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Validated All-in-One™ qPCR Primer for SELE(NM_000450.1) Search again
Product ID:
HQP016744
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD62E, ELAM, ELAM1, ESEL, LECAM2, selectin-e
Gene Description:
selectin E
Target Gene Accession:
NM_000450.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq].
Gene References into function
- circadian variations in healthy adults and in subjects with type II diabetes; possible role in increased vascular wall uptake of lipoproteins in diabetic subjects
- The e-selectin C allele was more common in systemic lupus erythematosus than in controls. E-selectin may be a susceptibility gene to systemic lupus erythematosus in these populations.
- Premature labor is associated with up-regulation of adhesion molecules in the lower uterine segment
- activation of expression by HIV-1 tat protein via NF-kappa B-dependent mechanism
- two SNPs in the E-selectin gene and six SNPs in the L-selectin gene were significantly associated with IgAN in Japanese patients
- significant increase in plasma levels between day 1 and day 5 of G-CSF-induced stem cell mobilization; possible effect on leukocyte-endothelial cell interactions
- role of phosphoserine-type di-leucine motif in E-selectin internalization
- E-selectin and vWF were not correlated with percent body fat, but were negatively correlated with M (r= -0.65 and -0.46, both P<0.001) and positively correlated with CRP (r=0.46, and 0.33, both P<0.05).
- Human mast cell progenitors use alpha4-integrin, VCAM-1, and PSGL-1, E-selectin for adhesive interactions with human vascular endothelium under flow conditions
- relation between glycemic control, hyperinsulinism and plasma concentration in patints with glucose intolerance or NIDDM
- results showed that sP-selectin and sE-selectin levels were higher in patients with lung cancer compared to normal donors; increased levels of sP-selectin and sE-selectin were associated with squamous lung cancer at late stages but not adenocarcinoma
- inhibition of cytokine induction of promoter by 17beta-estradiol
- Eosinophil interaction with endothelial P-selectin is far more important than interaction with E-selectin for recruitment into the inflamed skin of patients with atopic dermatitis.
- E-selectin blood levels are no different in non-Hodgkin's lymphoma patients and controls
- X-irradiation 2-5 h before stimulation decreased the characteristic transient expression of E-selectin after TNFalpha stimulation.
- the preferential expression of selectin E on the endothelium of femoral and iliac arteries in thromboangiitis obliterans
- CD62L on human cultured T lymphoblasts is one of several glycoproteins that interacts directly with E-selectin and contributes to rolling under flow.
- E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular adhesion molecule-1 (VCAM-1) were measured in serum from hypertensive patients with LV hypertrophy
- Downregulation of e-selectin is associated with nodal metastasis in breast cancer
- circulating soluble selectin E in acute myeloid leukemia is correlated with peripheral blast cell counts, extramedullaary infiltration, relapse and mortality
- Conjunctival biopsies excised from patients following cataract surgery did not show that endothelial E-selectin might play a role in the surgery-induced up-regulation of leukocyte rolling.
- enhanced expression of E-selectin may contribute to the development of diabetic maculopathy in type 2 diabetes
- A serine to arginine (S128R) polymorphism in E-selectin extends the range of lymphocytes recruited by E-selectin, which may provide a mechanistic link between this polymorphism and vascular inflammatory disease.
- interacts with fuco-oligosaccharides of the blood group family
- is a potent mediator of human dermal microvascular endothelial cell (HMVEC) chemotaxis, which is predominantly mediated through the Src and the phosphatidylinositiol 3-kinase (PI3K) pathways
- The E-selectin Ser128Arg polymorphism can functionally alter leukocyte-endothelial interactions as well as biochemical and biological consequences, which may account for the pathogenesis of myocardial infarction.
- Endothelial cells have increased expression of VCAM-1, E-selectin, and ICAM-1 when exposed to sickle blood cells.
- Enhanced expression of E-selectin on the vascular endothelium of peripheral nerve in critically ill patients with neuromuscular disorders.
- interacts with Helicobacter pylori isolates from patients with chronic gastritis, duodenal ulcer and gastric cancer
- Extravasated and oxidized LDL in xanthoma adds to foam cell recruitment by activating tyrosine kinase and inducing adhesion of monocytes to dermal microvascular endothelial cells through VCAM-1 and E-selectin.
