol-induced oxidative stress.
- Manganese superoxide dismutase expression within tumor cells is closely related to mode of invasion in human gastric carcinoma
- a new thiol-sensitive mutant form of the human mitochondrial enzyme
- Gene transfer of this enzyme extends islet graft function in a mouse model of autoimmune diabetes.
- This isoform, when expressed in PC-12 cells affects t-butylhydroperoxide-induced apoptosis differentially from its isoenzyme.
- In prostate cancer cells, one of the downstream mediators of the senescence-associated tumor suppression effect of mac25/IGFBP-rP1 is SOD-2.
- Replacement of His-30 with Val in human MnSOD results in a mutant with much decreased catalytic activity and highly susceptible to product inhibition compared to wild-type.
- protein whose expression is deregulated in the epidermis of the elderly
- It is indicated that polymorphic mutations of Mn-SOD exist in human normal cells and that the deletions might be obtained in the course of malignant transformation of OSC although decrease in Mn-SOD activity is not involved in the transformation.
- data suggest communication between the proximal promoter region and the TNF-responsive element which involves chromatin remodeling and histone acetylation during the induction process of Mn-SOD in response to TNF.
- MnSOD may be a tumor suppressor gene in human pancreatic cancer
- Higher frequencies of SOD2 allele Val and genotype Val/Val and of SOD3 allele Arg and genotype Arg/Arg were established for group DPN+. On this evidence, SOD2 and SOD3 were associated with DPN in DM type 1.
- Overexpression of the human MnSOD transgene in 32D cl 3 cells results in stabilization of the mitochondria and reduction in radiation-, TNF-alpha-, or IL-3 withdrawal-induced damage.
- no significant differences in the genotype, allele, and phenotype frequencies of MnSOD gene polymorphisms between patients with ankylosing spondylitis and controls
- Elevated expression of MnSOD in neuronal ceroid lipofuscinosis.
- the influence of a functional polymorphism of the dopamine D3 receptor, and its interaction with a Mn superoxide dismutase (MnSOD) polymorphism, in contributing to tardive dyskinesia in a chronic inpatient population with schizophrenia
- No association of the Ala-9Val MnSOD polymorphism to the development of breast cancer
- Results suggest that the failure of manganese-superoxide dismutase mRNA induction by oxidative stress in peripheral lymphocytes may be involved in the development of gastric cancer.
- Regulation of MnSOD by IL-1 in retinoic acid-differentiated neuroblastoma cells was mediated by the nuclear factor kappaB.
- Cu/Zn-SOD in cytosol and Mn-SOD in mitochondria each are capable of protecting HepG2 cells expressing CYP2E1 against cytotoxicity induced by pro-oxidants.
- The role of manganese superoxide dismutase (Mn SOD) genes polymorphisms in the pathogenesis of systemic lupus erythematosus (SLE).
- His30 and Tyr166 in wild-type Mn-SOD have roles in prolonging the lifetime of the inhibited complex
- CYP1A1 4887A may be a risk factor for the development of reactive arthritis, especially in the presence of Mn SOD 1183T/T
- an active site mutant of human manganese-superoxide dismutase has anti-proliferative functions and demonstrates the signaling role of MnSOD
- Increase in hydroxyl radical concentration in the extracellular space of muscles from wild-type mice after the contraction protocol most likely results from degradation of hydrogen peroxide generated by MnSOD activity.
- human SOD2 gene is induced by nucleophosmin and NF-kappaB
- functional polymorphisms in MTP and MnSOD may be involved in determining susceptibility of non-alcoholic steatohepatitis
- Review. Blood vessels express 3 isoforms of superoxide dismutase, 1 of which is manganese SOD in mitochondria. This review will focus mainly on the role of individual SODs in relation to endothelium under normal conditions and in disease states.
- Neoplastic cells in breast carcinomas retain their capability to produce MNSOD, thus are protected from possible cellular damage by reactive oxygen species. MNSOD content varies according to the degree of differentiation of breast carcinoma.
