|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for BMP7(NM_001719.2) Search again
Product ID:
HQP017467
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
OP-1
Gene Description:
bone morphogenetic protein 7
Target Gene Accession:
NM_001719.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth.
Gene References into function
- Transforming growth factor-beta1 supports the rapid morphogenesis of heterotopic endochondral bone initiated by human osteogenic protein-1 via the synergistic upregulation of molecular markers
- age related decline in level may contribute to the elevated susceptibility of cartilage to the degenerative process.
- crystal structure of the antagonist Noggin bound to BMP-7, which shows that Noggin inhibits BMP signalling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors
- BMP-7 signaling is inhibited by glypican 3 in hepatocellular carcinoma cells
- BMP-7 inhibits constitutive and IL-1 beta-induced expression of monocyte chemoattractant protein-1 in mesangial cells, partly through suppression of c-Jun N-terminal kinase activity and AP-1 binding activity.
- crystal structure of BMP7 in complex with the extracellular domain (ECD) of the activin type II receptor
- IGF-1 and OP-1 could be key physiological regulators of MMP-13 gene expression
- In pulmonary artery smooth muscle cells from pulmonary hypertension patients, the BMP-2- or BMP-7-induced apoptosis was significantly inhibited compared with PASMCs from patients with secondary pulmonary hypertension.
- BMP-7 is expressed in various breast cancer cell lines in a cell line-specific manner
- BMP-7 plays an important role in the regulation of anti-inflammatory response in the adult gut tissue.
- Overall decrease in endogenous OP-1 in degenerated and OA tissue suggests that OP-1 could be one of the factors responsible for normal homeostasis and matrix integrity in cartilage.
- Modulation of endogenous osteogenic protein-1 (OP-1) by interleukin-1 in adult human articular cartilage.
- OP-1 stimulated cell proliferation and mRNA expression of several biochemical markers in this ligament cell culture model
- In the extraskeletal model, newly formed bone was evident in the presence of rhBMP-7
- results suggest expression of the Ihh gene, which contains multiple Smad binding sites, may be finely regulated by a gradient of bone morphogenetic protein BMP7
- Stimulation of kidney cells with BMP-7 induced hyaluronan synthase 2 mRNA expression and decreased the expression of Hyal1 and Hyal2.
- The effect of BMP-7 on TGF-beta1 synthesis in TNF-alpha-stimulated cells was abrogated by disruption of CD44-hyaluronan interactions, suggesting that it was due to increased monocyte binding to hyaluronan on the cell surface.
- To analyze the expression of bone morphogenetic proteins (BMPs) in prostate and breast cancers with established metastasis in bone
- the prodomain of BMP-7 has a role in targeting the BMP-7 complex to the extracellular matrix
- BMP7 induced Smad phosphorylation in a dose-dependent manner, with Smad activation clearly demonstrable in prostate adenocarcinoma.
- BMPs influence the formation of the osteolytic prostate cancer metastases, and treatment modalities that inhibit BMP activity may limit the progression of the lytic component of prostate cancer metastases.
- Our results are suggesting a possible functional role for BMP7 in breast cancer development.
- Measurement of OP-1 in joint fluid may have value in the clinical evaluation of joint diseases.
- BMP7 could restore the homeostatic balance of pSmad signaling found in normal kidneys, thereby preventing or reversing the development of chronic allograft nephropathy.
- Topical and differential expression of BMP-2/4 and BMP-7 mRNA and protein was found in bursa tissue.
- BMP-7 expression in the adult human kidney appears to be more restricted than in the fetal situation and predominantly found in the distal nephron.
- Osteogenic protein-1 (OP-1 has shown particular potential in clinical trials as a bone graft substitute .
- Recombinant human BMP-7 plays a role in the migration of bone-forming cells.
- maintenance of renal BMP-7 during the evolution of diabetic nephropathy reduces diabetic renal injury, especially podocyte dropout. The findings also establish a role for endogenous glomerular BMP-7 as an autocrine regulator of podocyte integrity in vivo
- BMP7 expression was almost completely absent at the invasive margin of esophageal tumors, but present in the central area. It may antagonize TGF-beta1's role in invasiveness.
