|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for SELP(NM_003005.3) Search again
Product ID:
HQP016746
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL
Gene Description:
selectin P
Target Gene Accession:
NM_003005.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq].
Gene References into function
- changes in the CD62 expression induced by a drug, namely clopidogrel, or by a disease, namely diabetes, are paralleled by changes in PDGF secretion
- Heparin blocks P-selectin-mediated interactions of endogenous platelets with sialylated fucosylated mucins on circulating carcinoma cells and this reduces tumor cell survival.
- results showed that sP-selectin and sE-selectin levels were higher in patients with lung cancer compared to normal donors; increased levels of sP-selectin and sE-selectin were associated with squamous lung cancer at late stages but not adenocarcinoma
- Eosinophil interaction with endothelial P-selectin is far more important than interaction with E-selectin for recruitment into the inflamed skin of patients with atopic dermatitis.
- Hypercholesterolemia primes platelets for recruitment via VWF, GPIb alpha, and P-selectin to lesion-prone sites, before lesions are detectable.
- Attachment of the PSGL-1 cytoplasmic domain to the actin cytoskeleton is essential for leukocyte rolling on P-selectin.
- Specific haplotypes of the P-selectin gene are associated with myocardial infarction.
- Conjunctival biopsies excised from patients following cataract surgery provided circumstantial evidence that endothelial P-selectin might play a role in the surgery-induced up-regulation of leukocyte rolling.
- thrombin stimulated rapid expression of P-selectin on the surface of endothelial cells was accompanied by qualitatively parallel increases in reactive oxygen species generation
- levels in children with acute and chronic idiopathic thrombocytopenic purpura and its relationship with mega-dose methylprednisolone therapy
- Role of P-selectin in the adhesion of platelets to intestinal venules during ischemia/reperfusion of the intestine in mice.
- tested hypothesis that patients with systemic sclerosis would have raised levels of soluble SELP and that there would be a relationship with autoantibodies
- The novel varepsilon and eta and atypical zeta, but not the conventional alpha and beta and the novel delta PKCs, may be involved in the signaling pathways involved in thrombin-induced human platelet P-selectin expression
- A novel dual role for P-selectin is revealed in its enhancement of the generation of CD14+CD16+ dendritic-like cells but inhibition of macrophage differentiation from human peripheral blood monocytes.
- Data show that chimeras of P-selectin and immunoglobulin (P-sel-Ig) induced formation of procoagulant microparticles in human blood through P-selectin glycoprotein ligand-1.
- Platelet P-selectin is independently associated with atherosclerotic arterial wall changes in human subjects
- The P-selectin cytoplasmic domain directs the cellular storage of a recombinant chimeric factor IX.
- P-selectin bound to heparin with dissociation constant, k(D), of 115 +/- 6 nM. The very slow k(off) and the reduced k(on), but apparently not the K(d), are responsible for adhesion, but not rolling of A375 cells, to P-selectin under flow.
- P-selectin elevation in chronic liver disease may suggest a possible pathogenetic role in the course of liver cirrhosis.
- The formation of monocyte clusters is mediated by platelet-expressed P-selectin and monocyte-expressed PSGL-1.
- P-selectin may anchor ultralarge von willebrand factor strings to endothelial cells and facilitate their cleavage by ADAMTS13.
- Thrombin decreased the recruitment of P-selectin into clathrin-coated pits, slowed the internalization of P-selectin, and reduced neutrophil rolling on P-selectin.
- conclusion, NMSO3 acts as a specific inhibitor for P-selectin-mediated cell adhesion and for adhesion-dependent leukocyte activation.
- Selectin P gene polymorphisms is associated with incident stroke
- maximal P-selectin translocation and subsequent neutrophil adhesion was mediated by VEGF-A(165) on the activation of VEGFR-2/NRP-1 complex and required PAF synthesis.
- P-selectin rolling involves core 2 beta1-6-N-glucosaminyltransferase and dimerization of P-selectin glycoprotein ligand-1
- platelet P-selectin and microparticle PSGL-1 have roles in thrombus formation [review]
- the adhesion receptor P-selectin has a role in hemostasis [review]
- P-selectin may play an important inflammation-related role in plaque development.
- Results suggest that the 6-O-sulfate group of glucosamine units in heparin is critical for the inhibition of P-selectin-mediated tumor cell adhesion.
- platelet-activating factor or interleukin 8 acted in concert with P-selectin for further enhancing the activation of alphaMbeta2
- P-selectin might represent a prognostic indicator in the management of patients with colorectal canceer.
- Freshly sorted and in vitro expanded CD62L(-) memory T cells were less responsive to allogeneic antigen stimulation than were CD62L(+) naive T cells
- selectin-dependent inflammation-seeking properties are imprinted by epigenetic modifications upon T-cell differentiation into effector cells.
- the orientation and length of P-selectin, E-selectin, or CD16A receptor influences its rate of encountering and binding a surface ligand but does not subsequently affect the stability of binding
- Neither liver transplantation nor liver resection influences GPIIb/IIIa and P-selectin expression on circulating platelets.