- MnSOD Ala allele polymorphism increases risk of breast cancer in cigarette smokers.
- Finds homozygous variant MnSOD genotype is associated with increased lung cancer risk among individuals with zero or "low" asbestos exposure score and no association among the "high" AES group.
- Vascular endothelial growth factor is coupled to Mn-SOD expression through growth factor-specific reactive oxygen species.
- results indicate that the PKC pathway leading to SOD2 induction proceeds at least in part through NF-kappaB
- Although (MnSOD) in DEV cells was significantly increased in acute infection with viral and nutritional stress, in persistent infection and nutritional stress, the expression of the MnSOD was drastically downregulated.
- Cu, Zn- and MnSOD levels were significantly increased in frontal cortex and substantia innominata of the index group in schizophrenia
- Iindoleamine 2,3-dioxygenase, manganese superoxide dismutase, and interleukin-1 receptor antagonist protein transferred to isolated NOD mouse donor islets protect islet grafts from diabetogenic T cells.
- Overexpression of manganese superoxide dismutase promotes the survival of prostate cancer cells exposed to hyperthermia
- Data show that TNF-alpha increased the level of two antioxidant enzymes, thioredoxin and manganese superoxide dismutase, by an NF-kappaB-dependent mechanism in Ewing sarcoma cells.
- the functional A16V MnSOD polymorphism affects the risk of cardiomyopathy related to iron overload and possibly to other known and unknown risk factors, and may represent an iron toxicity modifier
- Reduced the oriental medicine-induced cytotoxicity and decreased the number of the oriental medicine-induced apoptotic cells.
- Sod2 can serve as an alternative physiological source of the potent signaling molecule, H2O2 via PTEN oxidation
- HIV-1 Tat regulates the SOD2 basal promoter by altering Sp1/Sp3 binding activity
- The association of the manganese superoxide dismutase polymorphisms at -102 C>T and the -9 T>C were not found to be associated with gastric cancer in a Polish case-control study.
- MnSOD protects the redox-sensitive checkpoint which regulates the progression of fibroblasts from G0/G1 to S-phase.
- hydrogen peroxide overload on p53-independent pathway due to MnSOD overexpression plus buthionine sulfoximine might increase apoptosis frequency without acceleration of apoptotic process of each cell
- Mitochondria-localized MnSOD dramatically reduced the release of cytochrome c to cytosol by proline oxidase in colorectal cells.
- Study findings suggest a novel mechanism by which Tat modulates the repression of the MNSOD gene and establishes a link between HIV infection and liver cancer.
- Polymorphism in the manganese superoxide dismutase gene is associated with breast cancer
- Altered nuclear expression of Mn-SOD parallels, together with changes in other elements of the antioxidant protective system, the loss of differentiation occurring during the progression of thyroid tumors.
- The data show that MnSOD affects sensitivity of cells to Pc 4-PDT-initiated apoptosis, and partly ceramide accumulation, suggesting that the processes are superoxide-mediated.
- Epigenetic silencing of SOD2 in multiple myeloma increases cell proliferation.
- uncoupling of the electrochemical gradient by increased MnSOD activity gives rise to p53 up-regulation
- Protein kinase D-mediated MnSOD expression promotes increased survival of cells upon release of mitochondrial reactive oxygen species
- HIF-1alpha accumulation and VEGF expression could be modulated by the antioxidant enzyme MnSOD.
- the increase in Mn-SOD expression in ovarian cancer is a cellular response to intrinsic ROS stress
- Endogenously generated matrix superoxide does not regulate UCP activity and in vivo energy expenditure; transgenic Sod2 demonstrates absence of effect on any UCP activities, and transgenic mice display normal resting metabolic rates
- Data show that manganese-dependent superoxide dismutase levels are much higher in the hippocampi of Alzheimer's diseased brains than in non-diseased control brains.
