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Validated All-in-One™ qPCR Primer for CXCL11(NM_005409.4) Search again
Product ID:
HQP016649
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
H174, I-TAC, IP-9, IP9, SCYB11, SCYB9B, b-R1
Gene Description:
C-X-C motif chemokine ligand 11
Target Gene Accession:
NM_005409.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.
Gene References into function
- chemotactic activity for CXCR3-expressing cells; substrate for dipeptidylpeptidase IV
- I-TAC is a highly potent chemoattractant of normal blood CD4 and CD8 T cell transendothelial migration and a major mediator of blood memory T lymphocyte migration to inflammation.
- The IFN-gamma-inducible T cell alpha-chemoattractant was induced in human brain microvascular endothelial cells and astrocytes only after inflammatory stimuli.
- These data suggest that IFN-beta acts through PI3K to enhance the transactivation competence of NF-kappa B complexes through phosphorylation of p65 within the TAD of beta-R1.
- Levels of ITAC/CXCL11 were found elevated in patients with severe transplantation coronary artery disease (TCAD) compared with long-term survivors of transplantation without TCAD and healthy volunteers who had not undergone transplantation
- increased calpain activity in undifferentiated keratinocytes. inhibited calpain activity in fibroblasts. redifferentiated basal keratinocytes limit fibroblast repopulation of dermis of healed wounds while promoting re-epithelialization.
- A higher amount of I-TAC mRNA is expressed after exposure of keratinocytes to interferon-gamma, leading to migration of T cells from the dermis to the epidermis and representing a second step of chemotaxis following T cell recruitment from blood.
- CXCR3 ligands inhibit CCR3-mediated functional responses of both human eosinophils and CCR3 transfectants induced by all three eotaxins, with CXCL11 being the most efficacious antagonist.
- I-TAC, one of the most potent chemoattractants for activated T cells, is produced by hepatocytes in the HCV-infected liver and plays an important role in T cell recruitment and ultimately the pathogenesis of chronic hepatitis C
- CXCL9, CXCL10, and CXCL11 functions are mediated by intracellular domains of CXCR3
- ductal epithelial cells produce I-TAC proteins in response to stimulation with IFNgamma secreted by lymphocytes
- novel I-TAC -599del5 promoter polymorphism is a functional variant in the presence of replicating HCV and may predispose to HCV disease susceptibility.
- recruitment might enhance the sequestration of T cells in infected lymphoid organs and the spread of infection between cells, contributing to the immunopathology of AIDS
- CXCL11 creates a chemokine gradient between the cerebrospinal fluid (CSF) and serum and recruits CXCR3-expressing memory CD4+ T-cells into the CSF of neuroborreliosis patients.
- autocrine-acting CXCL11 mediates, at least in part, the regulations of osteoclastogenesis by type I interferons
- Increased expression of the interferon-induced angiostatic ELR- CXC chemokines is a feature of juvenile DM that parallels the degree of vasculopathy in patients with the disease
- This CSCR3 ligand has the ability to activate biochemical (e.g., PtdIns and MAP kinase activation) and functional events (actin reorganization) in intestinal myofibroblasts.
- Increased I-TAC levels in blood and cerebrospinal fluid is associated with Lyme borreliosis
- CXCL11-dependent CXCR3 internalization and cell migration are regulated by the CXCR3 membrane proximal carboxyl terminus, whereas adhesion is regulated by the 3i loop S245.
- IFN-gamma promotes implantation by stimulating EEC to produce CXCL11, which induces migration of trophoblast cells and T cells, proliferation of ESC, and apoptosis of EEC.
- prolactin may enhance IFN-gamma-induced CXCL9, CXCL10, and CXCL11 production in keratinocytes
- CD13 rapidly processed CXCL11, but not CXCL8, to generate truncated CXCL11 forms that had reduced binding, signaling, and chemotactic properties for lymphocytes and CXCR3- or CXCR7-transfected cells.
- Egression of human T cells across the bronchial epithelium is a multistep process, driven in part by a polarized transepithelial gradient of CXCL11 that is up-regulated in patients with chronic obstructive airways disease.
- Data show that RIG-I mRNA and protein are expressed in HeLa cells stimulated with IFN-gamma, and that RNA interference against RIG-I results in the suppression of IFN-gamma-induced CXCL11 expression.
- DP8 cleavage of the N-terminal two residues of IP10 (CXCL10), ITAC (CXCL11) and SDF-1 (CXCL12), is reported.
- CXCL-11 is targeted by ebv-mir-BHRF1-3 in primary lymphomas
- potential new roles in down-regulating Th1 lymphocyte chemoattraction through MMP processing of CXCL11.
- There are elevated concentrations of the chemokines MDC, eotaxin, I-TAC, and MCP-1 in malignant pleural effusions.
- These data provide new structure-function dimensions for chemokines in leukocyte mobilization, disclosing an anti-inflammatory role for PAD.
- IP-9 is a key ligand in the CXCR3 signaling system for wound repair