- a sustained rise in [Ca 2+ ]i due to an increase in the frequency and amplitude of transient calcium spiking in single platelets was dependent upon engagement of both P-selectin and FcgammaRIIA
- neutrophils stabilize selectin-mediated rolling by rapidly adjusting tether number in response to changes in wall shear stress
- in whites and South Asians the C allele of the Thr715Pro P-selectin polymorphism is associated with lower sP-selectin levels. Lower levels of sP-selectin were not accounted for by this polymorphism in blacks, in whom the C allele was very rare.
- levels of soluble and platelet P-selectin are increased in acute ischemic stroke, indicating a possible role of P-selectin
- P-selectin, either soluble or in its membrane-bound form, induces phosphatidylserine exposure in monocytes through a mechanism dependent on PSGL-1
- The levels of P-selectin, tPA antigen, and PAI-1 activity were all significantly higher in stroke patients compared with controls.
- The 290Asn (S290N) variant of P-selectin was associated with a higher prevalence and greater degree of albuminuria in European American siblings of type 2 diabetic families.
- biophysical analysis of PSGL-1/P-selectin neutrophil adhesion
- The P-selectin Thr715Pro polymorphism is not associated with incident CHD or ischemic stroke in either whites or African-Americans.
- Compared with those treated with bivalirudin, patients treated with UFH+E exhibited a 45% decrease in the capacity of platelets to bind fibrinogen.
- Thrombin-mediated endothelial-cell release of von Willebrand factor (VWF) and P-selectin functionally links protease-activated receptors (PARs) to thrombosis and inflammation.
- variant isoforms of CD44 on LS174T colon carcinoma cells possess selectin binding activity, in contrast to the standard isoform of CD44 on hematopoietic-progenitor cells.
- P-selectin participates with GPIIb-IIIa in the initiation of platelet aggregation
- P-selectin expression, but not activated GPIIb/IIIa, is enhanced in ADP-activated platelets in the inflammatory stage of Takayasu's arteritis. SELP may play a significant role in the inflammatory and thrombotic responses associated with intractable TA
- Here we provide a comprehensive summary of the functional roles and the biological importance of P-selectin-mediated cell adhesive interactions in the pathogeneses of inflammation, thrombosis, and the growth and metastasis of cancers.
- P-selectin is recruited to forming Weibel-Palade bodies in the trans-Golgi by AP-3-independent mechanisms that use sorting information within both the cytoplasmic tail and the lumenal domain of the receptor
- Platelet activation and platelet CD62 may participate in the accelerated target organ injury observed in high risk patients with severe hypertension
- Sympathetic overactivity and reduced heart rate variability are important determinants for left atrium spontaneous echo contrast (LASEC) formation and increased s-P selectin levels.
- Activation of STAT6 appears to be a key factor in P-selectin expression induced by substance P and IL-4 because treatment with STAT6 decoy oligodeoxynucleotides significantly inhibited P-selectin expression.
- Both circulating PDMPs and P-selectin expression were enhanced in association with stent-induced platelet activation; however, the time course of changes in these two platelet activation markers was different
- Specificity analysis reveals that RR2r3s4-1 does not cross react with murine PSGL-1 and thus requires more than tyrosine sulfate for binding to human PSGL-1
- vascular cell adhesion molecules (sICAM-1 and sVCAM-1) and also P-selectin were higher in patients than healthy controls
- The periprocedural evaluation of sP-selectin and sE-selectin in peripheral venous blood in patients undergoing elective coronary stenting provides no prognostic information in terms of clinical restenosis prediction.
- The endothelial reperfusion injury after liver transplantation does not show an increase in p-selectin.
- positive correlation of CD62P with erythrocyte sedimentation rate and C-reactive protein in rheumatoid arthritis
- Specific P-selectin and P-selectin glycoprotein ligand-1 genotypes/haplotypes may have roles in coronary heart disease and ischemic stroke
- lysophosphatidylcholine dose-dependently induced expression of vascular cell adhesion molecule-1 and P-selectin, accompanied by the activation of transcription factor nuclear factor kappaB.
- Patients with Reynaud's Phenomenon (RP) secondary to Systemic Sclerosis SSc) had a higher expression of CD62P than patients with primary RP or healthy controls.
- P-selectin-dependent functions of platelets and other cells play a role in myocardial infarction.
- P-selectin levels were positively correlated with VCAM-1 and HbA1c levels, indicating that increased level of oxidative stress marker, proinflammatory markers, and their downstream effectors adhesion molecules occur in type 2 DM.
- results suggest a contribution of P-selectin and CRP genes in modulating susceptibility to cognitive decline after cardiac surgery
- The increased values of VEGF, PLA2-LDL and P-selectin in patients with long standing pulmonary hypertension are related to severe endothelial dysfunction and may have prognostic values.
- Increased sP-selectin concentrations are associated with VTE and genotype status. sP-selectin concentrations are lower in individuals carrying the P-selectin Pro715 variant than in those without this variant.