- strong BMP staining is seen in maturing chondrocytes, and thus may play a role in chondrocyte differentiation and/or apoptosis; BMP release by osteoclasts may promote osteoblastic differentiation at sites of bone remodeling
- These results indicate that mutations are rare in FGF8 and FGFR2 in hypospadias, but gene variants may influence the risk.
- Immunohistochemistry of cirrhotic human liver demonstrated upregulated BMP7 protein expression in hepatocytes as compared with normal human liver.
- Proinvasive activity of BMP7 through SMAD4/src-independent and ERK/Rac/JNK-dependent signaling pathways in colon cancer cells is reported.
- BMP7 promotes the adipogenic and not the osteogenic or chondrogenic lineage development of human stem cells
- induction of BMP7 expression is frequent in melanomas and may serve as a novel prognostic marker of progression in melanoma patients
- BMP-7 was more potent than BMP-2 in inducing chondrogenesis, but the properties of the differentiated tissue were similar in each case
- The presence of BMP-7 and IL-1Ra antagonized the anti-anabolic effects of lipopolysaccharide
- Expression of BMP-4 and BMP-7 and their receptors in human ovaries from fetuses as well as adults.
- Approximately half of the prostate tumors displayed increased copy numbers of the BMP2, BMP5, BMP7, and UC28 gene loci, which may account for their abnormal gene expression patterns in neoplastic prostate tissue.
- Results suggest that bone morphogenic protein 7 controls and preserves the epithelial phenotype in the human prostate and underscore a decisive role of the tumor microenvironment in mediating the therapeutic response of BMP7.
- In breast cancer, bone morphogenetic protein 7 (BMP7) is a novel target gene regulated by the p53 family and mediates the cell survival function of the basal physiologically relevant level of p53.
- BMP-7 but not BMP-2 and BMP-4 prevents the loss of primitive hematopoietic progenitor cells cultured in SFM plus SF3.
- This screen identified a bone morphogenetic protein (BMP) antagonist, SOSTDC1 (sclerostin domain-containing-1) as down-regulated in kidney tumors.
- Significantly higher BMP7 expression is associated with colorectal cancer
- Allergen challenge was associated with marked and sustained up-regulation of BMP-7 in airway epithelium and infiltrating inflammatory cells
- DeltaNp63/BMP-7 signaling pathway modulates wound healing process through the regulation of matrilin-2.
- Expression of BMP1, BMP6, BMP7, and BMP-receptor 2 was significantly increased in advanced stages of myelofibrosis compared and enhanced levels of BMP6 expression were already evident in prefibrotic stages of primary myelofibrosis.
- This study was initiated to examine the effects of BMP-7 (also called Osteogenic Protein-1) on the temporal and spatial expression patterns of other BMPs and the BMP receptors in cell cultures of adult rat Achilles and Patellar tendons.
- BMP-2/4 and BMP-6/7 differentially utilize cell surface receptors to induce osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells
- Overproduction of BMP-7 was noted in ankylosing spondylitits(AS) patients and serum BMP-7 levels reflected radiologic damage observed in AS
- BMP-7 ameliorates IgA nephropathy-derived polymeric IgA-induced TNF-alpha and TGF-beta synthesis in human mesangial cells through multiple mechanisms involving inhibitory Smads and PPAR-gamma.
- Promise for therapeutic utility of rhBMP7 or small molecule BMP7 agonists in patients with chronic kidney diseases,[REVIEW]
- A significant elevation in expression of BMP7 in oral squamous cell carcinoma cells was seen in carbon and neon-irradiated cells.
- acts as an autocrine growth inhibitor in melanocytic cells; advanced melanoma cells may escape from BMP7-induced inhibition through concomitant aberrant expression of Noggin
- Results showed that type II receptors, such as BMP receptor II, activin receptor IIA, and activin receptor IIB, competed with the pd for binding to the growth factor and displaced the prodomain from the complex.
- BMP7 can promote and inhibit cell growth in breast cancer cell lines and, in a suitable environment, can also considerably induce breast cancer cell migration and invasion
- BMP-7 has no anti-fibrotic effect in lung or skin fibrosis either in vivo or in vitro
- IL1B can affect all the key steps in OP-1 signaling in chondrocytes