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Validated All-in-One™ qPCR Primer for CCL4(NM_002984.3) Search again
Product ID:
HQP016625
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ACT2, AT744.1, G-26, HC21, LAG-1, LAG1, MIP-1-beta, MIP1B, MIP1B1, SCYA2, SCYA4
Gene Description:
C-C motif chemokine ligand 4
Target Gene Accession:
NM_002984.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Cholesterol is essential for macrophage inflammatory protein 1 beta binding and signaling and conformational integrity of CC chemokine receptor 5.(MIP-1 beta is the entry SCYA4 or LD78? If so, please add the term to SCYA4 or LD78.)
- results demonstrate that NH(2)-terminally truncated MIP-1 beta functions as a chemokine agonist with expanded receptor reactivity; may represent an important mechanism for regulation of immune cell recruitment during inflammatory and antiviral responses
- role in the development of osteolytic lesions in multiple myeloma
- Expression of MIP-1 beta is induced by stimulation of monocytic cells through Fc gamma receptors and involves activation of CCAAT/enhancer-binding protein beta sites.
- role of IL-12 and IFN-gamma on inducing inflammatory chemokine (MIP-1beta) secretion and down-regulating CCR5 expression in human T cells
- After stimulation via high-affinity FcepsilonRI, the transcriptional levels of I-309 (CCL1), MIP-1alpha (CCL3) and MIP-1beta (CCL4) were found among the 10 most increased human and mouse transcripts from approximately 12 000 genes
- Lymphotactin, (MIP)-1alpha, and MIP-1beta chemokines were all rapidly induced by engagement of CD28 and were calcineurin sensitive.
- MIP-1alpha and MIP-1beta show diverging signaling capacities [review]
- binding surface and affinity of monomeric and dimeric forms for various glycosaminoglycan disaccharides
- IL-1beta-induced MIP-1alpha and -1beta expression in NT2-N cells
- Cathepsin D specifically cleaves this protein that is expressed in human breast cancer.
- results suggest that endometrial macrophage inflammatory protein-1beta may be involved in the recruitment of NK cells from circulating peripheral blood
- MIP-1beta may be related to the scattering and invasion step of gastric carcinoma cells with undifferentiated phenotype
- Monocytes and B cells utilize different mechanisms to regulate expression of the 2 CCL4 genes (ACT-2 and LAG-1) & suggest that the 2 genes may not have identical activities.
- Results suggest that interleukin-1beta-mediated up-regulation of macrophage inflammatory protein-1beta production in hepatic cells may contribute to continuous recruitment of inflammatory cells to the liver and maintenance of inflammation.
- Activated T cells from GRK2+/- mice, which have a 50% reduction in GRK2 protein levels, showed a significant 40% increase in chemotaxis toward the CCR5 ligand CCL4
- MCP-1, MIP-1beta, and IL-8 elevated in relapsing polychondritis(RP) are proinflammatory chemokines, characteristic of activation of monocytes and macrophages and, in the case of IL-8, also of neutrophils. Role for cell-mediated immune response in RP.
- lymphocytes of HIV patients display a disrupted capacity to secrete the beta-chemokines MIP-1 alpha and MIP-1 beta, which may constitute a mechanism of immune dysfunction in progressive HIV infection
- produced by neonatal natural killer cells and contributes to suppession of HIV replication
- RANTES, MIP-1beta and IL-18 are expressed in LGL leukemia
- Normal human vaginal epithelial cells constitutively secrete the chemokine macrophage inflammatory protein CCL20, known to recruit Langerhans cell precursors selectively via its cognate CC chemokine receptor CCR6.
- upregulation of MIP-1beta and downregulation of VE-cadherin may strongly participate in human acute heart rejection.
- Higher amounts of MIP-1alpha, MIP-1beta, and RANTES were detected in plasma of HIV-1-positive individuals, particularly those with concomitant pulmonary tuberculosis, although the increase was not found to be statistically significant.
- variation in the CCL18-CCL3-CCL4 chemokine gene cluster influences HIV Type 1 transmission and AIDS disease progression
- Results describe the structure of an orthopoxvirus vCCI in complex with a human CC chemokine, MIP-1beta (macrophage inflammatory protein 1beta).
- findings show that CCL3, CCL4 and CCL5 bind on the Leishmania promastigotes
- the CC chemokine MIP-1beta dimer is not able to bind or activate its receptor and implicates the CC chemokine monomer as the sole receptor-interacting unit
- The strong upregulation of CCL3 and CCL4 implicates these chemokines in the etiology and pathogenesis of TLE.
- PC-PLC activation through CCR5 is specifically induced by gp120, since triggering CCR5 through its natural ligand CCL4 (MIP-1beta) does not affect PC-PLC cellular distribution and enzymatic activity, as well as CCL2 secretion
- Data show that MIP-1 alpha and MIP-1 beta bind to the HIV co-receptor CCR5 and blok HIV entry into CD4(+) lymphocytes.
- Population structure in copy number variation and SNPs in the CCL4 chemokine gene are reported.
- MCP-1, MIP-1beta, and IL-8 play important roles in the pathophysiology of Hemophagocytic lymphohistiocytosis (HLH). In addition, the serum concentrations of these chemokines may be sensitive markers for assessing disease activity in patients with HLH.
- CysLTs induce MIP-1alpha and MIP-1beta production mediated by ERK via binding to the CysLT(1) receptor in human monocytes/macrophages.
- CX3CL1, IL-15, and CCL4 can serve as independent predictors of biochemical recurrence.
- Data suggest that increased MIP-1alpha/beta production enhances multiple myeloma cell binding to stromal cells by VLA-4-VCAM-1 adhesion, forming a "vicious cycle" between MM cell adhesion to stromal cells and MIP-1 production via VLA-4-VCAM-1 interaction