- High serum soluble E-selectin levels are associated with postoperative haematogenic recurrence in esophageal squamous cell carcinoma
- The adhesion of E-selectin expressing CHO cells to the coated mucins was analyzed in a flow system revealing that the MUC1 mucin adhered better than the CD43 mucin
- central obese subjects with concomitant higher levels of selectin E and ACE DD genotype may be characterized by early cardiovascular alterations and then considered a particular subset of subjects at higher risk of cardiovascular disease
- Blood levels do not differ in normal and coronary artery disease patients.
- Localization of E-selectin in lipid rafts is necessary for its association with, and activation of, phospholipase C gamma.
- Adrenomedullin and ACTH induce cell surface expression of adhesion molecules E-selectin, VCAM-1, and ICAM-1 on human umbilical vein endothelial cells.
- E-selectin has a role in promoting growth inhibition and apoptosis of human and murine hematopoietic progenitor cells independent of PSGL-1
- E-selectin and its ligand-sLeX are closely correlated with the metastasis of hepatocellular carcinoma
- minor trauma to skin may induce expression of E-selectin, ICAM-1 and IL-8
- Coexpression of E-selectin with ICAM-1 and CCL17 on blood vessels in noninflamed skin provides the basis for a model of cutaneous immunosurveillance system active in the absence of pathologic inflammation.
- Data indicate that weight loss is more important than glycaemic control in regulating circulating levels of E-selectin in morbidly obese subjects.
- even a short-term exposure of endothelial cells (ECs) to high glucose concentration leads to their activation associated with increased expression of adhesion molecules such as ELAM-1, VCAM-1 and ICAM-1.
- E-selectin has a role in tumor cell adhesion but its expression is inhibited by lovastatin and all-trans retinoic acid
- Expresson is reduced in treatment with methotrexate in bullous pemphigoid.
- These results suggest a BMI-specific effect of L/F554 polymorphism of the E-selectin gene on blood pressure, and strengthen the hypothesis that E-selectin is implicated in hypertension.
- E-selectin may play an important inflammation-related role in plaque development.
- The C allele at the +561 position of the E-selectin gene is associated with significantly reduced risk of developing sarcoidosis in patients with erythema nodosum.
- The severity and diversity of the histopathology of lupus nephritis are partially associated with the expression of E-selectin, P-selectin, and VCAM-1 in glomeruli.
- E-selectin is required for the antiangiogenic activity of endostatin in vivo and ex vivo and confers endostatin sensitivity to nonresponsive human endothelial cells in vitro
- IVIG inhibits NF-kappaB activation induced by TNF-alpha in cultured endothelial cells of coronary arteries, thereby possibly modulating IL-6 production and E-selectin expression
- Our study suggests that the E-selectin polymorphism may be associated with severity of atherosclerotic disease, but does not allow us to conclude that it is actually a risk factor for atherosclerosis.
- E-selectin is unique among selectins in its capacity for clustering sialylated ligands and transducing signals leading to neutrophil arrest in shear flow.
- sE-selectin may have a role in progression and metastasis of colorectal neoplasms
- the orientation and length of P-selectin, E-selectin, or CD16A receptor influences its rate of encountering and binding a surface ligand but does not subsequently affect the stability of binding
- ICAM-1, VCAM-1, E-selectin and interleukin-6 expression are enhanced by glucose and regulated by poly(ADP-ribose) polymerase in human umbilical endothelial cells
- Clustered monocyte cell adhesion correlated with endothelial cells coexpressing intercellular adhesion molecule-1 (ICAM-1) and E-selectin as result of a flow-induced, selective upregulation of E-selectin expression in a subset of ICAM-1-expressing cells.
- role of E-selectin in activating integrins to modulate adhesion and regulate integrin-mediated events
- CLA and E-selectin are bound by T cells and have roles in skin inflammation
- Increases in serum E-selectin is associated with rheumatoid arthritis
- Cell expression in response to tobacco smoke extract.
- In myeloperoxidase-ANCA-positive microscopic polyangiitis, higher sICAM-1 and sELAM-1 levels during active phase and their slower decline during treatment period could be prognostic risk factor for chronic renal failure.
- Direct implication of adhesion molecules in the pathogenesis or progression of type 2 diabetic retinopathy cannot be supported.
- concluded that in conjunction with serum triglycerides and serum leptin, serum E-selectin is another important independent factor associated with insulin resistance in nonobese Japanese type 2 diabetic patients
- Genomic rearrangement on SELE in arteriosclerosis was studied.