- Ala-9Val polymorphism does not seem to be a significant predisposing factor for bronchial asthma in the Czech population
- We did not observe an association between the C47T polymorphism in the MnSOD gene and survival
- the Ala-9Val polymorphism in the MnSOD gene is not associated with genetic susceptibility in Alzheimer's disease patients
- SODs might be important in the pathogenesis of NP; however, the roles these SOD isoforms play in both normal nasal mucosa and NP require further clarification.
- presence of p53 in a transcription complex of NPM and Sp1 or NF-kappaB at the promoter of the MnSOD gene was verified. p53 interacts with Sp1 to suppress expression of the MnSOD gene.
- May be a genetic factor regulating the cellular redox state in determining the outcome of leukemia chemotherapy
- that the localization of Cu/Zn- and Mn-SODs in adrenal tissues reflects the specificity of the adrenal cells that produce the tissue-specific hormones
- The results suggest that Ala/Val polymorphism of the SOD2 gene could be associated with the risk of developing methamphetamine psychosis.
- These studies show that residues at the dimeric interface, such as Tyr169, have significantly less conformational freedom or mobility than do residues at the tetrameric interface, such as Tyr45.
- determination of MnSOD polymorphism and different genotypes of MnSOD associated with its antioxidant enzyme activity in chronic pelvic pain syndrome patients
- The MnSOD Ala-9Val polymorphism may contribute to an increase in breast cancer risk in the context of high alcohol consumption, however the polymorphism is not an overall risk factor for breast cancer in this primarily premenopausal population.
- Polymorphisms in the GPX-1 and MnSOD genes may have a role in development of breast cancer
- Phosphorylation of ERK1/2 led to an activation of NFkB, and increased mRNA levels of the antioxidant enzyme manganese-superoxide dismutase (MnSOD).
- the MnSOD Val16Ala variant allele may have a role in preventing development of breast cancer in women who never breast fed
- Mean relative levels of SOD2 & its mRNA were significantly decreased in women > or =38 years, which may account for granulosa-cell changes associated with reproductive aging.
- Binding of NF-kappaB members to the MnSOD gene leads to the induction of MnSOD mRNA and protein levels, leading to subsequent protection against oxidative stress-induced neuronal injury.
- Systemic activity of the enzymatic antioxidants (CuZn/SOD, MnSOD, GSH-Px, and CAT) as well as level of lipid peroxidation determined by MDA may not be increased in the course of immune-inflammatory processes associated with chronic idiopathic urticaria.
- No significant association between MnSOD gene polymorphism and the risk of skin cancer.
- Smoking and homozygosity for the MnSOD Val allele is associated with an increased risk of diabetic nephropathy.
- Activation of pattern recognition receptors of the innate immune system results in strong upregulation of SOD2 gene expression suggesting that SOD2 protects macrophages from oxidative stress during microbial infection.
- SNPs involving the GSTP1, MnSOD and GPX2 genes were not associated with Barrett's esophagus or esophageal adenocarcinoma
- An increased risk of pleural mesothelioma was found in those with the Ala/Ala genotypes at codon 16 within MnSOD.
- Extracellular SOD, endothelial NOS, and inducible NOS gene polymorphisms do not constitute a risk factor for developing BD (Behcet disease) in Japan.
- 10T/9T polymorphism in the SOD2 intron 3 results in alternative splicing of exon 4; over-representation of 9T alleles indicates that this polymorphism may be involved in the altered susceptibilities to disorders that are more common in African-Americans
- Ala-encoding MnSOD allele is represented equally in controls and patients with HCV-related cirrhosis, and it does not significantly influence the risks of liver iron overload, HCC, or death.
- No association between SOD polymorphism and bladder cancer incidence.
- Under a dominant model, family-based association tests showed significant evidence for association of Alzheimer's disease with the first three loci of superoxide dismutase 2 in two candidate gene sets of families.
