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Validated All-in-One™ qPCR Primer for SDC4(NM_002999.3) Search again
Product ID:
HQP016661
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
SYND4
Gene Description:
syndecan 4
Target Gene Accession:
NM_002999.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq].
Gene References into function
- Studies suggest that antithrombin regulates neutrophil migration via effects of its heparin-binding site on cell surface syndecan-4.
- Syndecan-4 mediates antithrombin-induced chemotaxis of human peripheral blood lymphocytes and monocytes
- Clustering induces redistribution of syndecan-4 core protein into raft membrane domains
- Syndecan-4 core protein mediates the effects of fibroblast growth factor (FGF)2 on cell function. PKCalpha activation and PDZ-mediated formation of a serine/threonine phosphatase-containing complex by syndecan-4 are downstream events of FGF2 signaling.
- SDC4 regulates inositol phospholipid binding and signaling
- the focal adhesion component alpha-actinin interacts with syndecan-4 in a beta-integrin-independent manner
- Syndecan-4 can promote cell spreading in a beta(1) integrin-dependent fashion through PKCalpha and RhoA
- endotoxin-induced adhesion of leukocytes to endothelium can be reversed by ligation of syndecan-4 with antithrombin's heparin-binding site
- syndecan-4/CXCR4 complex is likely a functional unit involved in SDF-1 binding
- human trabecular meshwork cells express only syndecan-1, and not syndecan-4, at the cell surface, and focal adhesion and stress fiber formation occur independent of syndecan-4
- Syndecan-4 has a critical role in thrombin-induced migration and proliferation in human vascular smooth muscle cells
- CXCL12 directly binds to syndecan-4 in a glycosaminoglycan-dependent manner
- may play important roles in the regulation of inflammatory effects of platelets
- Plasmin and thrombin accelerate shedding of syndecan-4 ectodomain, which generates cleavage sites at Lys(114)-Arg(115) and Lys(129)-Val(130) bonds
- the transmembrane domains are sufficient for inducing dimerization and transmembrane domain-induced oligomerization is crucial for syndecan-2 and syndecan-4 functions
- The H. pylori- and TLR-induced increase in syndecan-4 mRNA was blocked by the proteosome inhibitor MG-132 suggesting a role for nuclear factor kappaB (NF-kappaB) in the regulation of syndecan-4 gene expression
- Syndecan-1 and syndecan-4 may have roles in progression of breast carcinoma
- Stress-induced effects on smooth muscle cell syndecan-4 expression and shedding may represent an additional component of proinflammatory, growth-stimulating pathways activated in response to changes in the mechanical microenvironment of the vascular wall
- An alternate pathway mediated by the extracellular domains of syndecans-2 and -4 is characterized that is independent of both heparan sulphate and syndecan signaling.
- These results identify syndecan-4 as a novel receptor for the N-terminus of TSP-1 and suggest that TSP-1 N-terminal pro-angiogenic activity is linked to its capacity of interfering with syndecan-4 functions in the course of cell adhesion.
- analysis of how a peptide derived from tenascin-C induces beta1 integrin activation through syndecan-4
- syndecan TMD homodimerization and heterodimerization can be mediated by GxxxG motifs and modulated by sequence context
- syndecan-4 is a critical intrinsic regulator of inflammatory reactions via its effects on OPN function
- This study provides evidence for the up-regulation of syndecan-2 and -4 in human primary CD4 T cells during in vitro activation and suggest an inhibitory role for these syndecans in CD4 T cells.
- TG-FN binding to syndecan-4 activates PKCalpha leading to its association with beta(1) integrin, reinforcement of actin-stress fiber organization, and MAPK pathway activation
- None of the SDC4 polymorphisms showed a difference in their allelic distribution between leg ulcer patients and controls. SDC4 may play a role in wound healing, but expression abnormalities in uninvolved dermis may contribute to venous ulcer development.