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Validated All-in-One™ qPCR Primer for FSCN1(NM_003088.3) Search again
Product ID:
HQP017585
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
FAN1, HSN, SNL, p55
Gene Description:
fascin actin-bundling protein 1
Target Gene Accession:
NM_003088.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- fascin is expressed in cutaneous CD30+ lymphoproliferative disorders and that it is a candidate marker of disease progression
- Fascin is upregulated in invasive and more aggressive NSCLC, being an independent prognostic predictor of unfavourable clinical course of the disease
- Fascin regulatory sequences are found solely in the 5'-flanking promoter region; the promoter exerts robust activity in dendritic cells (DC) and a fascin-positive neuronal cell line; promoter activity in DC markedly increases with maturation of DC.
- Fascin-1 plays an essential role in polarity of actin bundles in filopodia. In the in vitro motility assay, actin/fascin bundles slid as fast as single actin filaments on myosin II and myosin V
- fascin is an actin-bundling motility protein with a role in progression of hormone receptor-negative breast cancer
- In conclusion, this research supports FSCN1 as a novel marker of NT2 neuronal differentiation and the possible role of CBP in its regulation.
- Over expression of fascin is associated with basal-like phenotype and BRCA1-associated node-negative invasive breast carcinomas
- Higher fascin scores in fascin in renal cell carcinoma were significantly correlated with higher T and N stages and nuclear grade
- Fascin is upregulated in a proportion of adenomas, where its expression is often focal. Fascin may have prognostic value as an early biomarker for more aggressive colorectal adenocarcinomas.
- LXR is a potent modulator of dendritic cells maturation and function mediated in part by blocking the expression of fascin.
- Higher expression of fascin-1 correlates significantly with tumor grades and stages in colorectal adenocarcinomas.
- Higher intensity of cortactin and fascin-1 staining correlated directly with more-advanced cancer stages (TNM) and inversely with survival rates.
- Fascin is a novel target of beta-catenin-TCF signaling and is expressed at the invasive front of human colon cancer
- Analysis of fascin bundling indicated that filopodia are semiordered bundles with one bound fascin per 25-60 actin monomers.
- This study demonstrates a role for fascin in glioma cell morphology, motility, and invasiveness.
- Fascin may be a novel marker to distinguish thyroid carcinoma from benign lesions
- The fascin-1 expression did not correlate with the depth of tumor invasion or with the urinary bladder tumor recurrence or progression.
- Myosin X filopodial localization is perturbed in fascin-depleted HeLa cells, demonstrating that fascin bundles also direct motility in vivo.
- Rac regulates the interaction of fascin with active PKC.
- Cortactin, fascin-1 and EGFR have roles in progression of ovarian carcinomas
- Fascin overexpression may be a marker of aggressive urothelial carcinomas.
- Cadherin-11 and fascin might be useful markers for recurrence of pleomorphic adenomas.