- conclude that the E-selectin variants examined are not important genetic risk factors for type 2 diabetes in women
- FBI-1 enhances transcription of the NF-kappaB-responsive E-selectin gene by nuclear localization of the p65 subunit of NF-kappaB
- SR genotype of the E-selectin gene polymorphism in codon 128 is a genetic factor that may determine an individual's susceptibility for CAD in Chinese.
- CD43 is a T-cell E-selectin ligand distinct from PSGL-1 which expands the role of CD43 in the regulation of T-cell trafficking.
- Tethering by E-selectin triggers b2-integrin-dependent adhesion and a b2-integrin-dependent outside-in signal resulting in tyrosine phosphorylation of a 110 kDa protein, in human PMN.
- Hepatocyte growth factor ameliorates acute renal inflammation in part by downregulating E-selectin mediated macrophage adhesion to the inflamed endothelium.
- in end-stage renal disease, the Leu554Phe polymorphism of E-selectin gene is associated with the severity of carotid atherosclerosis
- expression of HCELL confers robust and predominant tumor cell binding to E- and L-selectin
- The mutant 128R allele of the E-selectin gene is associated with angiographic severe coronary artery disease (CAD) in Saudi Arabs. This association is lost after adjustment for traditional CAD risk factors.
- Serum E-selectin, ICAM-1 and integrin beta1 expression levels are probably related to the metastasis and relapse of gastric cancer.
- Our results show that E-selectin gene polymorphisms represent an increased risk for ischemic CVD in the Japanese population without diabetes mellitus and hypercholesterolemia.
- E-selectin immunoadhesin decreases leukocyte attachment and rolling in vitro.
- Homozygosity for the S128R E-selectin allele appears to increase the risk for recurrent venous thromboembolism several fold.
- A significant increase in allele frequencies of 128R of E-selectin and the associated genotype SR was observed in celiac disease patients.
- Tibolone causes a rapid and sustained decrease in circulating levels of VCAMs; the effect is lost soon after stopping the treatment.
- The interval was significantly longer in IgA nephropathy patients with a homoallele of C in C1402T, C1402/C1402, of the E-selectin gene, or a homoallele of C in C712T, C712/C712, of the L-selectin gene compared to others.
- G-CSF has a role in the induction of E-selectin ligands on myeloid cells, thus providing mechanistic insight into the pathobiology of G-CSF complications
- The sE-selectin, rheumatoid factor, and erythrocyte sedimentation rate, in addition to clinical indices, were associated with clinical activity in rheumatoid arthritis.
- We were not able to demonstrate an association between the Hemicentin-1, hOgg1, and E-selectin SNPs and age-related macular degeneration development in the currently available cases and controls.
- HOSCN induces E-selectin, ICAM-1, and VCAM-1 expression in HUVEC; HOSCN up-regulation of these adhesion molecules is transcriptionally mediated through a mechanism dependent upon activation of NFKB and constitutively suppressed by PI3K-Akt pathway.
- The periprocedural evaluation of sP-selectin and sE-selectin in peripheral venous blood in patients undergoing elective coronary stenting provides no prognostic information in terms of clinical restenosis prediction.
- Increased plasma levels of E-selectin is associated with advanced stage of breast cancer
- These findings suggest that the E-selectin S128R polymorphism can functionally affect tumor-endothelial interactions as well as motility and signaling properties of neoplastic cells that may modulate the metastatic phenotype.
- higher levels of E-selectin and ICAM-1 were consistently associated with increased diabetes risk in a multiethnic cohort of U.S. postmenopausal women, implicating an etiological role of endothelial dysfunction in the pathogenesis of type 2 diabetes.
- These results suggest that the Arg/Arg genotype of the E-selectin gene polymorphism in codon 128 is a genetic factor that may determine an individual's susceptibility for brucella infection.
- HOXA9 participates in the transcriptional activation of SELE in endothelial cells.
- Data show that in bullous pemphigoid, expression of E selectins was detected mainly in basal keratinocytes and in inflammatory infiltrates in the dermis.
- the risk of acquiring coronary artery disease in patients with type 2 diabetes mellitus increases significantly in the presence of the 128R mutant allele of the E-selectin gene
- study demonstrates that the functional counter-receptors for E-selectin at the cell surface of Colo201 human colon cancer cells are localized in detergent-insoluble membrane microdomains (DIM).