- study of the relationship between genetic polymorphisms of MnSOD, NQO1, GSTM1 and GSTT1 and the susceptibility to drug-induced liver injury (DILI); MnSOD mutant C allele may increase the susceptibility to DILI
- The common Val16Ala MnSOD polymorphism does not confer increased or reduced risk of lung cancer in Chinese in Hong Kong
- No major modifying role for the Mn-SOD gene polymorphism in patients with pseudoexfoliation syndrome.
- UDN glycoprotein regulated SOD2 activity and is a potential modulator of inflammatory signal pathways in a lipopolysaccharide-treated colonic cancer cell line.
- mutation of the MnSOD gene may be an important risk factor for prostate cancer
- MnSOD plays a role in regulating tumor cell growth and invasive properties of estrogen-independent metastatic breast cancer cells via hydrogen peroxide.
- Polymorphisms of MnSOD and GSTP1 are not associated with chronic alcoholic pancreatitis.
- analysis of superoxide dismutase and catalase polymorphisms in smokers with COPD
- Significant difference in the genotype frequency between healthy subjects and patients with sepsis.
- The distribution of the MnSOD genotypes and alleles was not significantly different between patients and controls. Logistic regression analysis also failed to reveal any association between MnSOD genotypes and TD.
- that an O(2)(*-) signaling pathway regulates NAC-induced G(1) arrest by decreasing cyclin D1 protein levels and increasing MnSOD activity
- During neoplastic transformation of cirrhotic liver, an increase in MnSOD activity may occur already during the precancerous phase, making this enzyme a probable malignancy-associated parameter.
- Manganese superoxide dismutase gene polymorphisms is associated with urolithiasis
- These results suggest that the Ala variant of SOD2 is associated with moderately increased risk of prostate cancer.
- p50 shows a negative effect on MnSOD induction upon repeated applications of TPA and provide an insight into a cause for the reduction of MnSOD expression during early stages of skin carcinogenesis.
- Overexpression of MnSOD at moderate levels is able to protect cells from TRAIL-induced apoptosis.
- Data suggest that MnSOD may be useful in treating HER2/neu-mediated human breast tumor malignancy.
- Increased activity of superoxide dismutase in advanced stages of head and neck squamous cell carcinoma with locoregional metastases
- the combined genotype group of SOD2-01 and GSTP1-01 was an independent predictor of progression free survival and breast cancer specific survival in patients with metastatic breast cancer
- study concludes that the Val9Ala MnSOD polymorphism does not influence ovarian cancer risk or survival
- Epigenetic silencing of SOD2 constitutes one mechanism leading to the decreased expression of MnSOD observed in many breast cancers.
- These findings suggest that the association between increased Sod2 activity and poor prognosis in cancer can be attributed to alterations in their migratory and invasive capacity.
- Findings provide further evidence of an association between the Ala-9Val MnSOD polymorphism and HCC occurrence in hepatitis C virus-infected Moroccan patients.
- emphasize the role of residue 66 in catalysis and inhibition and provide a structural explanation for differences in catalytic properties between human and certain bacterial forms of MnSOD
- concludes that the VV genotype of the V16A polymorphism of the manganese superoxide dismutase(Mn-SOD) gene was associated with diabetic retinopathy in Caucasians with type 2 diabetes
- in systemic lupus erythematosus patients, MnSOD2 (superoxide dismutase 2)Val/Val genotype was associated with Raynaud's phenomenon and contributed to immunologic manifestations and autoantibody production
- MnSOD was overexpressed in ESCC and its up-regulation in esophageal cancer cells was associated with apoptosis resistance.
- Thus, long-term exposure of human cells to RES results in a highly specific upregulation of MnSOD, and this may be an important mechanism by which it elicits its effects in human cells.
- Meta-analysis shows a protective effect against antipsychotic-induced tardive dyskinesia for Ala9Val and Val carriers.
- Polymorphisms of the inflammatory pathway genes MPO -G463A and SOD2 Ala16Val are associated with elevated pancreatic cancer risk
- Kinetic mechanism of manganese-containing superoxide dismutase.