- CRH, PLAC1, and selectin-P are distributed differently in preeclampsia cases compared to controls and correlate with signs of preeclampsia.
- ADAM28s promotes PSGL-1/P-selectin-mediated leukocyte rolling adhesion to endothelial cells and subsequent infiltration into tissue spaces through interaction with PSGL-1
- in type 2 DM individuals, the significantly elevated sP-selectin levels are not due to the Thr715Pro P-selectin gene polymorphism
- Plasma levels of P-selectin are determined by platelet turn-over and the P-selectin Thr715Pro polymorphism.
- Patients with isolated coronary artery ectasia (CAE) have raised levels of plasma P-selectin, beta-TG and PF4 compared with control participants with angiographically normal coronary arteries, suggesting increased platelet activation in patients with CAE
- In women with subsequent preeclampsia, sL-selectin and sVCAM-1 concentrations were significantly lower, whereas sE-selectin, sP-selectin and sICAM-1 levels were significantly higher, compared with controls at mid-pregnancy (p<0.05).
- CD36, ICAM-1, and P-selectin are major contributors to the dynamic process of Plasmodium falciparum-infected red blood cell adhesion by different mechanisms in vivo
- TLR-4 gene polymorphisms are associated with lower levels of P-selectin and von Willebrand factor among hypercholesterolemic patients.
- A new method for determining receptor-ligand association/dissociation events across the interface of two surfaces (two-dimensional binding) by monitoring abrupt decrease/resumption in thermal fluctuations of a biomembrane force probe.
- P-selectin Thr715Pro polymorphism is not associated with the risk of severe preeclampsia.
- One SNP, threonine-to-proline substitution at position 715, in SELP explained significant variation in circulating P-selectin concentrations, even after accounting for known clinical correlates.
- CD62P expression and the number of aggregates are increased after exercise.
- Platelet P-selectin expression increased during the common cold.
- SELP and IRAK1 were identified as novel SLE-associated genes with a high degree of significance, suggesting new directions in understanding the pathogenesis of systemic lupus erythematosus.
- associations between negative affect (i.e., depressive and anxious symptoms) with increased norepinephrine and P-selectin responses to an acute psychological stress
- The correlation between soluble P-selectin and VEGF in sickle cell disease and HbSC disease is consistent with the view that VEGF is released from platelets during in vivo activation.
- Data show that P-selectin, but not L- or E-selectin, concentrations are significantly increased in male and female pseudoxanthoma elasticum patients.
- soluble E-selectin and soluble P-selectin in blood plasma of labouring women do not appear to have a role in preeclampsia
- increased C reactive protein (CRP) levels in systemic scleroderma correlated with P selectin and Platelet Glycoprotein GPIIbIIIa complex expression
- P-selectin-PSGL-1-induces TF and IL8 expression through Lyn phosphorylation, and part of the inhibitory effect of IL10 depends on reduced phosphorylation
- analysis of P-selectin polymorphisms' influences on P-selectin serum concentrations and on their familial correlation
- Increased VCAM-1 and P-selectin expression was associated with tricuspid regurgitant jet elevation regardless of etiology
- SELP haplotypes modulate plasma concentrations of sP-selectin and affect the risk of recurrent venous thromboembolism.
- Our data suggest that in the absence of overt activation, PSGL-1 (P selectin ligand 1-P-selectin-dependent platelet binding to monocytes represents a normal physiological process with little impact on the potential of monocytes to cause vascular injury
- Common p selectin polymorphisms were associated with soluble P-selectin and carotid intima media thickness in young adults, but the patterns of association differed between Caucasian americans and African Americans
- High sP-selectin plasma levels independently predict venous thromboembolism in cancer patients.
- while platelet microparticles were higher, CD62P (selectin P) levels were not significantly higher in the women with recurrent spontaneous abortion compared with the control group
- P-selectin is present in placenta and gestational myometrium; its concentration is respectively 30- and 25-times higher than in plasma
- Suggest that P-selectin levels could be useful in the early assessment of the severity of acute pancreatitis.
- Report maspin, E-selectin, and P-selectin expressions in papillary thyroid carcinomas and their correlation with prognostic parameters.
- Induction of hypercortisolism in healthy volunteers was not associated with changes in the platelet activation marker P-selectin or the number of circulating platelet-leucocyte aggregates.
- Atorvastatin induces associated reductions in platelet P-selectin, oxidized low-density lipoprotein, and interleukin-6 in patients with coronary artery diseases.
- the role of PSLG1 decamers is to extend PSGL-1 N terminus far above the cell surface to support and stabilize leukocyte rolling on L- or P-selectin
- raised plasma sP-selectin may influence the early progression of vascular disease by promoting leukocyte adhesion to the endothelium in PAOD, through Mac-1-mediated rolling and dependent on endothelial P-selectin expression.
- Platelet surface P-selectin expression is highly correlated with serotonin transporter density in human subjects.
- These observations demonstrate that Numb3 is an endocytic receptor for P-selectin and may be responsible for the rapid internalization of P-selectin when endothelial activation ends.