- In women with subsequent preeclampsia, sL-selectin and sVCAM-1 concentrations were significantly lower, whereas sE-selectin, sP-selectin and sICAM-1 levels were significantly higher, compared with controls at mid-pregnancy (p<0.05).
- OPG but not OPN stimulated a dose-dependent increase in the expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and E-selectin by endothelial cells in the presence of TNF-alpha
- data are in keeping with the hypothesis that in systemic inflammation altered E-selectin shedding may play a role in arterial damage & implicates this adhesion molecule in atherosclerotic complications in a high-risk condition like end-stage renal diseas
- E-selectin Serl28Arg polymorphism is not associated with the risk of severe preeclampsia.
- This is the first report of a leptospiral protein capable of up-regulating the expression of endothelial cell adhesion molecules ICAM-1 and E-selectin.
- Increased blood levels in rheumatoid arthritis are diminished after treatment with methotrexate.
- Acetylcholine-induced vasodilation in the forearm, but not flow mediated vasodilation, was inversely related to CRP and e-selectin levels independently of traditional risk factors in elderly subjects
- Endothelial dysfunction exists in atrial fibrillation and is related to dysfuncion of E selectin levels.
- E-selectin clusters in both clathrin-coated pits and lipid rafts of endothelial cells but is internalized in clathrin-coated pits.
- A significant association of E-selectin and susceptibility to Graves' disease is found in a Chinese population.
- Data show that P-selectin, but not L- or E-selectin, concentrations are significantly increased in male and female pseudoxanthoma elasticum patients.
- Mean sICAM-1 value was higher in patients with active giant cell arteritis than in cluster headache patients during the active cluster period.
- Xenon, isoflurane, nitrous oxide, and morphine do not prevent TNF-alpha induced E-selectin expression.
- soluble E-selectin and soluble P-selectin in blood plasma of labouring women do not appear to have a role in preeclampsia
- CEA and CD44v cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin at elevated shear stresses.
- Higher serum concentrations of endothelin-1 (ET-1), E-selectin, and Thrombomodulin were observed in SLE patients in comparison with controls
- Suggest that E-selectin levels could be useful in the early assessment of the severity of acute pancreatitis.
- Oxidized LDL impairs angiogenic properties of endothelial progenitor cells at sub-apoptotic levels by downregulation of E-selectin and integrin alphavbeta5, both substantial mediators of EPC-endothelial cell interaction.
- E-selectin regulates diapedesis of circulating cancer cells.
- Patients with inactive TA have elevated levels of sE-selectin, sVCAM-1, and sICAM-1 that might indicate persistent vasculopathy in clinically inactive disease.
- Report maspin, E-selectin, and P-selectin expressions in papillary thyroid carcinomas and their correlation with prognostic parameters.
- NF-kappaB, but not p38, is critical for TNF-alpha-induced expression of ICAM-1, VCAM-1 and E-selectin
- Study points to synergistic interactions between 561A > C or 98G > T polymorphisms of E-selectin gene and hypercholesterolemia that cause a significant increase in the susceptibility to coronary artery disease.
- Levels of E-selectin positively correlated with high triglyceride levels in type 2 diabetic subjects with silent ischemia.
- VEGF, sVCAM-1, sICAM-1 and sE-selectin median levels were higher in hemodialysis patients compared to controls.
- Elevated serum sE-selectin was associated with a poor outcome of biliary atresia and probably plays a role in the physiopathology of liver injury in postoperative biliary atresia.
- Gb3 induces oxidative stress and up-regulates the expression of ICAM1, VACAM1 and selectin E in Fabry disease vascular endothelial cells.
- Angiostatin K1-3 induced E-selectin expression via AP1 and Ets-1 binding to the proximal E-selectin promoter (-356/+1), which was positively mediated by JNK activation.
- correlations found between sVCAM-1, sICAM-1 and sELAM-1 and the presence of retinopathy suggest that cellular adhesion and neovascularization may be linked processes
- Data suggest that squamous cell carcinomas evade the immune response at least in part by down-regulating vascular E-selectin and recruiting regulatory T cells.
- Changes in nailfold capillaroscopy, endothelial cell activation markers (VEGF, endothelin-1, E-selectin, and thrombomodulin) and features of systemic lupus erythematosus suggest microvascular abnormalities in the clinical manifestation of the disease.
- In recently diagnosed hypertensive subjects, hsCRP and sE-selectin levels are related to awake systolic blood pressure variability.