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Validated All-in-One™ qPCR Primer for SCD(NM_005063.4) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an iron-containing enzyme that catalyzes a rate-limiting step in the synthesis of unsaturated fatty acids. The principal product of SCD is oleic acid, which is formed by desaturation of stearic acid. The ratio of stearic acid to oleic acid has been implicated in the regulation of cell growth and differentiation through effects on cell membrane fluidity and signal transduction. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 and SCD5 (MIM 608370), have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Zhang et al. (1999) [PubMed 10229681]; Wang et al., 2005 [PubMed 15907797]).[supplied by OMIM].
Gene References into function
- Inhibition of stearoyl-CoA desaturase activity by the cis-9,trans-11 isomer and the trans-10,cis-12 isomer of conjugated linoleic acid in human breast cancer cells.
- stearoyl-CoA desaturase influence on plasma triglycerides in hypertriglyceridemia
- Of genes identified in the liver whose expression is modulated by leptin, stearoyl-CoA desaturase-1 ranks at the top of the list, according to this review.
- relationship between triglyceride levels and the ratio of plasma oleic acid to stearic acid (the 18:1/18:0 ratio), a plasma marker of SCD activity, and n-3 PUFAs in 411 Japanese, 418 Korean, and 251 Mongolian adults
- increased palmitate and stearate desaturation by stearoyl-CoA desaturase was associated with the destabilization of ABCA1 by saturated fatty acids palmitate and stearate
- loss of SCD expression is a frequent event in prostate adenocarcinoma
- No evidence that SCD sequence variation influences diabetes susceptibility or related traits.
- Results suggest that stearoyl-CoA desaturase levels are associated with the events of neoplastic cell transformation and programmed cell death.
- by globally regulating lipid metabolism, stearoyl-CoA desaturase activity modulates cell proliferation and survival and shows the role of endogenously synthesized monounsaturated fatty acids in sustaining the neoplastic phenotype of transformed cells
- SCD activity index after rosiglitazone in PPARgamma mutation supports a pivotal role of PPARgamma function in SCD regulation.
- the lipogenic gene, stearoyl-CoA desaturase 1 (SCD1), is robustly up-regulated in skeletal muscle from extremely obese humans Elevated expression of SCD1 in skeletal muscle contributes to abnormal lipid metabolism and progression of obesity.
- SCD1 was degraded constitutively irrespective of the cellular levels of unsaturated fatty acids, which strictly regulate SCD1 gene expression.
- study demonstrated that the gene expression of COX-2 and stearoyl-coenzyme A desaturase diminished in the chronic phase of Graves'ophthalmopathy in parallel with a decrease in clinical activity score
- Genetic variations in the SCD1 gene are associated with body fat distribution and insulin sensitivity.
- The results indicate that when the supply of FA to HL60 cells is limited, the intracellular content of n-3 and n-6 FA decreases and this leads to upregulation of the desaturases, D9D is doubled.
- Results show that elevated SCD activity within adipose tissue is closely coupled to the development of insulin resistance.
- High hepatic SCD1 activity may regulate fat accumulation in the liver and possibly protects from insulin resistance in obesity.
- Fatty acid desaturation index (a marker of SCD1 activity) is a highly heritable trait that is associated with the dyslipidemia observed in familial combined hyperlipidemia.
- age-related reduction in polyunsaturated fatty acid composition was inversely correlated with SCD expression and activity resulting in elevations in monounsaturated fatty acid composition
- Changed expression of downstream PPARgamma targets after stearoyl CoA desaturase (SCD) knockdown suggests that PPARgamma up-regulation of SCD leads to increased lipogenesis and potentiation of adiponectin signaling.
- SCD1 activity regulates Akt activation in human lung adenocarcinoma cells
- Dyslipidemia and atherosclerosis induced by chronic intermittent hypoxia are attenuated by deficiency of stearoyl coenzyme A desaturase.
- associations of Delta 9 with adiposity and plasma lipids in healthy female adolescents support the concept derived from rodent models that Delta 9 activity is independently reflective of higher body mass index and higher circulatory triglyceride levels