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Validated All-in-One™ qPCR Primer for SLC22A3(NM_021977.3) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. [provided by RefSeq].
Gene References into function
- The regulation of EMT-mediated transport by second-messenger phosphorylation/dephosphorylation mechanisms has been characterized in stably transfected HEK293 cells with tritiated 1-methyl-4-phenylpyridinium as substrate.
- EMT efficiently translocates agmatine and must be considered for the control of agmatine levels.
- Genetic variation of EMT was studied in Caucasians.
- EMT is expressed in the area postrema of rat brain and may play a role in physiological functions of this circumventricular organ such as emesis, food intake and the regulation of cardiovascular functions.
- organic cation transporter EMT mRNA was mainly detected in the intra lobular septa and also expressed in scattered cells of placental vessel adventitias with lower expression of EMT mRNAs in pre-eclamptic placentae
- Ranitidine and famotidine elicited differential inhibitory activities on SLC22A3.
- These results suggest that polymorphisms of SLC22A3 are related to the development of polysubstance use in Japanese patients with methamphetamine (MAP) dependence.
- PMAT, EMT, and OCT2 transporters are expressed in the endometrial stroma and can potentially regulate reuptake of monoamines in general and histamine in particular.
- rare mutations in the EMT gene suggest a causative or modulating role in genetic subtypes of obsessive-compulsive disorder
- Confirm the usefulness of Caki-1 cells as a proximal tubule model system for investigations of OCT3.
- We conclude that the proliferation of immature germ cells in GB may be due to an interaction between OCT3/4 and accumulated beta-catenin in the nuclei of the immature germ cells.