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Validated All-in-One™ qPCR Primer for SLC11A1(NM_000578.3) Search again
Product ID:
HQP017463
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
LSH, NRAMP, NRAMP1
Gene Description:
solute carrier family 11 member 1
Target Gene Accession:
NM_000578.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease.
Gene References into function
- The data supports a role for NRAMP1 in the antimicrobial defenses of human neutrophils.
- NRAMP1 gene promoter polymorphism could influence the radiological severity of rheumatoid arthritis and disease susceptibility, particularly in individuals lacking HLA-linked risk factors.
- Study of the CTLA-4 gene and type 1 diabetes showed that the CTLA-4 gene significantly contributed to the development of type 1 diabetes, whereas NRAMP1 had an additional effect on the onset of type 1 diabetes in the young population.
- results indicate that variant alleles in the Nramp1 gene are associated with increased mycobacterial replication rather than susceptibility for tuberculosis and may thus confer increased risk of severe disease
- The 469 + 14 G/C (INT4), 1465 - 85 G/A, and C274T polymorphisms of NRAMP1 were analyzed in ethnic Russians with (N = 58) or without (N = 127) tuberculosis. On evidence of allele and genotype frequencies, none of the polymorphisms was associated with TB
- associations/linkages of SLC11A1 with human disease and how these relate to functional promoter region polymorphisms
- Role in susceptibility to Kawasaki disease.
- Variations of the Nramp1 gene affects susceptibility to visceral leishmaniasis.
- The NRAMP1 genetic polymorphisms were closely related to tuberculous pleurisy.
- Polymorphisms of D543N and 3'UTR locus in NRAMP1 gene might affect their susceptibility to pulmonary tuberculosis in Chinese Han population.
- Polymorphisms in the VDR and NRAMP1 gene are statistically associated with susceptibility to pulmonary tuberculosis in the Chinese Han population.
- demonstrated significant differences in the ability of polymorphic alleles to regulate gene expression upon iron loading and addition of exogenous stimuli
- no evidence of association between SLC11A1 polymorphisms and multiple sclerosis susceptibility in the Spanish population.
- Existence of a locus at or near SLC11A1 that is a strong susceptibility factor for JIA in Finnish patients.
- allele 3 of SLC11A1 at the functional promoter region repeat polymorphism was significantly associated with sarcoidosis
- promoter polymorphism exhibited an interaction with the lepromin response, suggesting a susceptibility to leprosy
- Lack of association of SLC11A1 as an inflammatory bowel disease candidate gene.
- Genetic variants of NRAMP1 may have an effect on bacilli growth and on outcomes of pulmonary tuberculosis, but not on susceptibility to M. tuberculosis infection.
- Genetic variation in both the promoter region and intron 1 of the SLC11A1 gene is associated with esophageal cancer susceptibility
- Allele 2 associated with low SLC11A1 and protection against rheumatoid arthritis. Negative association of allele 2 with autoimmune type 1 diabetes suggests less active immune system in subjects with allele 2 may protect from autoimmune diseases.
- NRAMP1 is not likely to be a major contributor to the genetic etiology of asthma and asthma-related phenotypes.
- Iron dysregulation mediated by allelic effects of SLC11A1 may contribute to inflammatory bowel disease susceptibility.
- NRAMP1 823C/1703G/1729+del 4 TGTG+ haplotype is associated with susceptibility to rheumatoid arthritis
- Gene expression of SLC11A1 is regulated by HuR.
- This work examined the polymorphism of the human NRAMP1 gene in 65 patients with brucellosis and 89 healthy controls and found no significant differences in the alleles studied.
- A genetic polymorphism in the SLC11A1 gene plays a role in susceptibility to develop Buruli ulcer, with an estimated 13% population attributable risk.
- We identified a novel trinucleotide (ATA)n repeat polymorphism in intron 8 of SLC11AI. We found no significant association between this marker and pulmonary TB.
- Association between SLC11A1 and Tuberculosis, particularly to the common pulmonary form.
- Genetic variation in NRAMP 1 may affect susceptibility to and increase risk for tuberculosis in Taiwanese aboriginals
- An association was found between 3'-UTR polymorphisms of Slc11a1 and incidence of PTLD after liver transplantation.
- The allele 3 and the genotype allele 3/allele 3 of the 5'-promoter (GT)n in the SLC11A1 gene may have a protective effect for the development of Behcet's disease in the Korean population.
- While Crohn's disease was strongly associated with NRAMP1, NRAMP1 polymorphisms themselves were not correlated.
- The SLC 11A1 274T 823C 1703G 1729+55 del 4 TGTG+ haplotype is associated with the development of reactive arthritis in Taiwan.
- There is an increased risk of pulmonary tuberculosis in Chinese iron miners exposed to silica dust carrying both the NRAMP1 D543N G/G and NRAMP1 INT4 G/C+C/C genotypes.
- The NRAMP1 genes are not associated with susceptibility to tuberculosis in Thais.
- role SLC11A1 in linking infections, autoimmunity and cancer [review]
- We conclude that the difference in SLC11A1 promoter polymorphism plays no role in Crohn's disease in Ashkenazi Jews.
- human natural resistance-associated macrophage protein 1 gene might be involved in susceptibility to pulmonary Mycobacterium avium complex infection
- Iron deficiency and NRAMP1 polymorphisms (INT4, D543N and 3'UTR) do not contribute to severity of anaemia in tuberculosis in the Indonesian population
- hypoxia-inducible Factor 1 (HIF-1) regulates allelic variation in SLC11A1 expression by binding directly to the microsatellite during macrophage activation
- Finds no association between NRAMP1 polymorphism and increased susceptibility to multibacillary leprosy.
- Our findings suggest that NRAMP1 is a plausible candidate gene for systemic sclerosis.
- reduced NRAMP1 function in the common genetic variant shown to be associated with tuberculosis susceptibility in pediatric patients
- data suggested that genetic variations in SLC11A1 may affect the incidence of MDR-TB and clinical features of pulmonary tuberculosis
- Suggestive linkage was found at the SLC11A1 locus for the quantitative Mitsuda reaction in 19 Vietnamese families with leprosy.
- SLC11A1 genes may change the balance between elastase produced by leukocytes during phagocytosis
- Genetic polymorphisms of NRAMP1 might be associated with susceptibility to Crohn's disease.
- This study hypothesizes that Nramp1 may participate in the recycling of iron acquired through phagocytosis
- our work suggests that allele 3 at the functional (GT)n repeat polymorphism of NRAMP1 is associated with susceptibility to sarcoidosis in the Greek population
- Findings suggest that the SLC11A1 polymorphisms could be used as markers for genetic susceptibility to chronic obstructive pulmonary disease.
- Subjects homozygous for allele 2 at a repeat element in the promoter region of SLC11A1 had increased gene expression.
- Cis element binding sites for Sp1 and C/EBP factors regulating the expression of the NRAMP1 gene in myeloid cells.
- Results suggest that allelic variation in the NRAMP1 promoter may contribute to multiple sclerosis susceptibility in the Sardinian population.
- Variant genotypes at the 3'UTR locus in the SLC11A1 gene were associated with pediatric tuberculosis in Chinese patients.