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Validated All-in-One™ qPCR Primer for CERK(NM_022766.5) Search again
Product ID:
HQP017152
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
LK4, dA59H18.2, dA59H18.3, hCERK
Gene Description:
ceramide kinase
Target Gene Accession:
NM_022766.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM].
Gene References into function
- Molecular cloning and functional characterization
- ceramide kinase and its product, C-1-P, have roles in arachidonic acid release and production of eicosanoids
- CERK translocates during activation from the cytosol to a lipid raft fraction
- Pleckstrin homology domain of CERK is not only indispensable for its activity but also act as a regulator of CERK activity.
- sphingoid chain was also required for substrate recognition by CERK
- Recombinant CERK was analyzed with regard to dependence on divalent cations, to substrate delivery, specificity, and stereoselectivity. CERK associated with the plasma membrane in CHO cells, which is mediated by the N-terminal putative pleckstrin domain.
- CERK-pleckstrin homology domain plays a crucial role in plasma membrane targeting through its binding to phosphatidylinositol 4,5-bisphosphate and subsequent induction of ceramide-1-phosphate production in the vicinity of the membrane.
- These results suggest that ceramide kinase determines the balance between pro-apoptotic ceramide and anti-apoptotic C1P to regulate cell fate, reminiscent of its function in plants.
- Results demonstrate that CERK localizes to areas of eicosanoid synthesis and uses a ceramide "pool" transported in an active manner via CERT.
- HL-60 cells are useful for studying CerK functions in leukocyte differentiation, and they also suggest that CerK might have an important role in such differentiation.
- higher Ca2+ signal observed in hCERK transfected cells as well as the fact that CERK colocalized with EIgG during phagocytosis support hypothesis that Ca2+ signaling is an important factor for increasing phagocytosis and is regulated by CERK
- identification of a cluster of cysteines, C(347)XXXC(351)XXC(354), essential for CerK function