|
ORF cDNA clones
|
CRISPR / TALEN
|
Lentivirus
|
AAV
|
TALE-TF
|
ORF knockin clones
|
|
Antibody
|
Proteins
|
miRNA target clones
|
qPCR primers
|
shRNA clones
|
miRNA products
|
Promoter clones
|
Validated All-in-One™ qPCR Primer for SDC1(NM_001006946.1) Search again
Product ID:
HQP016659
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD138, SDC, SYND1, syndecan
Gene Description:
syndecan 1
Target Gene Accession:
NM_001006946.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
|
|
| A | B |
|
Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
|
Summary
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins.
Gene References into function
- The detection of syndecan-1 in endometrial cancer of different clinical and histological stages could be of prognostic value in clinical diagnosis.
- The loss of syndecan-1 expression evident in acantholytic conditions
- mediates hepatocyte growth factor binding and promotes Met signaling in multiple myeloma
- Soluble syndecan-1 promotes growth and dissemination of transplanted human myeloma tumors in vivo in SCID mice implanted with human bones.
- review of structure, function, cell and tissue distribution
- regulation of expression in isoform specific manner by farnesoid X receptor
- Cells adherent via syndecan-1 do not spread, but can be induced to spread by Mn(2+), suggesting that activation of a beta(1) or beta(3) integrin partner is required.
- TNF-alpha and IL-1beta are capable of down-regulating syndecan-1 expression
- High syndecan-1 expression in breast carcinoma is related to an aggressive phenotype
- shedding of syndecan-1 promoted by MT1-MMP through the preferential cleavage of Gly245-Leu246 peptide bond stimulates cell migration
- The increased stromal syndecan-1 expression, coupled with its loss from the surface of carcinoma cells, may contribute to tumor cell invasion and the development of metastases
- results demonstrate that the laminin alpha3 LG4/5 modules within unprocessed laminin-5 permit its cell binding activity through heparan and chondroitin sulfate chains of syndecan-1
- the extracellular domain mediates an interaction that is necessary for dynamic cytoskeletal rearrangements whereas an interaction of the transmembrane domain is required for the initial spreading response
- results demonstrate that cell-surface syndecan-1 is a degradative target of heparanase (HPSE-1), and syndecan-1 regulates HPSE-1 biological activity
- serves as a primary human papillomavirus receptor protein in natural HPV11 infection of keratinocytes.
- MT4-MMP and the proteoglycan form of syndecan-1 have roles in ADAMTS-4 activation on the cell surface
- Growth-promoting loop exists between breast cancer cells and their stroma that depends on the activity of glycanated Sdc1.
- Consistent with a possible biochemical role for syndecan-1 in prostate cancer progression and metastasis, syndecan-1 expression correlated with serologic recurrence in Gleason sum 7 prostate cancer and was highly expressed in soft-tissue metastases.
- in hyperinflammatory lesions, ephrinB2 was predominantly expressed in macrophage-like cells and EphB4 in small venules; syntenin and syndecan-1 were up-regulated in EphB4-positive endothelial cells after stimulation with preclustered ephrinB2
- syndecan-1 and glypican-1 have roles in progression of ovarian cancer
- Altered matrix-dependent signaling due to increased levels of cell surface syndecan-1 may lead to epithelial cell invasion during early stages of tumorigenesis.
- Concomitant expression of syndecan-1 in both epithelium and stroma may be a predictor of unfavourable prognosis in breast cancer, and in contrast with previous studies, loss of epithelial syndecan-1 was associated with a more favourable prognosis
- syndecan-1 is likely to be a critical regulator of many cellular behaviors that depend on activated alpha(v)beta(3) integrins
- constitutive shedding corresponding to the basal level of soluble syndecan-1 ectodomain was significantly increased when cells were stimulated with P. gingivalis lipopolysaccharide
- osteoprotegerin affects monocyte mi-gration and protein kinase C and phosphatidylinositol 3-kinase/Akt activation via syndecan-1.
- syndecan-1 has a role in progression of invasive breast carcinomas through the remodeling of breast cancer tissue via interaction with other extracellular matrix components
- Expression of paxillin and syndecan-1 in hepatocellular carcinoma(HCC) may affect its invasive and metastatic ability. May be converse correlation between expression of paxillin and syndecan-1 protein in HCC.
- Stromal syndecan-1 expression is an independent prognostic marker in pancreatic cancer, whereas epithelial syndecan-1 expression predicts better prognosis only in resectable disease.