- Manganese superoxide dismutase (MnSOD) activity has been used as an index of mitochondrial antioxidant defence in type 2 diabeates.
- MnSOD is not associated with risk of prostate cancer, but MnSOD T to C (Val-9Ala, rs4880) polymorphism modifies associations between risk of clinically aggressive prostate cancer and dietary iron intake
- Glu162 at the tetrameric interface in human MnSOD supports stability and efficient catalysis and has a significant role in regulating product inhibition
- Polymorphisms of CuZn-SOD, MnSOD, GSTM1 and GSTT1 in the placental tissue were not associated with preeclampsia.
- Overexpression of MnSOD in breast epithelial cells alters stabilization of HIF-1 alpha under hypoxic conditions.
- genotype distribution of the SOD2 in patients with diabetes mellitus can differ from nondiabetic individuals
- Shear stress influences spatial variations in vascular Mn-SOD expression with implications for LDL nitration.
- results thus indicated that elevated SOD2 levels is associated with lymph node metastasis in oral squamous cell carcinoma and may provide predictive values for diagnosis of metastasis
- lung cancer cases and 141 controls were evaluated for the 58T > C and 60C > T SNPs in exon 3. All were of the wild-type homozygous genotype for both 58C and 60T in exon 3.
- The results suggest that (estrogen-activated) MnSOD plays an important role against mitochondrial oxidative stress by diminishing reactive oxygen species, thus promoting cell survival.
- The MnSOD AA genotype was associated with prostate cancer in our study
- The evidence for the risk effect of the C allele of SOD2 rs4880 for exudative age-related macular degeneration seems to be very fragile.
- single nucleotide polymorphisms in the SOD2, XRCC1 and XRCC3 genes are associated with the development of late radiation injury in patients treated with radiation therapy for prostate adenocarcinoma
- These data suggest that Ala/Ala MnSOD genotype in the Macedonian population could have an influence on early onset of prostate cancer, but no impact on the subsequent development of the disease.
- MnSOD alanine16valine polymorphism may be associated with development of type 2 diabetes among Japanese-Americans.
- common variants in the SOD2, SOD3, and CAT genes may influence brain tumor risk.
- inactivation of DJ-1 causes decreased expression of the human MnSOD
- the Ala-MnSOD variant favors hepatic iron accumulation by modulating the expression of proteins involved in iron homeostasis
- SOD1 and SOD2 expressions demonstrate how sex and daytime, merit being controlled when human gene expression analyses are evaluated, particularly within the framework of clinical trials or cohort studies.
- when antioxidant status is low, the MnSOD Ala/Ala genotype may be associated with an increased risk of aggressive prostate cancer.
- down-regulation of SOD2 induces AR activity in a reactive oxygen species-dependent manner
- results indicate that epigenetic silencing of SOD2 could be facilitated by changes in histone modifications and represent one mechanism leading to the altered expression of MnSOD observed in many breast cancers
- Increased superoxide dismutase 2 expression is associated with multiple sclerosis lesions.
- The combined genotypes of T/T in NQO1 Pro187Ser and Val/Val in MnSOD Ala-9Val polymorphisms were found to be independently associated with a significantly higher risk of TD.
- The authors identified manganese superoxide dismutase (MnSOD), a mitochondrial antioxidant and an important antiapoptotic enzyme, as the protein most strongly upregulated by vFLIP/K13 in endothelial cells.
- meta-analysis on total of 7,366 cancer cases and 9,102 controls from 13 published case-control studies showed no overall association of the MnSOD Val(16)Ala polymorphism either with breast cancer risk or for cancer risk as such [review]
- the high level of MnSOD observed in aggressive cancer cells may be due, in part, to the absence of AP-2 transcriptional repression
- No indication of an increase in DNA lesions due to depletion of SOD2 was found.
- the Val/Val genotype of SOD2 may be associated with lung cancer in a Turkish