- Loss of syndecan-1 plays a role in the growth of G-type cancers of differentiated type gastric cancers at an early stage
- Low epithelial syndecan-1 expression was associated with a higher histological grade and a more advanced clinical stage of the colorectal cancer
- Malignant glioma cells express all types of syndecans. NF-kappaB participates in the upregulation of the syndecan-1 expression at the transcriptional level, and increased expression of syndecan-1 could associate with thrombospondin-1.
- stromal fibroblast-derived Sdc1 stimulates breast carcinoma growth and angiogenesis in vivo
- shed syndecan-1 or MMP7-syndecan-1 complexes may have roles in tumor progression
- Syndecan-1 and syndecan-4 may have roles in progression of breast carcinoma
- The ectodomain of the syndecan-1 core protein contains an active site that assembles into a complex with the alphavbeta5 integrin and regulates alphavbeta5 integrin activity.
- Certain immunomarkers like syndecan-1, kappa and lambda light chains and IgA heavy chain could be of much help in identifying early stage immunoproliferative small intestinal disease (IPSID).
- CD138 may have a role in progression of nodal diffuse large B cell lymphoma
- syndecan-1 may have a role in progression of B-cell chronic lymphocytic leukemia
- increase of Syndecan-1 in all tissue compartments and a redistribution from epithelium to stroma may be a characteristic feature for dense breast tissue
- syndecan-1 shedding may modulate the pathogenesis of specific microbes
- the endocytic path taken by Syn-1 is clathrin-independent and relies upon lipid raft function
- Lacritin targets the deglycanated core protein of SDC1 and not HS chains or SDC2 or SDC4. Binding requires partial or complete removal of HS chains by endogenous HPSE. This limits lacritin activity to HPSE release sites as an HPSE 'off/on switch'.
- Syndecan-1 expression is uniquely influenced by changes in the phase and magnitude of the local stress field.
- domain V of the gamma2 chain negatively regulates the integrin beta4 phosphorylation, probably through a syndecan-1-mediated signaling, leading to enhanced cell adhesion and suppressed cell motility
- Heparanase influences expression and shedding of syndecan-1
- In summary, ovarian carcinomas exhibit up-regulated expression of several extracellular matrix proteins, and syndecan 1 represents a novel tumor-associated marker in ovarian serous carcinomas.
- Decreased syndecan-1 is asociated with thyroid cancer
- upregulation of syndecan-1 may be a critical element for endometrial cancers in maintaining their viability
- SDC1 interaction with the LG4/5 domain in kalinin is essential for keratinocyte migration.
- syndecan-1 regulates keratinocyte proliferation differently during skin development and in healing wounds.
- Results indicate a lack of association between SDC-1 polymorphisms and risk of squamous cell carcinomas of the cervix.
- heparan sulfate and syndecan-1, the predominant intestinal epithelial heparan sulfate proteoglycan, are essential in maintaining intestinal epithelial barrier function.
- syndecan TMD homodimerization and heterodimerization can be mediated by GxxxG motifs and modulated by sequence context
- Syndecan-1 and beta1 integrin signaling downstream of laminin alpha1-derived peptide AG73 regulate adhesion and MMP production by human salivary gland tumor cell lines (CAC2 and M1).
- aberrant skin expression may be involved in the development of psoriasis
- n-3 polyunsaturated fatty acids (n-3 PUFA) upregulate SDC1 in breast cancer cellsbu activating PPARgamma, providing a chemopreventive effect.
- In vitro reconstructed normal human epidermis expresses differentiation-related proteoglycans (CD44, syndecan-1, desmosealin, and 7C1).
- syndecan 1 is up-regulated by n-3 fatty acids by a transcriptional pathway involving PPARgamma
- loss of syndecan-1 epithelial expression was of strong prognostic value in breast carcinomas
- Our findings thus suggest that a previously unknown link between integrin alpha2beta1 and syndecan-1 is important in regulating cell adhesion to collagen and in triggering integrin downstream signalling.
- Rab5 is a critical regulator of syndecan-1 shedding that serves as an on-off molecular switch through its alternation between the GDP-bound and GTP-bound forms.
- Membrane type 1 matrix metalloproteinase-mediated stromal syndecan-1 shedding stimulates breast carcinoma cell proliferation.
- syndecan-1 directly contributes to the growth and invasive ability of oral cancer cells
- Plasma levels of syndecan-1 increased significantly during coronary artery bypass grafting, with or without the use of cardiopulmonary bypass.